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リコンビナントRabMAb

Anti-T-bet / Tbx21 抗体 [EPR25086-9] - BSA and Azide free (ab300452)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)
  • Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25086-9] to T-bet / Tbx21 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free
    T-bet / Tbx21 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25086-9] to T-bet / Tbx21 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IP or WB

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Mouse spleen, Mouse thymus, Rat spleen FFPE tissues.

  • 特記事項

    ab300452 is the carrier-free version of ab300451.

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300452の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IP or WB.

ターゲット情報

  • 機能

    Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs.

  • 組織特異性

    T-cell specific.

  • 関連疾患

    Genetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities.

  • 配列類似性

    Contains 1 T-box DNA-binding domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • T bet antibody
      • T box 21 antibody
      • T box expressed in T cells antibody
      • T box protein 21 antibody
      • T box transcription factor TBX21 antibody
      • T cell specific T box transcription factor antibody
      • T cell specific T box transcription factor T bet antibody
      • T PET antibody
      • T-box protein 21 antibody
      • T-box transcription factor TBX21 antibody
      • T-cell-specific T-box transcription factor T-bet antibody
      • TBET antibody
      • TBLYM antibody
      • TBX 21 antibody
      • Tbx21 antibody
      • TBX21_HUMAN antibody
      • TPET antibody
      • Transcription factor TBLYM antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)

      This data was developed using ab300451, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse brain labeling T-bet / Tbx21 with ab300451 at 1/6000 dilution (0.093 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Negative control: no staining on mouse brain (PMID:10761931). The section was incubated with ab300451 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)

      This data was developed using ab300451, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse spleen labeling T-bet / Tbx21 with ab300451 at 1/6000 dilution (0.093 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Nuclear staining on mouse spleen. The section was incubated with ab300451 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)

      This data was developed using ab300451, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse thymus labeling T-bet / Tbx21 with ab300451 at 1/6000 dilution (0.093 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Nuclear staining on mouse thymus. The section was incubated with ab300451 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)

      This data was developed using ab300451, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat brain labeling T-bet / Tbx21 with ab300451 at 1/6000 dilution (0.093 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Negative control: no staining on rat brain (PMID:10761931). The section was incubated with ab300451 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)

      This data was developed using ab300451, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat spleen labeling T-bet / Tbx21 with ab300451 at 1/6000 dilution (0.093 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Nuclear staining on rat spleen. The section was incubated with ab300451 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)
      Anti-T-bet / Tbx21 antibody [EPR25086-9] - BSA and Azide free (AB300452)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300452 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300452 は論文での使用が確認できていません。

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