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リコンビナントRabMAb

Anti-Tau (phospho S198) 抗体 [EPR2400] (ab79540)

Submit a review Submit a question References (5)
Western blot - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)
  • Immunoprecipitation - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)
  • Western blot - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)
  • Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2400] to Tau (phospho S198)
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Tau (phospho S198) antibody [EPR2400]
    Tau 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2400] to Tau (phospho S198)

  • 由来種

    Rabbit

  • 特異性

    The specificity of this antibody refers to P10636-8.

  • アプリケーション

    適用あり: WB, IP, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse hippocampus and Rat hippocampus tissues. SH-SY5Y (cells treated with 1µM okadaic acid and 200nM calyculin a for 60 minutes) whole cell lysate. IP: SH-SY5Y cell lysate. IHC-P: Mouse cerebrum, Rat cerebrum, and Human breast cancer tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab79540の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 50-70 kDa (predicted molecular weight: 79 kDa).

For unpurified use at 1/50000 - 1/100000.
IP
1/50.
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.
特記事項
WB
1/1000. Detects a band of approximately 50-70 kDa (predicted molecular weight: 79 kDa).

For unpurified use at 1/50000 - 1/100000.
IP
1/50.
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

ターゲット情報

  • 機能

    Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

  • 組織特異性

    Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.

  • 関連疾患

    Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
    Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
    Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
    Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
    Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613.

  • 配列類似性

    Contains 4 Tau/MAP repeats.

  • 発生段階

    Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.

  • ドメイン

    The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.

  • 翻訳後修飾

    Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
    Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
    Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.

  • 細胞内局在

    Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
  • Information by UniProt

  • 参照データベース

    see all

  • 製品の状態

    There are 9 isoforms produced by alternative splicing.

  • 別名

    • AI413597 antibody
    • AW045860 antibody
    • DDPAC antibody
    • FLJ31424 antibody
    • FTDP 17 antibody
    • G protein beta1/gamma2 subunit interacting factor 1 antibody
    • MAPT antibody
    • MAPTL antibody
    • MGC134287 antibody
    • MGC138549 antibody
    • MGC156663 antibody
    • Microtubule associated protein tau antibody
    • Microtubule associated protein tau isoform 4 antibody
    • Microtubule-associated protein tau antibody
    • MSTD antibody
    • Mtapt antibody
    • MTBT1 antibody
    • MTBT2 antibody
    • Neurofibrillary tangle protein antibody
    • Paired helical filament tau antibody
    • Paired helical filament-tau antibody
    • PHF tau antibody
    • PHF-tau antibody
    • PPND antibody
    • PPP1R103 antibody
    • Protein phosphatase 1, regulatory subunit 103 antibody
    • pTau antibody
    • RNPTAU antibody
    • TAU antibody
    • TAU_HUMAN antibody
    • Tauopathy and respiratory failure antibody
    • Tauopathy and respiratory failure, included antibody
    see all

画像

  • Western blot - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)
    Western blot - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)
    All lanes : Anti-Tau (phospho S198) antibody [EPR2400] (ab79540) at 1/10000 dilution (Purified)

    Lane 1 : Mouse hippocampus lysate
    Lane 2 : Mouse hippocampus lysate, the membrane treated with Alkaline Phosphatase for 1 hour
    Lane 3 : Rat hippocampus lysate
    Lane 4 : Rat hippocampus lysate, the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa
    Observed band size: 50-70 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Tau with purified ab79540 at 1:1000 (0.423 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. Postive staining on rat cerebrum without alkaline phosphatase treatment (image A). No staining on rat cerebrum with alkaline phosphatase treatment (image B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunoprecipitation - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)
    Immunoprecipitation - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)

    Purified ab79540 at 1/50 dilution (2µg) immunoprecipitating Tau in SH-SY5Y whole cell lysate.
    Lane 1 (input): SY-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab79540 + SH-SY5Y whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab79540 in SH-SY5Y whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 50-70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Tau with purified ab79540 at 1:1000 (0.423 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. Postive staining on mouse cerebrum without alkaline phosphatase treatment (image A). No staining on mouse cerebrum with alkaline phosphatase treatment (image B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)
    Western blot - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)
    All lanes : Anti-Tau (phospho S198) antibody [EPR2400] (ab79540) at 1/1000 dilution (Purified)

    Lane 1 : Untreated SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
    Lane 2 : SH-SY5Y treated with 1µM okadaic acid and 200nM calyculin a for 60 minutes, whole cell lysate
    Lane 3 : SH-SY5Y treated with 1µM okadaic acid and 200nM calyculin a for 60 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa
    Observed band size: 50-70 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling Tau with purified ab79540 at 1:1000 (0.423 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. Postive staining on human breast cancer without alkaline phosphatase treatment (image A). No staining on human breast cancer with alkaline phosphatase treatment (image B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)
    Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)

参考文献 (5)

ab79540 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab79540 は 5 報の論文で使用されています。

  • Chen W  et al. Astrocytic Insulin-Like Growth Factor-1 Protects Neurons Against Excitotoxicity. Front Cell Neurosci 13:298 (2019). PubMed: 31338023
  • Ercan-Herbst E  et al. A post-translational modification signature defines changes in soluble tau correlating with oligomerization in early stage Alzheimer's disease brain. Acta Neuropathol Commun 7:192 (2019). PubMed: 31796124
  • Ahmad W Dihydrolipoamide dehydrogenase suppression induces human tau phosphorylation by increasing whole body glucose levels in a C. elegans model of Alzheimer's Disease. Exp Brain Res N/A:N/A (2018). PubMed: 30056470
  • Fernius J  et al. Human TTBK1, TTBK2 and MARK1 kinase toxicity in Drosophila melanogaster is exacerbated by co-expression of human Tau. Biol Open 6:1013-1023 (2017). WB . PubMed: 28711868
  • Ercan E  et al. A validated antibody panel for the characterization of tau post-translational modifications. Mol Neurodegener 12:87 (2017). WB, ICC/IF, PepArr . PubMed: 29157277

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