AB307139

Anti-TAC1 抗体 [EPR26567-18-1-3] - BSA and Azide free

Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
Advanced Validation
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Rabbit Recombinant Monoclonal Protachykinin-1 antibody. Carrier free. Suitable for IHC-P, mIHC and reacts with Human, Rat, Mouse samples.

別名を表示する

NKA, NKNA, TAC2, TAC1, Protachykinin-1, PPT

6 Images

Supplier Data

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)

This data was developed using ab307138, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human spinal cord tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human spinal cord. The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Supplier Data

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)

This data was developed using ab307138, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : No staining on mouse liver. The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Supplier Data

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)

This data was developed using ab307138, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining predominanlty on dorsal horns of mouse spinal cord. The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Supplier Data

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)

This data was developed using ab307138, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining predominanlty on dorsal horns of rat spinal cord. The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)

This data was developed using ab307138, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat spinal cord tissue section labelling GPCR GPR17 with ab314307 at 1/2000 dilution (B), TAC1 with ab307138 at 1/500 dilution (C), and ARMET/ARP with ab316935 at 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-GPCR GPR17 (magenta; Opal™ 690), anti-TAC1 (green; Opal™ 520) and anti-ARMET/ARP (yellow; Opal™ 570) on rat spinal cord.
Panel B : anti-GPCR GPR17 staining oligodendrocytes in rat spinal cord.
Panel C : anti-TAC1 staining posterior horns (sensory) in rat spinal cord.
Panel D : anti-ARMET/ARP staining neurons in rat spinal cord.

The section was incubated in three rounds of staining : in the order of ab314307, ab307138 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)

This data was developed using ab307138, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue section labelling GPCR GPR17 with ab314307 at 1/2000 dilution (B), TAC1 with ab307138 at 1/500 dilution (C), and ARMET/ARP with ab316935 at 1/20000 (D). Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-GPCR GPR17 (magenta; Opal™ 690), anti-TAC1 (green; Opal™ 520) and anti-ARMET/ARP (yellow; Opal™ 570) on mouse spinal cord.
Panel B : anti-GPCR GPR17 staining oligodendrocytes in mouse spinal cord.
Panel C : anti-TAC1 staining posterior horns (sensory) in mouse spinal cord.
Panel D : anti-ARMET/ARP staining neurons in mouse spinal cord.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR26567-18-1-3

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Rat, Human

アプリケーション

mIHC, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TAC1 also known as Tachykinin Precursor 1 or Substance P is a gene that encodes a peptide which functions mechanically in neuropeptide signaling. TAC1 polypeptide has a mass of approximately 15 kDa. It is highly expressed in cells of the nervous system including the central and peripheral systems. The peptide derived from TAC1 participates in neurotransmission and modulates inflammatory responses.

Biological function summary

TAC1 influences multiple processes primarily through interaction with the neurokinin receptors. The peptide is not part of a larger protein complex but it exerts its effects by binding to the tachykinin receptor NK1 which plays a role in regulating pain responses mood anxiety and the emetic reflex. TAC1-derived peptides are involved in promoting vasodilation and bronchoconstriction impacting cardiovascular and respiratory systems.

Pathways

TAC1 acts in the tachykinin signaling pathway and affects various physiological processes. It is critical for the neurokinin pathway impacting the transmission of stress signals. TAC1 interacts closely with proteins like neurokinin-1 receptor (NK1R) and is involved in downstream effects such as calcium signaling pathways. These pathways highlight TAC1's role in communicating pain and stress stimuli across neurons.

TAC1 relates strongly to chronic pain and inflammatory diseases. Abnormal TAC1 expression has been linked to fibromyalgia and inflammatory bowel disease. In these conditions TAC1 peptides engage neuroinflammatory pathways often in association with other neuropeptides like CGRP (calcitonin gene-related peptide) leading to elevated inflammatory states. Understanding TAC1 interactions helps in strategizing therapeutic approaches for these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.. Substance P. Is a ligand for TACR1, and triggers G protein-coupled receptor signaling via activation of phosphatidylinositol hydrolysis by phospholipase C. Substance P binding to TACR1 also triggers signaling via activation of adenylate cyclase activity which results in increased intracellular levels of cyclic AMP (cAMP) (By similarity). Is also a TACR3 agonist with low receptor affinity (PubMed : 37391393). Basic secretagogue neuropeptide released from the terminals of specific sensory nerves (PubMed : 30686732). Acts as a ligand for MRGPRX2 receptor in mast cells, initiating a signaling that mediates neurogenic inflammation and pain (PubMed : 30686732, PubMed : 34789875). Neurogenic inflammation includes mast cell activation, recruitment of immune cells and release of inflammatory mediators, such as cytokines and chemokines (By similarity). The inflammatory response can then activate or sensitize nociceptors, promoting pain (By similarity).. Neurokinin A. Is a ligand for TACR2, and triggers G protein-coupled receptor signaling via activation of G(q) and phosphatidylinositol hydrolysis by phospholipase C (PubMed : 35882833). Binding to TACR2 also triggers signaling via activation of adenylate cyclase activity which results in increased intracellular levels of cyclic AMP (cAMP).

See full target information TAC1

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TAC1 抗体 [EPR26567-18-1-3] - BSA and Azide free

Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)

Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)

Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise