Anti-T-bet / Tbx21 抗体 [EPR9302] (ab154200)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)](https://www.abcam.co.jp/ps/products/154/ab154200/Images/ab154200-318896-anti-t-bet-tbx21-antibody-epr9302-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9302] to T-bet / Tbx21
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-T-bet / Tbx21 antibody [EPR9302]
T-bet / Tbx21 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9302] to T-bet / Tbx21 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details
適用なし: ICC/IF,IP or WB -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human T-bet/ Tbx21. The exact sequence is proprietary.
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ポジティブ･コントロール
- IHC-P: Hodgkin's Lymphoma tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9302 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154200の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (4) |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
追加情報
Is unsuitable for ICC/IF,IP or WB.
ターゲット情報
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機能
Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. -
組織特異性
T-cell specific. -
関連疾患
Genetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities. -
配列類似性
Contains 1 T-box DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 30009 Human
- Omim: 604895 Human
- SwissProt: Q9UL17 Human
- Unigene: 272409 Human
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別名
- T bet antibody
- T box 21 antibody
- T box expressed in T cells antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin lymphoma tissue sections labeling T-bet / Tbx21 with Purified ab154200 at 1:500 dilution (0.68 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)](https://www.abcam.co.jp/ps/products/154/ab154200/Images/ab154200-160189-anti-t-bet-tbx21-antibody-epr9302-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma labeling T-bet/Tbx21 with ab154200 at 1/250 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)](https://www.abcam.co.jp/ps/products/154/ab154200/Images/ab154200-4-benefits-of-recombinant-antibodies.png)
Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154200 は 1 報の論文で使用されています。
- Brune MM et al. Effects of lenalidomide on the bone marrow microenvironment in acute myeloid leukemia: Translational analysis of the HOVON103 AML/SAKK30/10 Swiss trial cohort. Ann Hematol 100:1169-1179 (2021). PubMed: 33704530