Anti-Synaptophysin 抗体 (ab14692)
Key features and details
- Rabbit polyclonal to Synaptophysin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Synaptophysin antibody
Synaptophysin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Synaptophysin -
由来種
Rabbit -
特異性
The antibody does not cross-react other synaptic vesicle proteins such as synaptotagmin, GAP45, and SNAP25 protiens. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Synaptophysin aa 41-62.
Sequence:FATCGSYSGELQLSVDCANKTE
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ポジティブ・コントロール
- IHC: Rat and mouse brain. WB: Mouse forebrain extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
The buffer includes stabilizing proteins and glycerol. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14692の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/200 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
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IHC-P | (2) |
1/100.
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特記事項 |
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WB
1/200 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa). |
IHC-P
1/100. |
ターゲット情報
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機能
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. -
組織特異性
Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. -
関連疾患
Mental retardation, X-linked, SYP-related -
配列類似性
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain. -
ドメイン
The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein. -
翻訳後修飾
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
細胞内局在
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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参照データベース
- Entrez Gene: 20977 Mouse
- Entrez Gene: 24804 Rat
- SwissProt: Q62277 Mouse
- SwissProt: P07825 Rat
- Unigene: 223674 Mouse
- Unigene: 11067 Rat
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別名
- Major synaptic vesicle protein p38 antibody
- MRX96 antibody
- MRXSYP antibody
see all
画像
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Immunohistochemical analysis of formaldehyde-fixed, paraffin-embedded mouse brain sections labelling Synaptophysin with ab14692 at a dilution of 1/100 In IHC blocking Buffer. DAB (brown_ staining and Hematoxylin QS (blue) counterstain was used. 20x magnfication of Leica DM4000.
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Synaptophysin antibody (ab14692) western blot on mouse forebrain extract (10µg protein/lane). The blot was exposed for 10 minutes to autoradiographic film. Ladder markers from 7-194 kDa.
Lane 1 = 1/500 ab14692
Lane 2 = 1/200 ab14692 -
Immunohistochemical analysis of formaldehyde-fixed, paraffin-embedded rat brain sections labelling Synaptophysin with ab14692 at a dilution of 1/100 In IHC blocking Buffer. DAB (brown_ staining and Hematoxylin QS (blue) counterstain was used. 20x magnfication of Leica DM4000.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (117)
ab14692 は 117 報の論文で使用されています。
- Yoon KN et al. Tomato and lemon extracts synergistically improve cognitive function by increasing brain-derived neurotrophic factor levels in aged mice. Br J Nutr 131:1105-1114 (2024). PubMed: 38016800
- Rodriguez-Ortiz CJ et al. Angiotensin receptor blockade with olmesartan alleviates brain pathology in obese OLETF rats. Clin Exp Pharmacol Physiol 50:228-237 (2023). PubMed: 36398458
- Shi D et al. The Anaesthetics Isoflurane and Xenon Reverse the Synaptotoxic Effects of Aβ1-42 on Megf10-Dependent Astrocytic Synapse Elimination and Spine Density in Ex Vivo Hippocampal Brain Slices. Int J Mol Sci 24:N/A (2023). PubMed: 36674434
- Bathini P et al. Systemic Inflammation Causes Microglial Dysfunction With a Vascular AD phenotype. Brain Behav Immun Health 28:100568 (2023). PubMed: 36704658
- Kontaxi C et al. Epilepsy-Related CDKL5 Deficiency Slows Synaptic Vesicle Endocytosis in Central Nerve Terminals. J Neurosci 43:2002-2020 (2023). PubMed: 36759195