AB282715

Anti-TATA binding protein TBP 抗体 [mAbcam51841] (BSA and Azide free)

Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free)

BOND RX™ Validated
Recombinant
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Mouse Recombinant Monoclonal TATA binding protein TBP antibody. Carrier free. Suitable for ChIP, IP, IHC-P, WB and reacts with Human, Mouse, Rat samples.

別名を表示する

GTF2D1, TF2D, TFIID, TBP, TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit

7 Images

Supplier Data

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

This data was developed using ab300656, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon carcinom tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on human colon carcinoma. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Supplier Data

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

This data was developed using ab300656, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on human colon. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond® Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Lab

Immunoprecipitation - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

This data was developed using ab300656, the same antibody clone in a different buffer formulation. TATA binding protein TBP was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate (10 µg) with ab300656 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300656 at 1/2000 dilution. Mouse IgG for IP (HRP) (ab131368) was used as the secondary at 1/5000 dilution. Lane 1(Input) : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg Lane 2(+) : HeLa whole cell lysate Lane 3(-) : Mouse monoclonal IgG2c (ab170191) instead of ab51841 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 24 seconds

All lanes:

Immunoprecipitation - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (<a href='/products/primary-antibodies/tata-binding-protein-tbp-antibody-mabcam51841-loading-control-ab300656'>ab300656</a>) at 1/30 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

HeLa whole cell lysate

Lane 3:

Immunoprecipitation - Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (<a href='/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a>)

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

Observed band size: 35 kDa,38 kDa

false

Exposure time: 24s

Supplier Data

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

This data was developed using ab300656, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on mouse stomach. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Supplier Data

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

This data was developed using ab300656, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on rat colon. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Lab

Immunoprecipitation - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

This data was developed using ab300656, the same antibody clone in a different buffer formulation. TATA binding protein TBP was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (10 µg) with ab300656 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300656 at 1/2000 dilution. Mouse IgG for IP (HRP) (ab131368) was used as the secondary at 1/5000 dilution. Lane 1(Input) : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 μg Lane 2(+) : NIH/3T3 whole cell lysate Lane 3(-) : Mouse monoclonal IgG2c (ab170191) instead of ab51841 in NIH/3T3 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 24 seconds

All lanes:

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

Lane 2:

NIH/3T3 whole cell lysate

Lane 3:

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

Predicted band size: 38 kDa

Observed band size: 36 kDa

false

Exposure time: 24s

Supplier Data

Western blot - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

All lanes:

Western blot - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (<a href='/products/primary-antibodies/tata-binding-protein-tbp-antibody-mabcam51841-loading-control-ab300656'>ab300656</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg

Lane 7:

Mouse testis tissue lysate at 20 µg

Lane 8:

Rat testis tissue lysate at 20 µg

Lane 9:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 40 kDa,35 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

mAbcam51841

アイソタイプ

IgG2c

キャリアフリー

Yes

交差種

Rat, Mouse, Human

アプリケーション

IP, IHC-P, ChIP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The TATA binding protein (TBP) also known as TBP or TFIID (Transcription Factor IID) is a central player in transcription initiation. This protein weighing approximately 38 kDa binds to the TATA box a DNA sequence found in the promoter region of many genes especially those transcribed by RNA polymerase II. TBP facilitates the loading of the transcription machinery onto DNA enabling the start of transcription. It is found inside the nucleus of eukaryotic cells present across diverse cell types owing to its role in gene expression.

Biological function summary

TBP serves as a pivotal anchor in the assembly of the transcription preinitiation complex contributing to the process by bending DNA to assist other transcription factors such as TFIIA and TFIIB in binding. Its role in this complex illustrates its importance in regulating gene expression at a fundamental level. TBP’s interaction with other transcription factors ensures precise transcription regulation reflecting its significant contribution to cellular activities.

Pathways

TBP acts within the RNA polymerase II transcription initiation pathway and is integral in regulating gene expression. TBP's involvement is critical for the process of starting the transcription of mRNA. In its pathway TBP interacts with proteins like TFIID helping to anchor other transcription factors in the complex. By collaborating with these proteins TBP shapes gene regulatory networks essential for a wide range of cellular functions.

TBP is linked to conditions such as spinocerebellar ataxia type 17 (SCA17) and Huntington's disease. SCA17 is associated with mutations in the TBP gene that disrupt its usual functioning leading to neurodegeneration. In Huntington's disease TBP's interaction with proteins such as huntingtin may influence nuclear signaling pathways involved in disease progression. The understanding of TBP’s role in these disorders underlines its relevance in research focused on genetic diseases and transcription-related pathologies.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 2194289, PubMed : 2363050, PubMed : 2374612, PubMed : 27193682, PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 27007846, PubMed : 33795473). The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed : 33795473). TBP forms the TFIID-A module together with TAF3 and TAF5 (PubMed : 33795473). TBP is a general transcription factor that functions at the core of the TFIID complex (PubMed : 2194289, PubMed : 2363050, PubMed : 2374612, PubMed : 27193682, PubMed : 33795473, PubMed : 9836642). During assembly of the core PIC on the promoter, as part of TFIID, TBP binds to and also bends promoter DNA, irrespective of whether the promoter contains a TATA box (PubMed : 33795473). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed : 26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription (PubMed : 15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter (PubMed : 15970593). SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA (PubMed : 15970593).

See full target information TBP

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TATA binding protein TBP 抗体 [mAbcam51841] (BSA and Azide free)

Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free)

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

Immunoprecipitation - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

Immunoprecipitation - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

Western blot - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise