Anti-TBR2 / Eomes 抗体 [EPR19012] (ab183991)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.co.jp/ps/products/183/ab183991/Images/ab183991-266946-anti-tbr2-eomes-antibody-epr19012-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19012] to TBR2 / Eomes
- Suitable for: ICC/IF, IHC-Fr, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-TBR2 / Eomes antibody [EPR19012]
TBR2 / Eomes 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR19012] to TBR2 / Eomes -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Fr, IHC-Pmore details -
種交差性
交差種: Mouse
非交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-P: Mouse hippocampus and E14 cortex tissues; IHC-Fr: Mouse E14.5 cortex and hippocampus tissues; ICC/IF: Mouse primary neuron cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19012 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab183991の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/200.
|
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| IHC-Fr | (4) |
1/1000.
Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). |
| IHC-P | (1) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
ICC/IF
1/200. |
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IHC-Fr
1/1000. Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. -
組織特異性
Expressed in CD8+ T-cells. -
関連疾患
Note=A translocation t(3;10)(p24;q23) located 215 kb 3' to the EOMES gene but leading to loss of its expression was identified in a large consanguineous family. Homozygous silencing produces microcephaly associated with corpus callosum agenesis, bilateral polymicrogyria, ventricular dilatation and a small cerebellum. -
配列類似性
Contains 1 T-box DNA-binding domain. -
発生段階
Detected at 7 weeks of development in the forebrain floorplate of the CNS. Expressed within the mantle layer and migrating neuroblasts of the telencephalon at 12.5 weeks of development. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 13813 Mouse
- SwissProt: O54839 Mouse
- Unigene: 200692 Mouse
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別名
- eomes antibody
- EOMES_HUMAN antibody
- Eomesodermin antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling TBR2 / Eomes with ab183991 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on intermediate neuronal progenitors of mouse hippocampus is observed [PMID: 22553033].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.co.jp/ps/products/183/ab183991/Images/ab183991-7-anti-tbr2eomes-antibody-epr19012-immunocytochemistry-mouse-primary-neuron-cell1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling TBR2 / Eomes with ab183991 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nuclear staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).
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![Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.co.jp/ps/products/183/ab183991/Images/ab183991-266944-anti-tbr2-eomes-antibody-epr19012-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 cortex tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Specific staining on E14.5 mouse cortex is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.co.jp/ps/products/183/ab183991/Images/ab183991-266945-anti-tbr2-eomes-antibody-epr19012-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)Immunohistochemical analysis of paraffin-embedded mouse E14 cortex tissue labeling TBR2 / Eomes with ab183991 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on the cortex of mouse E14 is observed [PMID: 22553033].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.co.jp/ps/products/183/ab183991/Images/ab183991-266943-anti-tbr2-eomes-antibody-epr19012-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Specific staining on adult mouse hippocampus is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
-
![Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.co.jp/ps/products/183/ab183991/Images/ab183991-266942-anti-tbr2-eomes-antibody-epr19012-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen adult mouse kidney tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Negative control tissue; very weak staining is due to autoflorescence of the kidney section.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.co.jp/ps/products/183/ab183991/Images/ab183991-8-benefits-of-recombinant-antibodies.png)
Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (50)
ab183991 は 50 報の論文で使用されています。
- Lee D et al. Molecular and Immune Profiling of Syngeneic Mouse Models Predict Response to Immune Checkpoint Inhibitors in Gastric Cancer. Cancer Res Treat 55:167-178 (2023). PubMed: 35609622
- Tominaga K et al. Tip60/KAT5 Histone Acetyltransferase Is Required for Maintenance and Neurogenesis of Embryonic Neural Stem Cells. Int J Mol Sci 24:N/A (2023). PubMed: 36768434
- Fujimura K et al. Integrative systems biology characterizes immune-mediated neurodevelopmental changes in murine Zika virus microcephaly. iScience 26:106909 (2023). PubMed: 37332674
- Yuan L et al. Camrelizumab combined with apatinib in patients with first-line platinum-resistant or PD-1 inhibitor resistant recurrent/metastatic nasopharyngeal carcinoma: a single-arm, phase 2 trial. Nat Commun 14:4893 (2023). PubMed: 37580352
- Leiter O et al. Platelet-derived exerkine CXCL4/platelet factor 4 rejuvenates hippocampal neurogenesis and restores cognitive function in aged mice. Nat Commun 14:4375 (2023). PubMed: 37587147