Anti-Synaptophysin 抗体 [SY38]

• IHC-P

AbReview54601****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (AB8049)

ab8049 staining Synaptophysin in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/100) for 16 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

This image is courtesy of an Abreview provided by Carl Hobbs

• IHC-P

AbReview51941****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (AB8049)

ab8049 staining MAFA in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/100 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouseIgG polyclonal (1/300) was used as the secondary antibody.

This image is courtesy of an Abreview provided by Carl Hobbs.

• Flow Cyt

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Flow Cytometry - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (AB8049)

Overlay histogram showing PC12 cells stained with ab8049 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8049, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

• WB

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Western blot - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (AB8049)

All lanes:

Western blot - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049) at 1/500 dilution

Lane 1:

Human brain tissue lysate - total protein (ab29466) at 10 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed at 1/3000 dilution

Predicted band size: 33 kDa

Observed band size: 38 kDa

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• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (AB8049)

The pictures show immunohistochemical staining of normal mucosa and neuroendocrine tumour of the small intestine. The antibody (ab8049) stains positive in a 1 : 10 dilution in nerve cells of the colon as well as some cells in normal colon mucosa and some neuroendocrine tumors of the digestive tract. Staining protocol : Mouse monoclonal to Synaptophysin (ab8049), diluted 1 : 10 in PBS plus BSA, 60 min at room temperature,
secondary antibody : HRP solution with anti mouse polyclonal antibodies, 30min. DAB staining 10 min; Hematoxylin 30 sec.

These images were kindly supplied as part of the review submitted by Karin Birkenkamp-Demtroeder.

• WB

CiteAb

Western blot - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (AB8049)

Western Blotting using Anti-Synaptophysin antibody [SY38], ab8049. Publication image from Jia, L. et al., 2020, Nat Commun, 33139712. Legend direct from paper.

APOE4 enhances apoptosis and synaptic loss in cerebral organoids from AD patients.cerebral organoids were subjected to immunostaining and western blotting at week 12. a Representative images and quantification of cellular apoptosis evaluated by immunostaining of cleaved CASP3. Scale bar : 100 µm. b Cleaved CASP3 immunoreactivities were quantified from 5 cerebral organoids per line, and the averaged values were compared among the groups (APOE4 : p = 0.032, AD : p = 0.0569, APOE4 x AD : p = 0.018, Con-E3 vs. AD-E4 : p = 0.0523, Con-E4 vs. AD-E4 : p = 0.0112, AD-E3 vs. AD-E4 : p = 0.014). All data are expressed as mean ± SEM (N = 5). c–f Cleaved CASP3, CASP3, synaptophysin, PSD95, and Tuj1 levels in the lysates of 4–5 cerebral organoids per line were analyzed by western blotting and quantified. All data are expressed as mean ± SEM (N = 5). d Cleaved CASP3 levels were normalized to total CASP3 levels and compared among groups (APOE4 : p < 0.0001, AD : p < 0.0001, APOE4 x AD : p = 0.0020, Con-E3 vs. Con-E4 : p = 0.009, Con-E3 vs. AD-E3 : p = 0.0206, Con-E4 vs. AD-E4 : p < 0.0001, AD-E3 vs. AD-E4 : p < 0.0001). Synaptophysin and PSD95 levels were normalized to Tuj1 levels and compared among groups (eAPOE4 : p = 0.5841, AD : p = 0.0002, APOE4 x AD : p = 0.0453, Con-E3 vs. AD-E4 : p = 0.0069, Con-E4 vs. AD-E4 : p = 0.0002, Con-E4 vs. AD-E3 : p = 0.0077. fAPOE4 : p = 0.8794, AD : p = 0.0025, APOE4 x AD : p = 0.0551, Con-E4 vs. AD-E3 : p = 0.0404, Con-E4 vs. AD-E4 : 0.0019). ANCOVA for APOE4, AD status, and APOE4 x AD status was performed by including sex, sampling age, and source of iPSCs as co-variables, which was followed by two-sided Tukey–Kramer tests to compare between the groups with two factors (APOE4 and AD status). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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• WB

CiteAb

Western blot - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (AB8049)

Western Blotting using Anti-Synaptophysin antibody [SY38], ab8049. Publication image from Jackson, G. R. et al., 2011, Mol Neurodegener, 21645391. Legend direct from paper.

Tau oligomers induce synaptic dysfunction. (A) Representative Western blot of mouse hippocampus homogenate. The levels of synaptophysin, synapsin-1, and septin-11 were measured by band quantification and normalized with the levels of tubulin. PBSo indicates representative bands of hippocampal area injected with PBS in mice also injected with tau oligomers, PBSf indicates PBS injection in mice also injected with tau fibrils, and PBSm indicates PBS injection in mice also injected with tau monomers. (B) Synaptophysin levels were significantly lower in the hemispheres injected with tau oligomers in comparison with the ones injected with fibrils, monomers, or PBS. (C) No significant differences in the levels of synapsin-1 were observed. (D) Only the hemisphere injected with tau oligomers presents a decrease in the level of septin-11. Data are represented as the mean ± SE. *p < 0.01, n = 6

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