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AB109401

Anti-Tau (phospho S324) 抗体 [EPR2457(2)]

Anti-Tau (phospho S324) antibody [EPR2457(2)]

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(7 Publications)

Rabbit Recombinant Monoclonal TAU phospho S324 antibody. Suitable for WB, ICC/IF, I-ELISA and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

別名を表示する

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, ptau, p tau, phospho tau, MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S324) antibody [EPR2457(2)] (AB109401)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S324) antibody [EPR2457(2)] (AB109401)

Immunofluorescent staining of Tau in SH-SY5Y cells using ab109401 at 1/100.

Indirect ELISA - Anti-Tau (phospho S324) antibody [EPR2457(2)] (AB109401)
  • I-ELISA

Lab

Indirect ELISA - Anti-Tau (phospho S324) antibody [EPR2457(2)] (AB109401)

Indirect ELISA analysis of ab109401 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human Tau (phospho S324) peptide, Human Tau non-phospho peptide.

Antigen concentration : 100 ng/ml

Western blot - Anti-Tau (phospho S324) antibody [EPR2457(2)] (AB109401)
  • WB

Lab

Western blot - Anti-Tau (phospho S324) antibody [EPR2457(2)] (AB109401)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Tau antibody [EP2456Y] (ab76128) Total Protein Control at 1/1000 dilution.

All lanes:

Western blot - Anti-Tau (phospho S324) antibody [EPR2457(2)] (ab109401) at 1/1000 dilution

Lane 1:

Untreated mouse brain tissue lysate (untreated membrane) at 20 µg

Lane 2:

Mouse brain treated with 1μM Okadaic Acid and 200nM Calyculin A for 1 hour, tissue lysate (untreated membrane) at 20 µg

Lane 3:

Untreated mouse brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 4:

Mouse brain treated with 1μM Okadaic Acid and 200nM Calyculin A for 1 hour, tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 48-60 kDa

false

Exposure time: 136s

Western blot - Anti-Tau (phospho S324) antibody [EPR2457(2)] (AB109401)
  • WB

Lab

Western blot - Anti-Tau (phospho S324) antibody [EPR2457(2)] (AB109401)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Tau antibody [EP2456Y] (ab76128) Total Protein Control at 1/1000 dilution.

All lanes:

Western blot - Anti-Tau (phospho S324) antibody [EPR2457(2)] (ab109401) at 1/1000 dilution

Lane 1:

Untreated rat brain tissue lysate (untreated membrane) at 20 µg

Lane 2:

Rat brain treated with 1μM Okadaic Acid and 200nM Calyculin A for 1 hour, tissue lysate (untreated membrane) at 20 µg

Lane 3:

Untreated rat brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 4:

Rat brain treated with 1μM Okadaic Acid and 200nM Calyculin A for 1 hour, tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 48-60 kDa

false

Exposure time: 70s

Western blot - Anti-Tau (phospho S324) antibody [EPR2457(2)] (AB109401)
  • WB

Unknown

Western blot - Anti-Tau (phospho S324) antibody [EPR2457(2)] (AB109401)

All lanes:

Western blot - Anti-Tau (phospho S324) antibody [EPR2457(2)] (ab109401) at 1/10000 dilution

Lane 1:

SH-SY5Y cell lysate untreated at 10 µg

Lane 2:

SH-SY5Y cell lysate treated with Okadaic acid and Calyculin A at 10 µg

Predicted band size: 78 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Tau (phospho S324) antibody [EPR2457(2)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR2457(2)

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Mouse, Rat

アプリケーション

WB, ICC/IF, I-ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

The specificity of this antibody refers to P10636-8&#46;

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代替製品

Primary Antibodies

AB92682

Anti-Tau (phospho Ser356) antibody [EPR2603]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Tau (phospho Ser356) antibody [EPR2603] (AB92682)

  • 0
  • 0
Primary Antibodies

AB109390

Anti-Tau (phospho S396) antibody [EPR2731]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (AB109390)

  • 4
  • 14
Primary Antibodies

AB151559

Anti-Tau (phospho T231) antibody [EPR2488]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (AB151559)

  • 5
  • 6
Primary Antibodies

AB32057

Anti-Tau (phospho S396) antibody [E178]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (AB32057)

  • 5
  • 5
Primary Antibodies

AB170892

Anti-Tau (phospho S214) antibody [EPR1884(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

  • 5
  • 1
Primary Antibodies

AB92676

Anti-Tau (phospho S404) antibody [EPR2605]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (AB92676)

  • 5
  • 4
Primary Antibodies

AB93726

Anti-Tau antibody [SP70]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [SP70] (AB93726)

  • 5
  • 4

組合せ製品

Primary Antibodies

AB75603

Anti-Tau (Phospho Ser673) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Tau (Phospho Ser673) Antibody (AB75603)

  • 0
  • 0
Primary Antibodies

AB76128

Anti-Tau antibody [EP2456Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Western blot - Anti-Tau antibody [EP2456Y] (AB76128)

  • 5
  • 1
Primary Antibodies

AB170892

Anti-Tau (phospho S214) antibody [EPR1884(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

  • 5
  • 1
Primary Antibodies

AB131354

Anti-Tau (phospho S262) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S262) antibody (AB131354)

  • 3
  • 2
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Reactivity data

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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT pS324
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1139 PubMed40745206

2025

Stoichiometric 14-3-3ζ binding promotes phospho-Tau microtubule dissociation and reduces aggregation and condensation.

Applications

Unspecified application

Species

Unspecified reactive species

Janine Hochmair,Maxime C M van den Oetelaar,Leandre Ravatt,Lisa Diez,Lenne J M Lemmens,Renata Ponce-Lina,Rithika Sankar,Maximilian Franck,Gesa Nolte,Ekaterina Semenova,Satabdee Mohapatra,Christian Ottmann,Luc Brunsveld,Susanne Wegmann

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2310134 PubMed38634567

2024

Tau Aggregation-Dependent Lipid Peroxide Accumulation Driven by the hsa_circ_0001546/14-3-3/CAMK2D/Tau Complex Inhibits Epithelial Ovarian Cancer Peritoneal Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

BinShu Chai,Yong Wu,HengHui Yang,BiaoFeng Fan,SiYu Cao,XiaoFei Zhang,YaQing Xie,ZhiXiang Hu,ZhongLiang Ma,YunKui Zhang,Wei Pan,Wei Meng,Jiao Meng,WenJuan Tian,JiaLi Zhang,YanLi Li,Yang Shao,ShaoJia Wang

Journal of neuroscience research 101:1900-1913 PubMed37787045

2023

Breast-to-brain metastasis is exacerbated with chemotherapy through blood-cerebrospinal fluid barrier and induces Alzheimer's-like pathology.

Applications

Unspecified application

Species

Unspecified reactive species

B Saatian,K Deshpande,R Herrera,S Sedighi,R Eisenbarth,M Iyer,D Das,A Julian,V Martirosian,A Lowman,P LaViolette,J Remsik,A Boire,E Sankey,P E Fecci,M S Shiroishi,F Chow,K Hurth,J Neman

Molecular metabolism 73:101731 PubMed37121509

2023

Semaphorin 4B is an ADAM17-cleaved adipokine that inhibits adipocyte differentiation and thermogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulbasit Amin,Marina Badenes,Johanna Tüshaus,Érika de Carvalho,Emma Burbridge,Pedro Faísca,Květa Trávníčková,André Barros,Stefania Carobbio,Pedro M Domingos,Antonio Vidal-Puig,Luís F Moita,Sarah Maguire,Kvido Stříšovský,Francisco J Ortega,José Manuel Fernández-Real,Stefan F Lichtenthaler,Colin Adrain

The EMBO journal 41:e108882 PubMed35298090

2022

Molecular crowding and RNA synergize to promote phase separation, microtubule interaction, and seeding of Tau condensates.

Applications

Unspecified application

Species

Unspecified reactive species

Janine Hochmair,Christian Exner,Maximilian Franck,Alvaro Dominguez-Baquero,Lisa Diez,Hévila Brognaro,Matthew L Kraushar,Thorsten Mielke,Helena Radbruch,Senthilvelrajan Kaniyappan,Sven Falke,Eckhard Mandelkow,Christian Betzel,Susanne Wegmann

The Journal of biological chemistry 296:100760 PubMed33965374

2021

TBK1 interacts with tau and enhances neurodegeneration in tauopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Measho H Abreha,Shamsideen Ojelade,Eric B Dammer,Zachary T McEachin,Duc M Duong,Marla Gearing,Gary J Bassell,James J Lah,Allan I Levey,Joshua M Shulman,Nicholas T Seyfried

PloS one 14:e0225727 PubMed31794565

2019

The KLDpT activation loop motif is critical for MARK kinase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Tim Sonntag,James J Moresco,John R Yates,Marc Montminy
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
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