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AB109404

Anti-TAK1 (phospho S439) 抗体 [EPR2863]

Anti-TAK1 (phospho S439) antibody [EPR2863]

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(16 Publications)

Rabbit Recombinant Monoclonal TAK1 phospho S439 antibody. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 16 publications.

別名を表示する

TAK1, MAP3K7, Mitogen-activated protein kinase kinase kinase 7, Transforming growth factor-beta-activated kinase 1, TGF-beta-activated kinase 1

4 Images
Western blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)
  • WB

Unknown

Western blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)

All lanes:

Western blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HeLa cell lysate treated with transforming growth factor-beta (TGF-beta) at 10 µg

Predicted band size: 67 kDa

Observed band size: 75 kDa

false

Western blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)
  • WB

Lab

Western blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)

Dilution and blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 10 µg

Lane 2:

HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1β for 10 minutes whole cell lysate at 10 µg

Lane 3:

HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1β for 10 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase. at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 67 kDa

Observed band size: 75 kDa

false

Exposure time: 10s

Immunoprecipitation - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)
  • IP

Unknown

Immunoprecipitation - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)

Purified ab109404 at 1/50 dilution (2μg) immunoprecipitating TAK1 in HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.

Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate 10μg
Lane 2 (+) : ab109404 + HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab239974 in HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.

VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 75 kDa

All lanes:

Immunoprecipitation - Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404)

Predicted band size: 67 kDa

Observed band size: 80 kDa

false

Dot Blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)
  • Dot

Lab

Dot Blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)

Dot Blot analysis of Lane 1 : TAK1 (phospho S439) phospho peptide and Lane 2 : TAK1 non-phospho peptide labeling TAK1 (phospho S439) with ab109404 at 1/1000 dilution (0.009 μg/ml). 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100,000 dilution. Exposure time : 3 minutes.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR2863

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IP, WB, Dot

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This antibody only detects TAK1 phosphorylated at Serine 439 in Human or TAK1 phosphorylated at Serine 412 in Mouse.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TAK1 also known as MAP3K7 (Mitogen-activated protein kinase kinase kinase 7) is an important protein kinase that weighs approximately 63 kDa. This protein is expressed in various tissues and cells including HEK 293T cells. TAK1 plays a mechanical role as a part of the MAPK signaling cascade. It phosphorylates and activates downstream kinases which is key to transmitting cellular signals that regulate responses to external stimuli like cytokines and stress.
Biological function summary

TAK1 is involved in several cellular processes necessary for maintaining balance and responding to stress. TAK1 forms a complex with proteins like TAB1 TAB2 and TAB3 which are important for its activation and signaling function. This complex facilitates TAK1's involvement in inflammation and immune response indicating its significance in mediating cellular survival and apoptosis.

Pathways

TAK1 operates within the NF-κB and MAPK pathways two critical routes for cellular response to inflammation and stress. In the NF-κB pathway TAK1 activates IKK which triggers the degradation of IκB freeing NF-κB to move into the nucleus and activate transcription. In the MAPK pathway TAK1 directly influences JNK and p38 cascades revealing regulatory connections to proteins like MEK and ERK.

TAK1 has been linked to conditions such as cancer and inflammatory diseases. In cancers aberrant TAK1 activity can lead to enhanced cell proliferation and survival. Moreover inflammation-related disorders can arise from malfunctioning TAK1 signaling demonstrating its connection to proteins like TNF receptor-associated factors which are involved in inflammatory responses. Understanding TAK1's role in these disorders may pave the way for developing potential therapeutic interventions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed : 10094049, PubMed : 11460167, PubMed : 12589052, PubMed : 16845370, PubMed : 16893890, PubMed : 21512573, PubMed : 8663074, PubMed : 9079627). Plays an important role in the cascades of cellular responses evoked by changes in the environment (PubMed : 10094049, PubMed : 11460167, PubMed : 12589052, PubMed : 16845370, PubMed : 16893890, PubMed : 21512573, PubMed : 8663074, PubMed : 9079627). Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR) (PubMed : 16893890, PubMed : 9079627). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7 (PubMed : 11460167, PubMed : 8663074). These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs); both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1) (PubMed : 11460167, PubMed : 12589052, PubMed : 8663074). Independently of MAP2Ks and p38 MAPKs, acts as a key activator of NF-kappa-B by promoting activation of the I-kappa-B-kinase (IKK) core complex (PubMed : 12589052, PubMed : 8663074). Mechanistically, recruited to polyubiquitin chains of RIPK2 and IKBKG/NEMO via TAB2/MAP3K7IP2 and TAB3/MAP3K7IP3, and catalyzes phosphorylation and activation of IKBKB/IKKB component of the IKK complex, leading to NF-kappa-B activation (PubMed : 10094049, PubMed : 11460167). In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B (PubMed : 16893890). Promotes TRIM5 capsid-specific restriction activity (PubMed : 21512573). Phosphorylates RIPK1 at 'Ser-321' which positively regulates RIPK1 interaction with RIPK3 to promote necroptosis but negatively regulates RIPK1 kinase activity and its interaction with FADD to mediate apoptosis (By similarity). Phosphorylates STING1 in response to cGAMP-activation, promoting association between STEEP1 and STING1 and STING1 translocation to COPII vesicles (PubMed : 37832545).
See full target information MAP3K7 phospho S439
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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:21106 PubMed38036641

2023

Application of human platelet lysate in chondrocyte expansion promotes chondrogenic phenotype and slows senescence progression via BMP-TAK1-p38 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Narong Chitchongyingcharoen,Tulyapruek Tawonsawatruk,Jitrada Phetfong,Wrattya Aroontanee,Aungkura Supokawej

Communications biology 5:186 PubMed35233032

2022

H5N1 infection impairs the alveolar epithelial barrier through intercellular junction proteins via Itch-mediated proteasomal degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Ruan,Yuling Sun,Jingting Zhang,Jing Sun,Wei Liu,Richard A Prinz,Daxin Peng,Xiufan Liu,Xiulong Xu

Proceedings of the National Academy of Sciences of the United States of America 119:e2115669119 PubMed35238667

2022

Molecular mechanism underlying the TLR4 antagonistic and antiseptic activities of papiliocin, an insect innate immune response molecule.

Applications

Unspecified application

Species

Unspecified reactive species

Manigandan Krishnan,Joonhyeok Choi,Ahjin Jang,Sungjae Choi,Jiwon Yeon,Mihee Jang,Yeongjoon Lee,Kkabi Son,Soon Young Shin,Myeong Seon Jeong,Yangmee Kim

Cellular and molecular gastroenterology and hepatology 12:1121-1143 PubMed33962073

2021

TAK1 Is a Novel Target in Hepatocellular Carcinoma and Contributes to Sorafenib Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Shunjie Xia,Lin Ji,Liye Tao,Yu Pan,Zhongjie Lin,Zhe Wan,Haoqi Pan,Jie Zhao,Liuxin Cai,Junjie Xu,Xiujun Cai

Communications biology 3:327 PubMed32581266

2020

Farnesoid X receptor activation inhibits TGFBR1/TAK1-mediated vascular inflammation and calcification via miR-135a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Li,Shijun Zhang,Xiaoqing Chen,Jingkang Ji,Wenqing Yang,Ting Gui,Zhibo Gai,Yunlun Li

American journal of cancer research 10:454-472 PubMed32195020

2020

TLR4 activation leads to anti-EGFR therapy resistance in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Houyu Ju,Zhenrong Hu,Yusheng Lu,Yunteng Wu,Liming Zhang,Dongliang Wei,Wei Guo,Weiya Xia,Shuli Liu,Guoxin Ren,Jingzhou Hu

Archives of pharmacal research 42:1063-1070 PubMed31802426

2019

Hesperetin ameliorates lipopolysaccharide-induced acute lung injury in mice through regulating the TLR4-MyD88-NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Naigang Wang,Cuiping Geng,Haiyun Sun,Xia Wang,Fangmin Li,Xunchao Liu

Journal of cellular biochemistry 120:19764-19774 PubMed31297873

2019

Notoginsenoside R1 protects WI-38 cells against lipopolysaccharide-triggered injury via adjusting the miR-181a/TLR4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Daolin Qian,Xiankun Shao,Yingchun Li,Xinyan Sun

Frontiers in pharmacology 9:1538 PubMed30713497

2019

20(S)-Protopanaxadiol Inhibits Titanium Particle-Induced Inflammatory Osteolysis and RANKL-Mediated Osteoclastogenesis via MAPK and NF-κB Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Chenhao Pan,Haojie Shan,Tianyi Wu,Wei Liu,Yiwei Lin,Wenyang Xia,Feng Wang,Zubin Zhou,Xiaowei Yu

EMBO reports 19: PubMed29674348

2018

RKIP mediates autoimmune inflammation by positively regulating IL-17R signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wenlong Lin,Ning Wang,Kangxing Zhou,Fasheng Su,Yu Jiang,Jianan Shou,Huan Liu,Chunmei Ma,Youchun Qian,Kai Wang,Xiaojian Wang
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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