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AB6179

Anti-Synphilin 1 抗体

Anti-Synphilin 1 antibody

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(3 Publications)

Rabbit Polyclonal Synphilin 1 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Synphilin-1 aa 900 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

Synphilin-1, Sph1, Alpha-synuclein-interacting protein, SNCAIP

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synphilin 1 antibody (AB6179)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synphilin 1 antibody (AB6179)

IHC image of ab6179 staining in rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6179, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Rat

アプリケーション

IHC-P

applications

免疫原

Synthetic Peptide within Human Synphilin-1 aa 900 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6H5

特異性

Immunohistochemical analysis of human brain indicates a high level of specificity for this antiserum. Cross reactivity: This antibody is known to react with rat and human. Cross reactivity with other species has not been tested yet.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

This antibody stains Lewy bodies in Dorsal raphe nucleus of Parkinson's diseased human brain.
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出荷温度及び保存条件

製品の状態
Liquid
精製度
Whole antiserum
バッファー組成
pH: 7 - 7.4
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Add glycerol to a final volume of 50% for extra stability and aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Synphilin-1 also known as SNCAIP is a protein that plays a role in cellular mechanisms. It has a molecular mass of approximately 90 kDa. Synphilin-1 is expressed in various tissues with high levels seen in the brain particularly within neurons. This protein undergoes interactions with other cellular components and participates in the regulation of protein aggregation which is critical in maintaining cellular homeostasis.
Biological function summary

Synphilin-1 associates with alpha-synuclein to form complexes that influence neuronal function. It participates in the modulation of synaptic activity and neuronal signaling. The protein functions as a scaffold supporting various signaling molecules and influencing their activity. Synphilin-1's role in these processes highlights its importance in maintaining neural cell health and function.

Pathways

Synphilin-1 influences the ubiquitin-proteasome system that is responsible for protein degradation and turnover. This involvement links Synphilin-1 to the degradation of misfolded proteins a function that is shared with ubiquitin ligases. Another pathway of interest is the endoplasmic reticulum-associated degradation (ERAD) pathway where Synphilin-1 mediates the removal of aberrant proteins. Synphilin-1’s association with the ubiquitin-proteasome pathway and its interactions with related proteins like parkin provide insight into its regulatory functions.

Synphilin-1 exhibits a significant connection to neurodegenerative diseases such as Parkinson's disease. Its interaction with alpha-synuclein an important player in this disorder highlights its involvement in disease pathogenesis. Additionally Synphilin-1 has been linked to Alzheimer's disease where its aggregation-modulating properties may influence disease progression. These relationships make Synphilin-1 a target of interest for therapeutic research in the context of neurodegeneration.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1.
See full target information Synphilin-1
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Life sciences 221:35-46 PubMed30735733

2019

Panaxadiol inhibits synaptic dysfunction in Alzheimer's disease and targets the Fyn protein in APP/PS1 mice and APP-SH-SY5Y cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xicai Liang,Yingjia Yao,Ying Lin,Liang Kong,Honghe Xiao,Yue Shi,Jingxian Yang

Brain research. Developmental brain research 149:131-42 PubMed15063093

2004

Parkin expression in the developing mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Kati Kühn,Xin-Ran Zhu,Hermann Lübbert,Christine C Stichel

BMC neuroscience 2:11 PubMed11591219

2001

Deletion of the alpha-synuclein locus in a subpopulation of C57BL/6J inbred mice.

Applications

Unspecified application

Species

Unspecified reactive species

C G Specht,R Schoepfer
View all publications
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