Anti-Synaptotagmin 抗体 [ASV30] (ab13259)
Key features and details
- Mouse monoclonal [ASV30] to Synaptotagmin
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat
- Isotype: IgG2a
製品の概要
-
製品名
Anti-Synaptotagmin antibody [ASV30]
Synaptotagmin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [ASV30] to Synaptotagmin -
由来種
Mouse -
特異性
ab13259 is not specific to SYT2 but is a pan-synaptotagmin antibody. It does not distinguish between the Synaptotagmin family of proteins.
-
アプリケーション
適用あり: WB, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Full length protein corresponding to Rat Synaptotagmin. Rat brain synaptic junction protein complexes
Database link: P21707 -
ポジティブ・コントロール
- Mouse or Rat brain tissue extract.
-
特記事項
This product was changed from ascites to tissue culture supernatant on 5th July 2019. Lot numbers higher than GR3258922 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Protein G purified -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
ASV30 -
アイソタイプ
IgG2a -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13259の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000.
|
ICC/IF |
Use a concentration of 5 µg/ml.
|
|
IP |
Use a concentration of 5 µg/ml.
|
特記事項 |
---|
WB
1/1000. |
ICC/IF
Use a concentration of 5 µg/ml. |
IP
Use a concentration of 5 µg/ml. |
ターゲット情報
-
機能
May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. -
配列類似性
Belongs to the synaptotagmin family.
Contains 2 C2 domains. -
ドメイン
The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
The second C2 domain mediates interaction with Stonin 2. -
細胞内局在
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane. Synaptic vesicles and chromaffin granules. - Information by UniProt
-
参照データベース
- Entrez Gene: 20979 Mouse
- Entrez Gene: 20980 Mouse
- Entrez Gene: 24805 Rat
- Entrez Gene: 25716 Rat
- SwissProt: P46096 Mouse
- SwissProt: P46097 Mouse
- SwissProt: P21707 Rat
- SwissProt: P29101 Rat
see all -
別名
- HsT1192 antibody
- MYSPC antibody
- P65 antibody
see all
画像
-
All lanes : Anti-Synaptotagmin antibody [ASV30] (ab13259) at 1/1000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : Lysates prepared from mouse brain
Lane 3 : Lysates prepared from rat brainThis image was generated using the ascites version of the product.
-
ICC/IF image of ab13259 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13259, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
-
Synaptotagmin was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Mouse monoclonal to Synaptotagmin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13259.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 64kDa; SynaptotagminThis image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (23)
ab13259 は 23 報の論文で使用されています。
- Lu CW et al. Asiatic Acid Prevents Cognitive Deficits by Inhibiting Calpain Activation and Preserving Synaptic and Mitochondrial Function in Rats with Kainic Acid-Induced Seizure. Biomedicines 9:N/A (2021). PubMed: 33802221
- Ivanova D et al. Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes. Sci Adv 7:N/A (2021). PubMed: 33931449
- Yi LT et al. Berberine exerts antidepressant-like effects via regulating miR-34a-synaptotagmin1/Bcl-2 axis. Chin Herb Med 13:116-123 (2021). PubMed: 36117760
- Ramos H et al. Neuromodulation Induced by Sitagliptin: A New Strategy for Treating Diabetic Retinopathy. Biomedicines 9:N/A (2021). PubMed: 34944588
- Saini J et al. A Novel Bioengineered Functional Motor Unit Platform to Study Neuromuscular Interaction. J Clin Med 9:N/A (2020). PubMed: 33050427