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リコンビナントRabMAb

Anti-Tau (phospho T217) 抗体 [EPR24654-110] (BSA and Azide free) (ab291104)

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Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)
  • Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)
  • Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)
  • Immunoprecipitation - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)
  • Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)
  • Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)
  • Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24654-110] to Tau (phospho T217) - BSA and Azide free
  • Suitable for: WB, IP, Indirect ELISA, ICC/IF, Dot blot
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free)
    Tau 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24654-110] to Tau (phospho T217) - BSA and Azide free

  • 由来種

    Rabbit

  • 特異性

    The specificity of this antibody refers to P10636-8.

  • アプリケーション

    適用あり: WB, IP, Indirect ELISA, ICC/IF, Dot blotmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Mouse, Human
    非交差種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse P7 hippocampus tissue lysate, and mouse P5 brain tissue lysate (Phosphatase treated and untreated membrane). ICC/IF: mouse primary neurons. IP: Mouse P14 brain tissue lysate. ELISA: Human Tau (phospho T217) peptide. Dot blot: Tau (phospho T217) peptide.

  • 特記事項

    ab291104 is the carrier-free version of ab291080.

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab291104の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 50-80 kDa (predicted molecular weight: 46 kDa).
IP
Use at an assay dependent concentration.
Indirect ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 50-80 kDa (predicted molecular weight: 46 kDa).
IP
Use at an assay dependent concentration.
Indirect ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

  • 組織特異性

    Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.

  • 関連疾患

    Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
    Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
    Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
    Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
    Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613.

  • 配列類似性

    Contains 4 Tau/MAP repeats.

  • 発生段階

    Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.

  • ドメイン

    The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.

  • 翻訳後修飾

    Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
    Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
    Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.

  • 細胞内局在

    Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
  • Information by UniProt

  • 参照データベース

    • 製品の状態

      There are 9 isoforms produced by alternative splicing.

    • 別名

      • AI413597 antibody
      • AW045860 antibody
      • DDPAC antibody
      • FLJ31424 antibody
      • FTDP 17 antibody
      • G protein beta1/gamma2 subunit interacting factor 1 antibody
      • MAPT antibody
      • MAPTL antibody
      • MGC134287 antibody
      • MGC138549 antibody
      • MGC156663 antibody
      • Microtubule associated protein tau antibody
      • Microtubule associated protein tau isoform 4 antibody
      • Microtubule-associated protein tau antibody
      • MSTD antibody
      • Mtapt antibody
      • MTBT1 antibody
      • MTBT2 antibody
      • Neurofibrillary tangle protein antibody
      • Paired helical filament tau antibody
      • Paired helical filament-tau antibody
      • PHF tau antibody
      • PHF-tau antibody
      • PPND antibody
      • PPP1R103 antibody
      • Protein phosphatase 1, regulatory subunit 103 antibody
      • pTau antibody
      • RNPTAU antibody
      • TAU antibody
      • TAU_HUMAN antibody
      • Tauopathy and respiratory failure antibody
      • Tauopathy and respiratory failure, included antibody
      see all

    画像

    • Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)
      Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)
      All lanes : Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080) at 1/1000 dilution

      Lane 1 : Mouse P7 hippocampus tissue lysate (Untreated membrane)
      Lane 2 : Mouse P7 hippocampus tissue lysate (Phosphatase treated membrane)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 46 kDa
      Observed band size: 50-80 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      This data was developed using ab291080, the same antibody clone in a different buffer formulation.

      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    • Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)
      Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)
      All lanes : Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080) at 1/1000 dilution

      Lane 1 : Mouse P5 brain tissue lysate (Untreated membrane)
      Lane 2 : Mouse P5 brain tissue lysate (Phosphatase treated membrane)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

      Developed using the ECL technique.

      Predicted band size: 46 kDa
      Observed band size: 50-80 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      This data was developed using ab291080, the same antibody clone in a different buffer formulation.

      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    • Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)
      Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)

      This data was developed using ab291080, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized mouse primary neurons labelling Tau (phospho T217) with ab291080 at 1/2000 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (green). Confocal image showing the positive staining in mouse primary neuron, and the signal decreased after phosphatase treatment at 37℃ for 2 h. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Counterstain primary antibody used was ab11267 Anti-MAP2 mouse monoclonal antibody together with secondary, ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Red). The nuclear counter stain is DAPI (blue).

    • Immunoprecipitation - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)
      Immunoprecipitation - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)

      This data was developed using ab291080, the same antibody clone in a different buffer formulation.

      Tau (phospho T217) was immunoprecipitated from 0.35 mg mouse P14 brain tissue lysate with ab291080 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab291080 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse P14 brain tissue lysate 10 µg (Input)

      Lane 2: ab291080 IP in Mouse P14 brain tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab291080 in mouse P14 brain tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 32 seconds

    • Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)
      Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)

      This data was developed using ab291080, the same antibody clone in a different buffer formulation.

      Antigen human Tau (phospho T217) peptide and  a control human Tau non-phospho peptide were at 1000 ng/ml.  ab291080 was used at 1000-0 ng/ml.  Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1/2500 dilution.

    • Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)
      Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)

      This data was developed using ab291080, the same antibody clone in a different buffer formulation.

      Concentration of ab291080: 1/1000 dilution (0.58 μg/ml)

      Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/100,000 dilution

      Blocking/diluting buffer and concentration: 5% NFDM/TBST

      Lane 1: Tau (phospho T217) peptide

      Lane 2: Tau (phospho T220) peptide

      Lane 3: Tau non-phospho T217 peptide

      Exposure time: 3 minutes

    • Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)
      Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) (ab291104)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab291104 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab291104 は論文での使用が確認できていません。

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