AB32594

Anti-Synaptophysin 抗体

Anti-Synaptophysin antibody - Synaptic Marker

5

(13 Reviews)

|

(67 Publications)

Rabbit Polyclonal Synaptophysin antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 67 publications. Immunogen corresponding to Synthetic Peptide within Rat Syp aa 250-300.

別名を表示する

Synaptophysin, Major synaptic vesicle protein p38, Syp

9 Images

Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.

ab32594 is used at 1 : 1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1 : 1500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.

ab32594 is used at 1 : 1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1 : 1500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.

ab32594 is used at 1 : 1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1 : 1500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.

ab32594 is used at 1 : 1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1 : 1500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.

ab32594 is used at 1 : 1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1 : 1500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.

ab32594 is used at 1 : 1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1 : 1500 dilution.

Western blot - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

WB

Supplier Data

Western blot - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

Western blot analysis was performed on tissue extracts (30 μg lysate). The blot was probed with ab32594 (1 : 1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate. A 34 kDa band corresponding to Synaptophysin was observed in Mouse Brain, Rat Brain and not observed in other tissues which are documented to be Synaptophysin negative.

All lanes:

Western blot - Anti-Synaptophysin antibody - Synaptic Marker (ab32594) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 30 µg

Lane 2:

Rat brain tissue lysate at 30 µg

Lane 3:

Rat liver tissue lysate at 30 µg

Lane 4:

Rat kidney tissue lysate at 30 µg

Predicted band size: 33 kDa

false

Western blot - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

WB

Unknown

Western blot - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

All lanes:

Western blot - Anti-Synaptophysin antibody - Synaptic Marker (ab32594) at 1/5000 dilution

All lanes:

Rat brain

Predicted band size: 33 kDa

Observed band size: 33.8 kDa

false

Western blot - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

WB

AbReview33237****

Western blot - Anti-Synaptophysin antibody - Synaptic Marker (AB32594)

All lanes:

Western blot - Anti-Synaptophysin antibody - Synaptic Marker (ab32594) at 1/1000 dilution

All lanes:

Apteronotus leptorhynchus brain tissue lysate at 50 µg

Secondary

All lanes:

AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG at 1/1000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa,35 kDa

true

Exposure time: 7min

This image is courtesy of an anonymous Abreview

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Rat

アプリケーション

ICC/IF, WB

免疫原

Synthetic Peptide within Rat Syp aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.

Biological function summary

Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.

Pathways

Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.

Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

See full target information Syp

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文献 (67)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:111680 PubMed39868048

2025

Placode and neural crest origins of congenital deafness in mouse models of Waardenburg-Shah syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Jaime Tan,Alicia Duron,Henry M Sucov,Takako Makita

Proceedings of the National Academy of Sciences of the United States of America 121:e2400464121 PubMed39565307

2024

p.C124W variant contributes to schizophrenia by attenuating LLPS-mediated synapse formation.

Applications

Unspecified application

Species

Unspecified reactive species

Chenjun Mu,Pan Liu,Liang Liu,Yaqing Wang,Kefu Liu,Xiangyu Li,Guozhong Li,Jianbo Cheng,Mengyao Bu,Han Chen,Manpei Tang,Yuanhang Yao,Jun Guan,Tiantian Ma,Zhengrong Zhou,Qingfeng Wu,Jiada Li,Hui Guo,Kun Xia,Zhengmao Hu,Xiaoqing Peng,Bing Lang,Faxiang Li,Xiao-Wei Chen,Zhiheng Xu,Ling Yuan

Nature communications 15:8195 PubMed39294122

2024

A human Tau expressing zebrafish model of progressive supranuclear palsy identifies Brd4 as a regulator of microglial synaptic elimination.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Bai,Enhua Shao,Denglei Ma,Binxuan Jiao,Seth D Scheetz,Karen A Hartnett-Scott,Vladimir A Ilin,Elias Aizenman,Julia Kofler,Edward A Burton

Zoological research 45:535-550 PubMed38747058

2024

PCDH17 restricts dendritic spine morphogenesis by regulating ROCK2-dependent control of the actin cytoskeleton, modulating emotional behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Laidong Yu,Fangfang Zeng,Mengshu Fan,Kexuan Zhang,Jingjing Duan,Yalu Tan,Panlin Liao,Jin Wen,Chenyu Wang,Meilin Wang,Jialong Yuan,Xinxin Pang,Yan Huang,Yangzhou Zhang,Jia-Da Li,Zhuohua Zhang,Zhonghua Hu

International journal of molecular sciences 24: PubMed37373162

2023

Synaptamide Ameliorates Hippocampal Neurodegeneration and Glial Activation in Mice with Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Tyrtyshnaia,Olga Manzhulo,Igor Manzhulo

Cells 12: PubMed37296669

2023

TREM2 Agonism with a Monoclonal Antibody Attenuates Tau Pathology and Neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Fassler,Clara Benaim,Jacob George

iScience 26:106744 PubMed37207274

2023

Multi-omics analysis of a drug-induced model of bipolar disorder in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Yameng Li,Lin Zhang,Mingcai Mao,Linjuan He,Tiancai Wang,Yecan Pan,Xiaoyu Zhao,Zishu Li,Xiyan Mu,Yongzhong Qian,Jing Qiu

Neural regeneration research 18:1090-1098 PubMed36254998

2022

Neural progenitor cells derived from fibroblasts induced by small molecule compounds under hypoxia for treatment of Parkinson's disease in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Guo,Yuan-Yuan Wang,Ting-Ting Sun,Jia-Jia Xu,Pan Yang,Cai-Yun Ma,Wei-Jun Guan,Chun-Jing Wang,Gao-Feng Liu,Chang-Qing Liu

eNeuro : PubMed36216508

2022

Spontaneous Cell Cluster Formation in Human iPSC-Derived Neuronal Spheroid Networks Influences Network Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Carl-Johan Hörberg,Ulrica Englund Johansson,Fredrik Johansson,David O'Carroll

Disease models & mechanisms 15: PubMed35781563

2022

Bisphenol-A impairs synaptic formation and function by RGS4-mediated regulation of BDNF signaling in the cerebral cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Sung-Ae Hyun,Moon Yi Ko,Sumi Jang,Byoung-Seok Lee,Jaerang Rho,Kee K Kim,Woo-Yang Kim,Minhan Ka

View all publications

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