Anti-Synaptojanin 抗体 [AC1] (ab24591)
![Immunocytochemistry/ Immunofluorescence - Anti-Synaptojanin antibody [AC1] (ab24591)](https://www.abcam.co.jp/ps/products/24/ab24591/Images/ab24591-35370-anti-synaptojanin-antibody-ac1-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Synaptojanin antibody [AC1] (ab24591)")
Key features and details
- Mouse monoclonal [AC1] to Synaptojanin
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: unknown
製品の概要
-
製品名
Anti-Synaptojanin antibody [AC1]
Synaptojanin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [AC1] to Synaptojanin -
由来種
Mouse -
特異性
ab24591 is specific for rat synsptojanin 1 protein. It may cross-react only very weakly with human synaptojanin 1. ab24591 does not cross react with mouse. -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Rat -
免疫原
Recombinant fragment, corresponding to C terminal amino acids 1156-1286 of Rat Synaptojanin
-
特記事項
The C-terminal proline-rich region of Synaptojanin 1 mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
The C-terminal proline-rich region of Synaptojanin 1 mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2. -
ポリ/モノ
モノクローナル -
クローン名
AC1 -
アイソタイプ
unknown -
研究分野
関連製品
-
Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24591の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
1/20 - 1/100.
|
| 特記事項 |
|---|
|
ICC/IF
1/20 - 1/100. |
ターゲット情報
-
機能
Inositol 5-phosphatase which has a role in clathrin-mediated endocytosis. -
組織特異性
Concentrated at clathrin-coated endocytic intermediates in nerve terminals. Isoform 1 is more enriched than isoform 2 in developing brain as well as non-neuronal cells. Isoform 2 is very abundant in nerve terminals. -
配列類似性
Belongs to the synaptojanin family.
In the central section; belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SAC domain. -
ドメイン
Binds to EPS15 (a clathrin coat-associated protein) via a C-terminal domain containing three Asn-Pro-Phe (NPF) repeats.
The C-terminal proline-rich region mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 85238 Rat
- SwissProt: Q62910 Rat
- Unigene: 22685 Rat
-
別名
- 4 antibody
- 5-trisphosphate 5-phosphatase 1 antibody
- Inositol 5' phosphatase (synaptojanin 1) antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-Synaptojanin antibody [AC1] (ab24591)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France
Immunofluorescence staining using Mouse monoclonal [AC1] to Synaptojanin - Synaptic Marker (ab24591; 30 µg/ml ) on rat Hippocampal neuron culture (paraformadlehyde fixed - see review). Confocal image showing blue nuclear counterstain.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (5)
ab24591 は 5 報の論文で使用されています。
- Latimer CS et al. Vitamin D prevents cognitive decline and enhances hippocampal synaptic function in aging rats. Proc Natl Acad Sci U S A 111:E4359-66 (2014). Rat . PubMed: 25267625
- Nemoto Y et al. Identification and characterization of a synaptojanin 2 splice isoform predominantly expressed in nerve terminals. J Biol Chem 276:41133-42 (2001). PubMed: 11498538
- Haffner C et al. Synaptojanin 1: localization on coated endocytic intermediates in nerve terminals and interaction of its 170 kDa isoform with Eps15. FEBS Lett 419:175-80 (1997). PubMed: 9428629
- Ringstad N et al. The SH3p4/Sh3p8/SH3p13 protein family: binding partners for synaptojanin and dynamin via a Grb2-like Src homology 3 domain. Proc Natl Acad Sci U S A 94:8569-74 (1997). PubMed: 9238017
- Nemoto Y et al. Synaptojanin 2, a novel synaptojanin isoform with a distinct targeting domain and expression pattern. J Biol Chem 272:30817-21 (1997). PubMed: 9388224