JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB4841

Anti-Tau (phospho T205) 抗体

Anti-Tau (phospho T205) antibody

Be the first to review this product! Submit a review

|

(15 Publications)

Rabbit Polyclonal TAU phospho T205 antibody. Suitable for Flow Cyt, WB, IHC-Fr and reacts with Human, Recombinant full length protein, Mouse samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human MAPT phospho T205.

別名を表示する

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

2 Images
Flow Cytometry - Anti-Tau (phospho T205) antibody (AB4841)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Tau (phospho T205) antibody (AB4841)

Flow cytometric analysis of 70% ethanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling Tau (phospho T205) with ab4841 at 5 μg/million cells (red) compared with a rabbit Isotype control details (pink),an unlabelled control (purple) and a secondary only control (green). Alexa Fluor® 488 Goat Anti-Rabbit, at 1/400 dilution was used as the secondary antibody.

Western blot - Anti-Tau (phospho T205) antibody (AB4841)
  • WB

Unknown

Western blot - Anti-Tau (phospho T205) antibody (AB4841)

WB using ab4841. The peptide corresponding to tau [pT205] blocks the antibody signal, thereby demonstrating the specificity of the antibody.

All lanes:

Western blot - Anti-Tau (phospho T205) antibody (ab4841)

Predicted band size: 78 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Mouse

アプリケーション

IHC-Fr, WB, Flow Cyt

applications

免疫原

Synthetic Peptide within Human MAPT phospho T205. The exact immunogen used to generate this antibody is proprietary information.

P10636

特異性

The specificity of this antibody refers to P10636-8.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µg for 10^5 Cells", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Recombinant full length protein": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated tau. The final product is generated by affinity chromatography using a tau-derived peptide that is phosphorylated at threonine 205.
バッファー組成
pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
See full target information MAPT phospho T205
style
left-column

文献 (15)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0152224 PubMed39189744

2024

HSV-1 infection induces phosphorylated tau propagation among neurons via extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

V Protto,M T Miteva,F Iannuzzi,M E Marcocci,D D Li Puma,R Piacentini,M Belli,L Sansone,A Pietrantoni,C Grassi,A T Palamara,G De Chiara

Science advances 8:eabl8809 PubMed35857446

2022

Alzheimer's disease: Ablating single master site abolishes tau hyperphosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Kristie Stefanoska,Mehul Gajwani,Amanda R P Tan,Holly I Ahel,Prita R Asih,Alexander Volkerling,Anne Poljak,Arne Ittner

International journal of molecular sciences 23: PubMed35216271

2022

Detection of Pathological Markers of Neurodegenerative Diseases following Microfluidic Direct Conversion of Patient Fibroblasts into Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Cristiana Mollinari,Chiara De Dominicis,Leonardo Lupacchini,Luigi Sansone,Davide Caprini,Carlo Massimo Casciola,Ying Wang,Jian Zhao,Massimo Fini,Matteo Russo,Enrico Garaci,Daniela Merlo

iScience 24:102993 PubMed34505007

2021

Vascular smooth muscle cell dysfunction contribute to neuroinflammation and Tau hyperphosphorylation in Alzheimer disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge A Aguilar-Pineda,Karin J Vera-Lopez,Pallavi Shrivastava,Miguel A Chávez-Fumagalli,Rita Nieto-Montesinos,Karla L Alvarez-Fernandez,Luis D Goyzueta Mamani,Gonzalo Davila Del-Carpio,Badhin Gomez-Valdez,Clint L Miller,Rajeev Malhotra,Mark E Lindsay,Christian L Lino Cardenas

Progress in neurobiology 197:101900 PubMed32841723

2020

Amelioration of Tau pathology and memory deficits by targeting 5-HT7 receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Josephine Labus,Kian-Fritz Röhrs,Jana Ackmann,Hristo Varbanov,Franziska E Müller,Shaobo Jia,Kathrin Jahreis,Anna-Lena Vollbrecht,Malte Butzlaff,Yvonne Schill,Daria Guseva,Katrin Böhm,Rahul Kaushik,Monika Bijata,Philippe Marin,Séverine Chaumont-Dubel,Andre Zeug,Alexander Dityatev,Evgeni Ponimaskin

Theranostics 10:8430-8445 PubMed32724479

2020

Modulation of the Astrocyte-Neuron Lactate Shuttle System contributes to Neuroprotective action of Fibroblast Growth Factor 21.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Sun,Yue Wang,Su-Ting Chen,Ying-Jie Chen,Jie Shen,Wen-Bing Yao,Xiang-Dong Gao,Song Chen

Frontiers in cellular neuroscience 13:239 PubMed31263400

2019

Activated PPARγ Abrogates Misprocessing of Amyloid Precursor Protein, Tau Missorting and Synaptotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Moosecker,Patrícia Gomes,Chrysoula Dioli,Shuang Yu,Ioannis Sotiropoulos,Osborne F X Almeida

Neurobiology of aging 76:80-95 PubMed30708185

2019

Progressive signaling changes in the olfactory nerve of patients with Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Praveen Bathini,Antoine Mottas,Muriel Jaquet,Emanuele Brai,Lavinia Alberi

International journal of molecular medicine 43:435-442 PubMed30365112

2018

Amyloid β and tau are involved in sleep disorder in Alzheimer's disease by orexin A and adenosine A(1) receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Liu,Fumin Wang,Minglu Tang,Yongjun Zhao,Xiaoting Wang

Acta neuropathologica communications 4:64 PubMed27364742

2016

Notch1 hallmarks fibrillary depositions in sporadic Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Emanuele Brai,Noemi Alina Raio,Lavinia Alberi
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB254410

Anti-Tau (phospho T205) antibody [EPR23505-13]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Indirect ELISA - Anti-Tau (phospho T205) antibody [EPR23505-13] (AB254410)

  • 0
  • 0
Primary Antibodies

AB92682

Anti-Tau (phospho S356) antibody [EPR2603]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Tau (phospho S356) antibody [EPR2603] (AB92682)

  • 0
  • 0
Primary Antibodies

AB109390

Anti-Tau (phospho S396) antibody [EPR2731]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (AB109390)

  • 4
  • 13
Primary Antibodies

AB151559

Anti-Tau (phospho T231) antibody [EPR2488]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T231) antibody [EPR2488] (AB151559)

  • 5
  • 6
Primary Antibodies

AB32057

Anti-Tau (phospho S396) antibody [E178]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (AB32057)

  • 5
  • 5
Primary Antibodies

AB170892

Anti-Tau (phospho S214) antibody [EPR1884(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

  • 5
  • 1
Primary Antibodies

AB92676

Anti-Tau (phospho S404) antibody [EPR2605]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (AB92676)

  • 5
  • 4
Primary Antibodies

AB93726

Anti-Tau antibody [SP70]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [SP70] (AB93726)

  • 5
  • 4

Complementary Products

Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
  • 19
Primary Antibodies

AB32136

Anti-Amyloid Precursor Protein antibody [Y188]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Precursor Protein antibody [Y188] (AB32136)

  • 4
  • 9
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB210703

Anti-Tau (phospho S202 + T205) antibody [EPR20390]

20ul selling size|RabMAb|Recombinant|Trial Size

Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (AB210703)

  • 3
  • 3

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com