Anti-TATA binding protein TBP 抗体 (ab28175)
Key features and details
- Rabbit polyclonal to TATA binding protein TBP
- Suitable for: ChIP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TATA binding protein TBP antibody
TATA binding protein TBP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TATA binding protein TBP -
由来種
Rabbit -
特異性
Ab28175 recognises TATA binding protein TBP. -
アプリケーション
適用あり: ChIPmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length TATA binding protein TBP (Human).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.9
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28175の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ChIP | (2) |
Use 5-10 µg for 25 µg of chromatin.
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特記事項 |
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ChIP
Use 5-10 µg for 25 µg of chromatin. |
ターゲット情報
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機能
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. -
組織特異性
Widely expressed, with levels highest in the testis and ovary. -
関連疾患
Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. -
配列類似性
Belongs to the TBP family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6908 Human
- Omim: 600075 Human
- SwissProt: P20226 Human
- Unigene: 590872 Human
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別名
- GTF2D antibody
- GTF2D1 antibody
- HDL4 antibody
see all
画像
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ChIP - Anti-TATA binding protein TBP antibody (ab28175)This image is courtesy of an anonymous Abreview
ChIP analysis using ab28175 binding TATA binding protein TBP in mouse liver nuclear tissue lysate. Cells were cross-linked for 10 minutes with 1% paraformaldehyde. Samples were incubated with primary antibody for 16 hours at 4°C in TE pH8, 150mM NaCl, Trition 1%, SDS 0,1%. Protein binding was detected using real-time PCR.
Negative Control: beads. -
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 8µg of ab28175 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (19)
ab28175 は 19 報の論文で使用されています。
- Wang J et al. TFIIB-related factor 1 is a nucleolar protein that promotes RNA polymerase I-directed transcription and tumour cell growth. Hum Mol Genet 32:104-121 (2023). PubMed: 35925837
- Biswas S et al. PEITC: A resounding molecule averts metastasis in breast cancer cells in vitro by regulating PKCδ/Aurora A interplay. Heliyon 8:e11656 (2022). PubMed: 36458309
- Lu N et al. miRNA-490-3p promotes the metastatic progression of invasive ductal carcinoma. Oncol Rep 45:706-716 (2021). PubMed: 33416185
- Mylonas C et al. A dual role for H2A.Z.1 in modulating the dynamics of RNA polymerase II initiation and elongation. Nat Struct Mol Biol 28:435-442 (2021). PubMed: 33972784
- Hsu SH et al. Crosstalk between E-Cadherin/β-Catenin and NF-κB Signaling Pathways: The Regulation of Host-Pathogen Interaction during Leptospirosis. Int J Mol Sci 22:N/A (2021). PubMed: 34884937