Anti-Synaptophysin 抗体 (ab32594)
Key features and details
- Rabbit polyclonal to Synaptophysin
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Synaptophysin antibody
Synaptophysin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Synaptophysin -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Rat
交差が予測される動物種: Human
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免疫原
Synthetic peptide:
GPGGYGPQDSYGPQGGYQPD
, corresponding to amino acids 253-272 of Rat Synaptophysin -
ポジティブ･コントロール
- WB: Mouse and rat brain tissue lysates. ICC/IF: rat hippocampal neurons
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32594の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (5) |
1/5000. Detects a band of approximately 33.8 kDa (predicted molecular weight: 34 kDa).
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
1/5000. Detects a band of approximately 33.8 kDa (predicted molecular weight: 34 kDa). |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. -
組織特異性
Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. -
関連疾患
Mental retardation, X-linked, SYP-related -
配列類似性
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain. -
ドメイン
The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein. -
翻訳後修飾
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
細胞内局在
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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参照データベース
- Entrez Gene: 6855 Human
- Entrez Gene: 24804 Rat
- Omim: 313475 Human
- SwissProt: P08247 Human
- SwissProt: P07825 Rat
- Unigene: 632804 Human
- Unigene: 11067 Rat
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別名
- Major synaptic vesicle protein p38 antibody
- MRX96 antibody
- MRXSYP antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
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Western blot - Anti-Synaptophysin antibody (ab32594)All lanes : Anti-Synaptophysin antibody (ab32594) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : Rat kidney tissue lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDaWestern blot analysis was performed on tissue extracts (30 µg lysate). The blot was probed with ab32594 (1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate. A 34 kDa band corresponding to Synaptophysin was observed in Mouse Brain, Rat Brain and not observed in other tissues which are documented to be Synaptophysin negative.
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Western blot - Anti-Synaptophysin antibody (ab32594)This image is courtesy of an anonymous AbreviewAnti-Synaptophysin antibody (ab32594) at 1/1000 dilution + Apteronotus leptorhynchus brain tissue lysate at 50 µg
Secondary
AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG
at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 33,35 kDa why is the actual band size different from the predicted?
Exposure time: 7 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
-
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
-
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
-
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
-
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
-
Western blot - Anti-Synaptophysin antibody (ab32594)Anti-Synaptophysin antibody (ab32594) at 1/5000 dilution + Rat brain
Predicted band size: 34 kDa
Observed band size: 33.8 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (60)
ab32594 は 60 報の論文で使用されています。
- Guo Y et al. Neural progenitor cells derived from fibroblasts induced by small molecule compounds under hypoxia for treatment of Parkinson's disease in rats. Neural Regen Res 18:1090-1098 (2023). PubMed: 36254998
- Li Y et al. Multi-omics analysis of a drug-induced model of bipolar disorder in zebrafish. iScience 26:106744 (2023). PubMed: 37207274
- Tyrtyshnaia A et al. Synaptamide Ameliorates Hippocampal Neurodegeneration and Glial Activation in Mice with Traumatic Brain Injury. Int J Mol Sci 24:N/A (2023). PubMed: 37373162
- Reifschneider A et al. Loss of TREM2 rescues hyperactivation of microglia, but not lysosomal deficits and neurotoxicity in models of progranulin deficiency. EMBO J 41:e109108 (2022). PubMed: 35019161
- Kútna V et al. Cerebellar demyelination and neurodegeneration associated with mTORC1 hyperactivity may contribute to the developmental onset of autism-like neurobehavioral phenotype in a rat model. Autism Res 15:791-805 (2022). PubMed: 35178882