Anti-SV2A 抗体 (ab77177)
Key features and details
- Goat polyclonal to SV2A
- Suitable for: WB, ICC
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SV2A antibody
SV2A 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to SV2A -
由来種
Goat -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Horse, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey
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免疫原
Synthetic peptide:
C-TVELYPSDKRTTA
, corresponding to internal sequence amino acids 673-685 of Human SV2A (NP_055664.2) -
ポジティブ･コントロール
- WB: Rat brain tissue lysate. ICC: KNRK and U-2 OS cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab77177 was purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77177の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa).
A 1 hour primary incubation is recommended for this product. |
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| ICC |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa). A 1 hour primary incubation is recommended for this product. |
|
ICC
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles. -
配列類似性
Belongs to the major facilitator superfamily. -
翻訳後修飾
Phosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1.
N-glycosylated. -
細胞内局在
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Enriched in chromaffin granules, not present in adrenal microsomes. Associated with both insulin granules and synaptic-like microvesicles in insulin-secreting cells of the pancreas. - Information by UniProt
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参照データベース
- Entrez Gene: 280936 Cow
- Entrez Gene: 9900 Human
- Entrez Gene: 64051 Mouse
- Entrez Gene: 117559 Rat
- Omim: 185860 Human
- SwissProt: Q29397 Cow
- SwissProt: Q7L0J3 Human
- SwissProt: Q9JIS5 Mouse
see all -
別名
- KIAA0736 antibody
- OTTHUMP00000014065 antibody
- SV2 antibody
see all
画像
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Immunocytochemistry - Anti-SV2A antibody (ab77177)
Immunofluorescence analysis of KNRK (rat normal kidney cell line) cells labelling SV2A with ab77177 at 10 µg/mL.
Cells were permeabilized with 0.15% Triton X-100. Alexa Fluor 488® secondary antibody (2 µg/mL) was used as the secondary antibody (green). Nuclear DNA was labeled with DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488® secondary antibody (2 µg/mL).
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Western blot - Anti-SV2A antibody (ab77177)All lanes : Anti-SV2A antibody (ab77177) at 0.3 µg/ml
Lane 1 : Rat brain lysate in RIPA buffer
Lane 2 : Rat brain lysate in RIPA buffer treated with immunising peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 83 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Primary antibody incubation: 1 hour at room temperature.
An additonal band of unknown identity was also consistently observed at 28kDa, which was successfully blocked by incubation with the immunising peptide.
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Immunocytochemistry - Anti-SV2A antibody (ab77177)Immunofluorescence analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells labelling SV2A with ab77177 at 10 µg/mL.
Cells were permeabilized with 0.15% Triton X-100. Alexa Fluor 488® secondary antibody (2 µg/mL) was used as the secondary antibody (green). Nuclear DNA was labeled with DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488® secondary antibody (2 µg/mL).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab77177 は論文での使用が確認できていません。