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AB223192

Anti-Tau (phospho T181) 抗体 [EPR22064]

Anti-Tau (phospho T181) antibody [EPR22064]

5

(4 Reviews)

|

(1 Publication)

Rabbit Recombinant Monoclonal TAU phospho T181 antibody. Suitable for IP, Dot, WB, I-ELISA and reacts with Human, Synthetic peptide, Synthetic peptide - Human samples. Cited in 1 publication.

別名を表示する

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

4 Images
Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)
  • IP

Unknown

Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)

Tau (phospho T181 was immunoprecipitated from 0.35 mg of human brain lysate with ab223192 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223192 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human brain lysate 10 μg (Input).
Lane 2 : ab223192 IP in Human brain lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab223192 in Human brain lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST
Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR22064] (ab223192) at 1/500 dilution

Predicted band size: 79 kDa

Observed band size: 60 kDa

false

Indirect ELISA - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)
  • I-ELISA

Lab

Indirect ELISA - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)

Indirect ELISA analysis of ab223192 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Human MAPT(phospho T181) peptide, Human MAPT non-phospho peptide

Antigen concentration : 1000 ng/ml.

Western blot - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)
  • WB

Unknown

Western blot - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)

Blocking and dilution buffer : 5% NFDM/TBST.

WB membrane in Lane 2 was treated with alkaline phosphatase for 1h at 37°C.

All lanes:

Western blot - Anti-Tau (phospho T181) antibody [EPR22064] (ab223192) at 1/1000 dilution

Lane 1:

Human brain lysate at 10 µg

Lane 2:

Human brain lysate (WB membrane treated with alkaline phosphatase) at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 79 kDa

Observed band size: 60 kDa

false

Exposure time: 3min

Dot Blot - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)
  • Dot

Unknown

Dot Blot - Anti-Tau (phospho T181) antibody [EPR22064] (AB223192)

Dot blot analysis of Tau (phospho T181) labeled with ab223192 at 1/1000 dilution.

Lane 1 : Tau (phospho T181) peptide.
Lane 2 : Tau non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR22064

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

Dot, IP, I-ELISA, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

The specificity of this antibody refers to P10636-8.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Synthetic peptide - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "62.5 ng/mL", "IELISA-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT phospho T181
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 644:1058-1068 PubMed40659786

2025

Microbiota-driven antitumour immunity mediated by dendritic cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Yi-Tzu Lin,Shota Fukuoka,Shohei Koyama,Daisuke Motooka,Dieter M Tourlousse,Yuko Shigeno,Yuki Matsumoto,Hiroyuki Yamano,Kazutoshi Murotomi,Hideyuki Tamaki,Takuma Irie,Eri Sugiyama,Shogo Kumagai,Kota Itahashi,Tokiyoshi Tanegashima,Kaori Fujimaki,Sachiko Ito,Mariko Shindo,Takahiro Tsuji,Hiroaki Wake,Keisuke Watanabe,Yuka Maeda,Tomohiro Enokida,Makoto Tahara,Riu Yamashita,Takao Fujisawa,Motoo Nomura,Akihito Kawazoe,Koichi Goto,Toshihiko Doi,Kohei Shitara,Hiroyuki Mano,Yuji Sekiguchi,Shota Nakamura,Yoshimi Benno,Hiroyoshi Nishikawa
View all publications
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