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Anti-SYBL1/VAMP-7 抗体 [158.2] (ab36195)

Reviews (1)Q&A (1)References (26)
Western blot - Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195)
  • Flow Cytometry - Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195)

Key features and details

  • Mouse monoclonal [158.2] to SYBL1/VAMP-7
  • Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WB
  • Reacts with: Rat, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-SYBL1/VAMP-7 antibody [158.2]
    SYBL1/VAMP-7 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [158.2] to SYBL1/VAMP-7

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WBmore details

  • 種交差性

    交差種: Rat, Human
    交差が予測される動物種: Mouse, Dog

  • 免疫原

    Recombinant full length protein (6x HIS-tag) corresponding to Human SYBL1/VAMP-7 aa 100-200. Recombinant domain of TI-VAMP encompassing aa 119–188 fused to 6xhis emulsified in complete Freund's adjuvant.
    Database link: P51809

  • ポジティブ･コントロール

    • extract from rat brain

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab36195の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF (1)
Use at an assay dependent concentration. PubMed: 19138172
Flow Cyt
1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
1/500 - 1/2000. Predicted molecular weight: 24 kDa.
特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 19138172
Flow Cyt
1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
1/500 - 1/2000. Predicted molecular weight: 24 kDa.

ターゲット情報

  • 機能

    Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.

  • 組織特異性

    Detected in all tissues tested.

  • 配列類似性

    Belongs to the synaptobrevin family.
    Contains 1 longin domain.
    Contains 1 v-SNARE coiled-coil homology domain.

  • 細胞内局在

    Cytoplasmic vesicle > secretory vesicle membrane. Golgi apparatus > trans-Golgi network membrane. Late endosome membrane. Lysosome membrane. Endoplasmic reticulum membrane. Cytoplasmic vesicle > phagosome membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • FLJ53045 antibody
    • FLJ53762 antibody
    • FLJ54296 antibody
    • HGNC:11486 antibody
    • OTTHUMP00000024258 antibody
    • OTTHUMP00000024259 antibody
    • OTTHUMP00000225953 antibody
    • SYBL 1 antibody
    • SYBL1 antibody
    • Synaptobrevin like 1 antibody
    • Synaptobrevin-like protein 1 antibody
    • Tetanus insensitive VAMP antibody
    • Tetanus neurotoxin insensitive VAMP antibody
    • Tetanus-insensitive VAMP antibody
    • TI VAMP antibody
    • Ti-VAMP antibody
    • TIVAMP antibody
    • VAMP-7 antibody
    • VAMP7 antibody
    • VAMP7_HUMAN antibody
    • Vesicle-associated membrane protein 7 antibody
    see all

画像

  • Western blot - Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195)
    Western blot - Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195)
    Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195) at 1/500 dilution + Tissue lysates prepared from rat brain

    Predicted band size: 24 kDa

  • Flow Cytometry - Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195)
    Flow Cytometry - Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195)
    Overlay histogram showing PC12 cells stained with ab36195 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36195, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

参考文献 (26)

ab36195 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab36195 は 26 報の論文で使用されています。

  • Vijayan K  et al. A genome-wide CRISPR-Cas9 screen identifies CENPJ as a host regulator of altered microtubule organization during Plasmodium liver infection. Cell Chem Biol 29:1419-1433.e5 (2022). PubMed: 35738280
  • West ZE  et al. The trans-SNARE complex VAMP4/Stx6/Stx7/Vti1b is a key regulator of Golgi to late endosome MT1-MMP transport in macrophages. Traffic 22:368-376 (2021). PubMed: 34476885
  • Hülsemann M  et al. TC10 regulates breast cancer invasion and metastasis by controlling membrane type-1 matrix metalloproteinase at invadopodia. Commun Biol 4:1091 (2021). PubMed: 34531530
  • Tian X  et al. DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B. Autophagy 16:797-810 (2020). PubMed: 31251111
  • Liu Y  et al. Ablation of All Synaptobrevin vSNAREs Blocks Evoked But Not Spontaneous Neurotransmitter Release at Neuromuscular Synapses. J Neurosci 39:6049-6066 (2019). PubMed: 31160536
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-SYBL1/VAMP-7 antibody [158.2]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Chinese hamster Cell (Ovary (CHO-K1 - tissue culture))
Permeabilization
Yes - TX-100
Specification
Ovary (CHO-K1 - tissue culture)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Diana Thomas

Verified customer

投稿 Jun 03 2010

Question

Customer kindly called as this product did not work in Western blot on human tissues.

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Answer

Thank you for contacting us. Your credit note ID is XXXX. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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