Anti-TATA binding protein TBP 抗体 [mAbcam51841] (BSA and Azide free) (ab282715)

This data was developed using ab300656, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 

Lanes 1-6: 15 seconds;
Lanes 7-8: 5.5 seconds;
Lane 9: 3 minutes.

This data was developed using ab300656, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon labeling TATA binding protein TBP with ab300656 at 1/10000 dilution (0.103 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on human colon. The section was incubated with ab300656 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

This data was developed using ab300656, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma labeling TATA binding protein TBP with ab300656 at 1/10000 dilution (0.103 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on human colon carcinoma. The section was incubated with ab300656 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

This data was developed using ab300656, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse stomach labeling TATA binding protein TBP with ab300656 at 1/10000 dilution (0.103 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on mouse stomach. The section was incubated with ab300656 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

This data was developed using ab300656, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat colon labeling TATA binding protein TBP with ab300656 at 1/10000 dilution (0.103 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on rat colon. The section was incubated with ab300656 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

This data was developed using ab300656, the same antibody clone in a different buffer formulation.

TATA binding protein TBP was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate (10 µg) with ab300656 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300656 at 1/2000 dilution. Mouse IgG for IP (HRP) (ab131368) was used as the secondary at 1/5000 dilution.

Lane 1(Input): HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg

Lane 2(+): HeLa whole cell lysate

Lane 3(-): Mouse monoclonal IgG2c (ab170191) instead of ab51841 in HeLa whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 24 seconds

This data was developed using ab300656, the same antibody clone in a different buffer formulation.

TATA binding protein TBP was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (10 µg) with ab300656 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300656 at 1/2000 dilution. Mouse IgG for IP (HRP) (ab131368) was used as the secondary at 1/5000 dilution.

Lane 1(Input): NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 μg

Lane 2(+): NIH/3T3 whole cell lysate

Lane 3(-): Mouse monoclonal IgG2c (ab170191) instead of ab51841 in NIH/3T3 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 24 seconds

This data was developed using ab300656, the same antibody clone in a different buffer formulation.

Chromatin was prepared from HeLa (Human cervix adenocarcinoma epithelial cells) according to the Abcam Dual-X-ChIP protocol. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min. The ChIP was performed with 25 µg of chromatin, 5 µg of ab300656 (red), or 5 µg of mouse IgG2c ab170191 (gray) and 25 µl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach).

Cross-linking conditions: 1.5mM EGS for 30mins, then 1% formaldehyde for 10mins

*http://www.abcam.com/resources?keywords=X%20ChIP%20protocol