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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Syntaxin 4 抗体 [EPR15473] (ab184545)

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Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
  • Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
  • Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
  • Anti-Syntaxin 4 antibody [EPR15473] (ab184545)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15473] to Syntaxin 4
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Syntaxin 4 antibody [EPR15473]
    Syntaxin 4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15473] to Syntaxin 4

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HEK-293, K562, HeLa and A431 cell lysates. Wild-type HAP1 cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184545の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

ターゲット情報

  • 機能

    Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.

  • 組織特異性

    Expressed in neutrophils and neutrophil-differentiated HL-60 cells. Expression in neutrophils increases with differentiation.

  • 配列類似性

    Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • NY REN 31 antigen antibody
    • p35 2 antibody
    • PLACENTAL antibody
    • Renal carcinoma antigen NY REN 31 antibody
    • Renal carcinoma antigen NY-REN-31 antibody
    • Stx4 antibody
    • STX4_HUMAN antibody
    • STX4A antibody
    • Syntaxin 4 antibody
    • syntaxin 4A (placental) antibody
    • Syntaxin 4A antibody
    • Syntaxin-4 antibody
    see all

画像

  • Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
    Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Syntaxin 4 knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab184545 observed at 1X kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab184545 was shown to recognize Syntaxin 4 when Syntaxin 4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Syntaxin 4 knockout samples were subjected to SDS-PAGE. ab184545 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
    Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
    All lanes : Anti-Syntaxin 4 antibody [EPR15473] (ab184545) at 1/2000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
    Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
    Anti-Syntaxin 4 antibody [EPR15473] (ab184545) at 1/10000 dilution + A431 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
    Anti-Syntaxin 4 antibody [EPR15473] (ab184545)

参考文献 (5)

ab184545 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184545 は 5 報の論文で使用されています。

  • Yu Z  et al. Reduced oligodendrocyte exosome secretion in multiple system atrophy involves SNARE dysfunction. Brain 143:1780-1797 (2020). PubMed: 32428221
  • Kitao T  et al. Legionella Manipulates Non-canonical SNARE Pairing Using a Bacterial Deubiquitinase. Cell Rep 32:108107 (2020). PubMed: 32905772
  • Sikorski K  et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
  • Lizarbe B  et al. Neurochemical Modifications in the Hippocampus, Cortex and Hypothalamus of Mice Exposed to Long-Term High-Fat Diet. Front Neurosci 12:985 (2018). PubMed: 30670942
  • Machitani M  et al. MicroRNA miR-27 Inhibits Adenovirus Infection by Suppressing the Expression of SNAP25 and TXN2. J Virol 91:N/A (2017). WB ; Human . PubMed: 28356525

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