Anti-SV40 VP2 + VP3 抗体 (ab53983)
Key features and details
- Rabbit polyclonal to SV40 VP2 + VP3
- Suitable for: WB
- Reacts with: Simian Virus 40
- Isotype: IgG
製品の概要
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製品名
Anti-SV40 VP2 + VP3 antibody -
製品の詳細
Rabbit polyclonal to SV40 VP2 + VP3 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Simian Virus 40 -
免疫原
Recombinant fragment corresponding to SV40 VP2 + VP3.
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特記事項
This antibody recognises two of the SV40 capsid proteins - VP2 and VP3
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: Whole serum, 50% Glycerol -
Concentration information loading... -
精製度
Whole antiserum -
一次抗体 備考
This antibody recognises two of the SV40 capsid proteins - VP2 and VP3 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53983の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 38,26 kDa.
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| 特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 38,26 kDa. |
ターゲット情報
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関連性
There are three simian virus 40 (SV40) capsid proteins - VP1, VP2 and VP3. VP1 assembles into pentamers which have a central cavity that contains a copy of one of the other capsid proteins, VP2 or VP3. This complex is then imported into the cell nucleus. Here, 72 of the complexes assemble around the newly synthesised viral genome to form the icosahedral capsid. -
参照データベース
- SwissProt: P03093 Simian Virus 40
画像
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Western blot - Anti-SV40 VP2 + VP3 antibody (ab53983)This image is courtesy of an anonymous AbreviewAll lanes : Anti-SV40 VP2 + VP3 antibody (ab53983) at 1/5000 dilution
Lane 1 : BK virus infected 293T cell lysate
Lane 2 : Mock 293T cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38,26 kDa
Observed band size: 27,38 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutes
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Western blot - Anti-SV40 VP2 + VP3 antibody (ab53983)SV40 was ultracentrifuged on CsCl-gradient, fractions were collected from the top of the tube, run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fractions 3,4 and 6 were run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fraction 3,4 and 6 were run on PAGe and analysed by Western blot with α-VP1 and α-VP2/3
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (20)
ab53983 は 20 報の論文で使用されています。
- Spriggs CC et al. Components of the LINC and NPC complexes coordinately target and translocate a virus into the nucleus to promote infection. PLoS Pathog 18:e1010824 (2022). PubMed: 36067270
- Bagchi P et al. Lunapark-dependent formation of a virus-induced ER exit site contains multi-tubular ER junctions that promote viral ER-to-cytosol escape. Cell Rep 37:110077 (2021). PubMed: 34879280
- Spriggs CC et al. Golgi-associated BICD adaptors couple ER membrane penetration and disassembly of a viral cargo. J Cell Biol 219:N/A (2020). PubMed: 32259203
- Panou MM et al. Glibenclamide inhibits BK polyomavirus infection in kidney cells through CFTR blockade. Antiviral Res 178:104778 (2020). PubMed: 32229236
- Chen YJ et al. Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes. J Cell Biol 219:N/A (2020). PubMed: 31895406