Anti-Synaptotagmin 抗体 (ab51164)
Key features and details
- Rabbit polyclonal to Synaptotagmin
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Synaptotagmin antibody
Synaptotagmin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Synaptotagmin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ELISAmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthesized non-phosphopeptide derived from human Synaptotagmin around the phosphorylation site of serine 309.
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ポジティブ・コントロール
- 293 cell extract treated with PMA (125ng/ml, 30min) and human breast carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51164の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).
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IHC-P |
Use at an assay dependent concentration.
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ELISA |
1/20000.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa). |
IHC-P
Use at an assay dependent concentration. |
ELISA
1/20000. |
ターゲット情報
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機能
May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. -
配列類似性
Belongs to the synaptotagmin family.
Contains 2 C2 domains. -
ドメイン
The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
The second C2 domain mediates interaction with Stonin 2. -
細胞内局在
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane. Synaptic vesicles and chromaffin granules. - Information by UniProt
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参照データベース
- Entrez Gene: 127833 Human
- Entrez Gene: 6857 Human
- Entrez Gene: 6860 Human
- Entrez Gene: 6861 Human
- Entrez Gene: 84258 Human
- Entrez Gene: 20979 Mouse
- Entrez Gene: 20980 Mouse
- Omim: 185605 Human
see all -
別名
- HsT1192 antibody
- MYSPC antibody
- P65 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptotagmin antibody (ab51164)Immunohistochemical analysis of human breast carcinoma tissue (paraffin section) using ab51164 (1/50 dilution); with and without peptide.
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All lanes : Anti-Synaptotagmin antibody (ab51164) at 1/500 dilution
Lane 1 : 293 cell extract treated with
PMA (125ng/ml, 30min); without peptide
Lane 2 : 293 cell extract treated with
PMA (125ng/ml, 30min); with peptide
Predicted band size: 48 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab51164 は 1 報の論文で使用されています。
- Pang CCC et al. Investigating key factors underlying neurodegeneration linked to alpha-synuclein spread. Neuropathol Appl Neurobiol 48:e12829 (2022). PubMed: 35727707