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AB324578

Anti-SV2A 抗体 [EPR23500-32] - BSA and Azide free (Capture)

Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal SV2A antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse samples.

別名を表示する

Kiaa0736, Sv2, Sv2a, Synaptic vesicle glycoprotein 2A, Synaptic vesicle protein 2, Synaptic vesicle protein 2A, Calcium regulator SV2A

2 Images
Sandwich ELISA - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (Capture) (AB324578)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (Capture) (AB324578)

Interpolated concentration of native SV2A was measured at different sample concentrations. Undiluted samples are as follows : human brain tissue extract (50 µg/mL), mouse brain tissue extract (50 µg/mL) and SH-SY5Y cell extract (1000 µg/mL). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (Capture) (AB324578)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (Capture) (AB324578)

Sandwich ELISA using Human SV2A protein standard curve with the capture antibody (ab324578) at 2 µg/mL and detector antibody (ab324579) at 0.5 µg/mL. Background-subtracted absorbance data values (mean +/- standard deviation are shown.)

関連する標識済み抗体及び組成の異なる製品 (4)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR23500-32

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human, Mouse

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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製品の詳細

ab324578 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse SV2A. The recommended pair for sandwich ELISA is:

Capture: ab324578, Human/Mouse SV2A Capture Antibody (unconjugated)

Detector: ab324579, Human/Mouse SV2A Detector Antibody (unconjugated)

The reference range value is 19.5-1250 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles.. (Microbial infection) Receptor for C.botulinum neurotoxin type A (BoNT/A, botA); the toxin probably binds via extracellular loop 4 (PubMed : 16543415).. (Microbial infection) Possible receptor for C.botulinum neurotoxin type D (BoNT/D, botD); BoNT/D does not bind to extracellular loop 4 as do BoNT/A and BoNT/E (PubMed : 21483489).. (Microbial infection) Receptor for C.botulinum neurotoxin type E (BoNT/E); the toxin probably binds via extracellular loop 4 (PubMed : 18815274). It probably requires glycosylation of Asn-573 (PubMed : 18815274).
See full target information Sv2a
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Abcam product promise

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