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リコンビナントRabMAb

Anti-Synip 抗体 [EP302Y] (ab40674)

Submit a review Q&A (1)References (3)
Western blot - Anti-Synip antibody [EP302Y] (ab40674)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP302Y] to Synip
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Synip antibody [EP302Y]
    Synip 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP302Y] to Synip

  • 由来種

    Rabbit

  • 特異性

    This antibody is specific for Synip. It is predicted to detect the isoform 2 based an sequence analysis.

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human, Mouse, and Rat skeletal muscle lysates. IHC-P: Human lung cancer, Mouse and Rat stomach tissue.

  • 特記事項

    This product has switched from a hybridoma to recombinant production method on 13th September 2024.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab40674の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa).
IHC-P
1/1000 - 1/3500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa).
IHC-P
1/1000 - 1/3500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF or IP.

ターゲット情報

  • 機能

    Plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. Inhibits the translocation of SLC2A4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2. Stimulation with insulin disrupts the interaction with STX4A, leading to increased levels of SLC2A4 at the plasma membrane. May also play a role in the regulation of insulin release by pancreatic beta cells after stimulation by glucose.

  • 配列類似性

    Contains 1 PDZ (DHR) domain.
    Contains 1 WW domain.

  • 翻訳後修飾

    Phosphorylated on Ser-99 by PKB/AKT2 after insulin treatment. Phosphorylation on Ser-99 abolishes the interaction with STX4A (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • FLJ16496 antibody
    • MGC149829 antibody
    • MGC50337 antibody
    • OTTHUMP00000082667 antibody
    • OTTHUMP00000227400 antibody
    • STX4-interacting protein antibody
    • STXB4_HUMAN antibody
    • Stxbp4 antibody
    • Synip antibody
    • Syntaxin 4 interacting protein antibody
    • Syntaxin 4-interacting protein antibody
    • Syntaxin binding protein 4 antibody
    • Syntaxin-binding protein 4 antibody
    see all

画像

  • Western blot - Anti-Synip antibody [EP302Y] (ab40674)
    Western blot - Anti-Synip antibody [EP302Y] (ab40674)
    All lanes : Anti-Synip antibody [EP302Y] (ab40674) at 1/5000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Mouse skeletal muscle lysate
    Lane 3 : Rat skeletal muscle lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 61 kDa
    Observed band size: 68 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Synip with ab40674 at 1/1000 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Synip with ab40674 at 1/3500 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Synip with ab40674 at 1/1000 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (3)

ab40674 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab40674 は 3 報の論文で使用されています。

  • Otaka Y  et al. STXBP4 Drives Tumor Growth and Is Associated with Poor Prognosis through PDGF Receptor Signaling in Lung Squamous Cell Carcinoma. Clin Cancer Res 23:3442-3452 (2017). IHC . PubMed: 28087642
  • Taniai E  et al. Crosstalk between PTEN/Akt2 and TGFbeta signaling involving EGF receptor down-regulation during the tumor promotion process from the early stage in a rat two-stage hepatocarcinogenesis model. Cancer Sci 100:813-20 (2009). IHC-P ; Rat . PubMed: 19309364
  • Okada S  et al. Synip phosphorylation is required for insulin-stimulated Glut4 translocation. Biochem Biophys Res Commun 356:102-6 (2007). PubMed: 17336927

レビューと Q&A

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レビューを送る 質問を送る

Question

Hello, we are considering to purchase your TRAIL human ELISA Kit(Cat#: ab40674).
I want to detect the partial N-terminus(a.a114-281) of TRAIL by using the ELISA Kit.
But I wonder whether the Kit can detect the partial amino acid of TRAIL or not.
I have concerned about the TRAIL ELISA kit as it may have low specificity to detect parital TRAIL.
It's going to be helpful me with explanation if you don't have any above data I've mentioned.
Please reply to email asap since we are in hurry to carry out the experiment.
Thank you for your help.

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Answer

Thank you for contacting us.

The immunogen sequence of anti TRAIL antibody used in this kit corresponds to 114-281 of human TRAIL. It is more likely that it will detect your partial N-terminus (aa114-281) protein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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