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AB818

Anti-TATA binding protein TBP 抗体 [1TBP18] - ChIP Grade

Anti-TATA binding protein TBP antibody [1TBP18] - Loading Control and ChIP Grade

4

(39 Reviews)

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(285 Publications)

Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818) is a mouse monoclonal antibody detecting TATA binding protein TBP in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF, ELISA, EM, ChIP. Suitable for Human, Mouse, Rat.

- Over 260 publications
- Trusted since 2002

別名を表示する

GTF2D1, TF2D, TFIID, TBP, TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit

5 Images
ChIP - Anti-TATA binding protein TBP antibody [1TBP18] - Loading Control and ChIP Grade (AB818)
  • ChIP

Lab

ChIP - Anti-TATA binding protein TBP antibody [1TBP18] - Loading Control and ChIP Grade (AB818)

Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 μg of chromatin, 8 μg of ab818 (blue), and 20 μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The last wash was performed with final wash buffer containing 250 mM NaCl. The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located in the core promoter region of the genes.

This image was generated using the ascites version of the product.

Flow Cytometry - Anti-TATA binding protein TBP antibody [1TBP18] - Loading Control and ChIP Grade (AB818)
  • Flow Cyt

Lab

Flow Cytometry - Anti-TATA binding protein TBP antibody [1TBP18] - Loading Control and ChIP Grade (AB818)

Overlay histogram showing HeLa cells stained with ab818 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab818, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

Western blot - Anti-TATA binding protein TBP antibody [1TBP18] - Loading Control and ChIP Grade (AB818)
  • WB

CiteAb

Western blot - Anti-TATA binding protein TBP antibody [1TBP18] - Loading Control and ChIP Grade (AB818)

Western Blotting using Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade, ab818. Publication image from Gromak, N. et al., 2016, Nat Commun, 27725641. Legend direct from paper.

TBP is an effector in HRASV12-induced replication stress.(a) TBP mRNA quantification by quantitative reverse transcriptase–PCR in BJ-HRASV12 cells 72 h after RAS induction. TBP mRNA levels were normalized to GAPDH and control. (b) Protein levels of TBP and β-ACTIN after RAS induction for the times indicated. (c) Densitometry quantification of TBP levels based on western blotting as in b after RAS induction for the times indicated. Values were normalized to 72 h control. N=3 (24 and 48 h), N=6 (72 h). (d) Twenty-four hours after RAS induction, cells were transfected with TBP siRNA (TBPsi #1) or control siRNA (nonTsi). Cells were processed for DNA fibre analysis or western blotting 48 h later and for 53BP1 staining 24 h later. (e) Protein levels of TBP, HRAS and GAPDH (loading control) 72 h after RAS induction and 48 h after siRNA transfection. (f) Quantification of nascent RNA synthesis by EU incorporation ±TBPsi #1 72 h after RAS induction. N=3. (g) Distribution of replication fork speeds ±TBPsi #1 72 h after RAS induction. N=3. (h) Median replication fork speeds ±TBPsi #1 72 h after RAS induction. N=3. (i) Percentages of cells containing more than eight 53BP1 foci, ±TBPsi #1 96 h after RAS induction. N=3. (j) Median replication fork speeds in cells treated with TBPsi #1 and DRB 72 h after RAS induction, compared with TBPsi #1 or DRB alone. N=3. (k) Percentages of cells treated with TBPsi #1 and DRB containing more than eight 53BP1 foci after 96 h after RAS induction, compared with TBPsi #1 or DRB alone. N=3. (l) Model for the role of TBP in HRASV12-induced replication stress. Means ±s.e.m. (bars) are shown. Student's t-test, *P<0.05, **P<0.01 and ***P<0.001.

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Western blot - Anti-TATA binding protein TBP antibody [1TBP18] - Loading Control and ChIP Grade (AB818)
  • WB

CiteAb

Western blot - Anti-TATA binding protein TBP antibody [1TBP18] - Loading Control and ChIP Grade (AB818)

Western Blotting using Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade, ab818. Publication image from Gromak, N. et al., 2016, Nat Commun, 27725641. Legend direct from paper.

TBP is an effector in HRASV12-induced replication stress.(a) TBP mRNA quantification by quantitative reverse transcriptase–PCR in BJ-HRASV12 cells 72 h after RAS induction. TBP mRNA levels were normalized to GAPDH and control. (b) Protein levels of TBP and β-ACTIN after RAS induction for the times indicated. (c) Densitometry quantification of TBP levels based on western blotting as in b after RAS induction for the times indicated. Values were normalized to 72 h control. N=3 (24 and 48 h), N=6 (72 h). (d) Twenty-four hours after RAS induction, cells were transfected with TBP siRNA (TBPsi #1) or control siRNA (nonTsi). Cells were processed for DNA fibre analysis or western blotting 48 h later and for 53BP1 staining 24 h later. (e) Protein levels of TBP, HRAS and GAPDH (loading control) 72 h after RAS induction and 48 h after siRNA transfection. (f) Quantification of nascent RNA synthesis by EU incorporation ±TBPsi #1 72 h after RAS induction. N=3. (g) Distribution of replication fork speeds ±TBPsi #1 72 h after RAS induction. N=3. (h) Median replication fork speeds ±TBPsi #1 72 h after RAS induction. N=3. (i) Percentages of cells containing more than eight 53BP1 foci, ±TBPsi #1 96 h after RAS induction. N=3. (j) Median replication fork speeds in cells treated with TBPsi #1 and DRB 72 h after RAS induction, compared with TBPsi #1 or DRB alone. N=3. (k) Percentages of cells treated with TBPsi #1 and DRB containing more than eight 53BP1 foci after 96 h after RAS induction, compared with TBPsi #1 or DRB alone. N=3. (l) Model for the role of TBP in HRASV12-induced replication stress. Means ±s.e.m. (bars) are shown. Student's t-test, *P<0.05, **P<0.01 and ***P<0.001.

false

Western blot - Anti-TATA binding protein TBP antibody [1TBP18] - Loading Control and ChIP Grade (AB818)
  • WB

CiteAb

Western blot - Anti-TATA binding protein TBP antibody [1TBP18] - Loading Control and ChIP Grade (AB818)

Western Blotting using Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade, ab818. Publication image from Gromak, N. et al., 2016, Nat Commun, 27725641. Legend direct from paper.

TBP overexpression causes replication stress and senescence.(a) Protein levels of TBP, p53 and TUBULIN (loading control) in BJ-TBPind cells treated with doxycycline for 1–6 days, to induce TBP overexpression. No doxycycline was used as a control. (b) Nascent RNA synthesis as measured by EU incorporation after TBP induction for 3 days. Scale bars, 10 µm. (c). Quantification of nuclear EU intensity after TBP induction for 1–4 days. N=4 (con, day 1, 2 and 4), N=5 (day 3). (d) Median replication fork speeds in after TBP induction. N=2 (day 1, 2 and 4), N=4 (con, day 3). Asterisks compare with control. (e) Nascent RNA synthesis as measured by EU incorporation in cells treated with DRB or dimethylsulfoxide (DMSO) (control) for 100 min, 72 h after TBP induction. N=3. (f) Median replication fork speeds in BJ-TBPind cells treated with DRB 72 h after TBP induction. N=3. (g). Percentage of cells displaying more than eight 53BP1 foci or micronuclei after TBP induction. Right panel : representative images of cells with 53BP1 foci and micronuclei. Asterisks compare with control. N=2–7. (h) Cell cycle distribution of 53BP1-positive cells 96 h after TBP induction as determined by co-staining with Cyclin A. N=2. (i) Representative images and percentages of β-galactosidase staining after TBP induction for 1–6 days. Scale bars, 100 µm. (j) Model of how HRASV12 and other growth factor oncogenes such as epidermal growth factor receptor (EGFR) induce replication stress by increasing transcription through TBP and other transcription factors. Additional mechanisms, such as reactive oxygen species, may also contribute to HRASV12-induced DNA damage. Means ±s.e.m. (bars) are shown. Student's t-test, *P<0.05, **P<0.01 and ***P<0.001.

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Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

1TBP18

アイソタイプ

IgG1

キャリアフリー

No

交差種

Human, Mouse, Rat

アプリケーション

IP, WB, EMSA, ICC, ChIP/Chip, Flow Cyt, IHC-P, IHC-Fr, ChIP, ELISA, ICC/IF

applications

免疫原

Synthetic Peptide within Human TBP. The exact immunogen used to generate this antibody is proprietary information.

P20226

エピトープ

Within amino acid residues 1-20 of human, mouse and rat TBP.The specific epitope for this antibody is accessible when the C-terminal domain of TBP is removed or when TBP is complexed with DNA.

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Reactivity data

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Mouse monoclonal IgG1, are suitable for use as an isotype control with this antibody.</p><p>We recommend Goat Anti-Mouse IgG H&amp;L (DyLight<sup>®</sup> 488) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-dylight-488-preadsorbed-ab96879'>ab96879</a>) secondary antibody</p>", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>We recommend Rabbit Anti-Mouse IgG H&;L (Alkaline Phosphatase) (<a href='/products/secondary-antibodies/rabbit-mouse-igg-h-l-alkaline-phosphatase-ab6729'>ab6729</a>) secondary antibody.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "EMSA-species-checked": "notRecommended", "EMSA-species-dilution-info": "", "EMSA-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Saccharomyces cerevisiae": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ChIPChip-species-checked": "notRecommended", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "<p></p>", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>We recommend Rabbit Anti-Mouse IgG H&;L (Alkaline Phosphatase) (<a href='/products/secondary-antibodies/rabbit-mouse-igg-h-l-alkaline-phosphatase-ab6729'>ab6729</a>) secondary antibody.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "EMSA-species-checked": "notRecommended", "EMSA-species-dilution-info": "", "EMSA-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Silk worm": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ChIPChip-species-checked": "notRecommended", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "<p></p>", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>We recommend Rabbit Anti-Mouse IgG H&;L (Alkaline Phosphatase) (<a href='/products/secondary-antibodies/rabbit-mouse-igg-h-l-alkaline-phosphatase-ab6729'>ab6729</a>) secondary antibody.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "EMSA-species-checked": "notRecommended", "EMSA-species-dilution-info": "", "EMSA-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Xenopus laevis": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ChIPChip-species-checked": "notRecommended", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "<p></p>", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-6 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> and <a href='/products/primary-antibodies/mouse-igg1-ct6-isotype-control-ab81216'>ab81216</a> - Mouse monoclonal IgG1, are suitable for use as an isotype control with this antibody.</p><p>We recommend Goat Anti-Mouse IgG H&amp;L (DyLight<sup>®</sup> 488) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-dylight-488-preadsorbed-ab96879'>ab96879</a>) secondary antibody</p>", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>We recommend Rabbit Anti-Mouse IgG H&;L (Alkaline Phosphatase) (<a href='/products/secondary-antibodies/rabbit-mouse-igg-h-l-alkaline-phosphatase-ab6729'>ab6729</a>) secondary antibody.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "EMSA-species-checked": "notRecommended", "EMSA-species-dilution-info": "", "EMSA-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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製品の詳細

What is this antibody validated in?
Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Electron microscopy (EM), ChIP in Human, Mouse, Rat samples.

What is the molecular weight of TATA binding protein TBP?
Anti-TATA binding protein TBP [1TBP18] - ChIP Grade (ab818) specifically detects a band for TATA binding protein TBP (UniProt: P20226) at a molecular weight of 38kDa.

Trusted by the scientific community
Anti-TATA binding protein TBP [1TBP18] - ChIP Grade (ab818) was first used in a scientific publication in 2002 and has been cited over 260 times in peer-reviewed journals.

Reviewed by scientists
Anti-TATA binding protein TBP [1TBP18] - ChIP Grade (ab818) has over 35 independent reviews from customers.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
精製に関する特記事項
Purified from TCS
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The TATA binding protein (TBP) also known as TBP or TFIID (Transcription Factor IID) is a central player in transcription initiation. This protein weighing approximately 38 kDa binds to the TATA box a DNA sequence found in the promoter region of many genes especially those transcribed by RNA polymerase II. TBP facilitates the loading of the transcription machinery onto DNA enabling the start of transcription. It is found inside the nucleus of eukaryotic cells present across diverse cell types owing to its role in gene expression.
Biological function summary

TBP serves as a pivotal anchor in the assembly of the transcription preinitiation complex contributing to the process by bending DNA to assist other transcription factors such as TFIIA and TFIIB in binding. Its role in this complex illustrates its importance in regulating gene expression at a fundamental level. TBP’s interaction with other transcription factors ensures precise transcription regulation reflecting its significant contribution to cellular activities.

Pathways

TBP acts within the RNA polymerase II transcription initiation pathway and is integral in regulating gene expression. TBP's involvement is critical for the process of starting the transcription of mRNA. In its pathway TBP interacts with proteins like TFIID helping to anchor other transcription factors in the complex. By collaborating with these proteins TBP shapes gene regulatory networks essential for a wide range of cellular functions.

TBP is linked to conditions such as spinocerebellar ataxia type 17 (SCA17) and Huntington's disease. SCA17 is associated with mutations in the TBP gene that disrupt its usual functioning leading to neurodegeneration. In Huntington's disease TBP's interaction with proteins such as huntingtin may influence nuclear signaling pathways involved in disease progression. The understanding of TBP’s role in these disorders underlines its relevance in research focused on genetic diseases and transcription-related pathologies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 2194289, PubMed : 2363050, PubMed : 2374612, PubMed : 27193682, PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 27007846, PubMed : 33795473). The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed : 33795473). TBP forms the TFIID-A module together with TAF3 and TAF5 (PubMed : 33795473). TBP is a general transcription factor that functions at the core of the TFIID complex (PubMed : 2194289, PubMed : 2363050, PubMed : 2374612, PubMed : 27193682, PubMed : 33795473, PubMed : 9836642). During assembly of the core PIC on the promoter, as part of TFIID, TBP binds to and also bends promoter DNA, irrespective of whether the promoter contains a TATA box (PubMed : 33795473). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed : 26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription (PubMed : 15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter (PubMed : 15970593). SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA (PubMed : 15970593).
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代替製品

Primary Antibodies

AB220788

Anti-TATA binding protein TBP antibody [EPR21954] - Loading Control and ChIP Grade

Recombinant|Advanced Validation|RabMAb|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-TATA binding protein TBP antibody [EPR21954] - Loading Control and ChIP Grade (AB220788)

  • 0
  • 0
Primary Antibodies

AB63766

Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (AB63766)

  • 5
  • 7
Primary Antibodies

AB237785

Anti-TATA binding protein TBP antibody [EPR21954] - ChIP Grade - BSA and Azide free

RabMAb|Advanced Validation|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TATA binding protein TBP antibody [EPR21954] - ChIP Grade - BSA and Azide free (AB237785)

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組合せ製品

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB16048

Anti-Lamin B1 antibody - Nuclear Envelope Marker

BOND RX™ Validated|KO Validated|Lab Essentials

Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB16048)

  • 5
  • 110
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
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