Anti-SYPL1 抗体 [EPR14317] (ab184176)
![Western blot - Anti-SYPL1 antibody [EPR14317] (ab184176)](https://www.abcam.co.jp/ps/products/184/ab184176/Images/ab184176-216373-anti-sypl1-antibody-epr14317-western-blot.jpg "Western blot - Anti-SYPL1 antibody [EPR14317] (ab184176)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14317] to SYPL1
- Suitable for: IHC-P, WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SYPL1 antibody [EPR14317]
SYPL1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14317] to SYPL1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human fetal liver, fetal lung, fetal kidney and adipose tissue lysates; Human ovarian carcinoma and colon tissues; HepG2 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14317 -
アイソタイプ
IgG
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184176の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/1000 - 1/10000. Detects a band of approximately 29-37 kDa (predicted molecular weight: 29 kDa).
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| Flow Cyt (Intra) |
1/130.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 29-37 kDa (predicted molecular weight: 29 kDa). |
|
Flow Cyt (Intra)
1/130. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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配列類似性
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain. -
細胞内局在
Cytoplasmic vesicle membrane. Melanosome. Cytoplasmic transport vesicles (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 6856 Human
- SwissProt: Q16563 Human
- Unigene: 80919 Human
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別名
- H-SP1 antibody
- Pantophysin antibody
- Synaptophysin-like protein 1 antibody
see all
画像
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Western blot - Anti-SYPL1 antibody [EPR14317] (ab184176)All lanes : Anti-SYPL1 antibody [EPR14317] (ab184176) at 1/5000 dilution
Lane 1 : Human fetal lung tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 29 kDa -
![Western blot - Anti-SYPL1 antibody [EPR14317] (ab184176)](https://www.abcam.co.jp/ps/products/184/ab184176/Images/ab184176-216372-anti-sypl1-antibody-epr14317-western-blot.jpg)
Western blot - Anti-SYPL1 antibody [EPR14317] (ab184176)All lanes : Anti-SYPL1 antibody [EPR14317] (ab184176) at 1/1000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human adipose tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 29 kDa -
![Flow Cytometry (Intracellular) - Anti-SYPL1 antibody [EPR14317] (ab184176)](https://www.abcam.co.jp/ps/products/184/ab184176/Images/ab184176-216377-anti-sypl1-antibody-epr14317-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SYPL1 antibody [EPR14317] (ab184176)Intracellular flow cytometric analysis of HepG2 cells (paraformaldehyde-fixed, 2%)labeling SYPL1 with ab184176 at 1/130 dilution (red) or a rabbit IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] (ab184176)](https://www.abcam.co.jp/ps/products/184/ab184176/Images/ab184176-216376-anti-sypl1-antibody-epr14317-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] (ab184176)Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SYPL1 with ab184176 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] (ab184176)](https://www.abcam.co.jp/ps/products/184/ab184176/Images/ab184176-216375-anti-sypl1-antibody-epr14317-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] (ab184176)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SYPL1 with ab184176 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-SYPL1 antibody [EPR14317] (ab184176)](https://www.abcam.co.jp/ps/products/184/ab184176/Images/ab184176-6-benefits-of-recombinant-antibodies.png)
Anti-SYPL1 antibody [EPR14317] (ab184176)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (7)
ab184176 は 7 報の論文で使用されています。
- Yin L et al. AR antagonists develop drug resistance through TOMM20 autophagic degradation-promoted transformation to neuroendocrine prostate cancer. J Exp Clin Cancer Res 42:204 (2023). PubMed: 37563661
- Liu J et al. SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility. Nat Commun 14:5113 (2023). PubMed: 37607933
- Situ J et al. Comparative Proteomic Analysis Reveals the Effect of Selenoprotein W Deficiency on Oligodendrogenesis in Fear Memory. Antioxidants (Basel) 11:N/A (2022). PubMed: 35624863
- Zhao L et al. Effects of Fibroblast Growth Factor 21 on Lactate Uptake and Usage in Mice with Diabetes-Associated Cognitive Decline. Mol Neurobiol 59:5656-5672 (2022). PubMed: 35761156
- Liu L et al. Serum SYPL1 is a promising diagnostic biomarker for colorectal cancer. Clin Chim Acta 509:36-42 (2020). PubMed: 32502495
- Song Y et al. SYPL1 Inhibits Apoptosis in Pancreatic Ductal Adenocarcinoma via Suppression of ROS-Induced ERK Activation. Front Oncol 10:1482 (2020). PubMed: 33042794
- Chen DH et al. SYPL1 overexpression predicts poor prognosis of hepatocellular carcinoma and associates with epithelial-mesenchymal transition. Oncol Rep 38:1533-1542 (2017). PubMed: 28731154