Anti-SV2A 抗体 (ab32942)
Key features and details
- Rabbit polyclonal to SV2A
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SV2A antibody
SV2A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SV2A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Cow, Human
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: mouse and rat brain.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32942の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 82-92 kDa (predicted molecular weight: 83 kDa).
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| IP | (1) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 82-92 kDa (predicted molecular weight: 83 kDa). |
|
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles. -
配列類似性
Belongs to the major facilitator superfamily. -
翻訳後修飾
Phosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1.
N-glycosylated. -
細胞内局在
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Enriched in chromaffin granules, not present in adrenal microsomes. Associated with both insulin granules and synaptic-like microvesicles in insulin-secreting cells of the pancreas. - Information by UniProt
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参照データベース
- Entrez Gene: 280936 Cow
- Entrez Gene: 9900 Human
- Entrez Gene: 64051 Mouse
- Entrez Gene: 117559 Rat
- Omim: 185860 Human
- SwissProt: Q29397 Cow
- SwissProt: Q7L0J3 Human
- SwissProt: Q9JIS5 Mouse
see all -
別名
- KIAA0736 antibody
- OTTHUMP00000014065 antibody
- SV2 antibody
see all
画像
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Western blot - Anti-SV2A antibody (ab32942)All lanes : Anti-SV2A antibody (ab32942) at 1 µg/ml
Lane 1 : Mouse brain
Lane 2 : Rat brain
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 83 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking buffer: 2% BSA.
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Immunoprecipitation - Anti-SV2A antibody (ab32942)SV2A was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to SV2A and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32942.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 82kDa: SV2A
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (37)
ab32942 は 37 報の論文で使用されています。
- Kontaxi C et al. Epilepsy-Related CDKL5 Deficiency Slows Synaptic Vesicle Endocytosis in Central Nerve Terminals. J Neurosci 43:2002-2020 (2023). PubMed: 36759195
- Zhu Z et al. Acrolein, an endogenous aldehyde induces synaptic dysfunction in vitro and in vivo: Involvement of RhoA/ROCK2 pathway. Aging Cell 21:e13587 (2022). PubMed: 35315217
- Fukuyama K & Okada M Brivaracetam and Levetiracetam Suppress Astroglial L-Glutamate Release through Hemichannel via Inhibition of Synaptic Vesicle Protein. Int J Mol Sci 23:N/A (2022). PubMed: 35562864
- Dhandapani R et al. Sustained Trem2 stabilization accelerates microglia heterogeneity and Aβ pathology in a mouse model of Alzheimer's disease. Cell Rep 39:110883 (2022). PubMed: 35649351
- Nehme R et al. The 22q11.2 region regulates presynaptic gene-products linked to schizophrenia. Nat Commun 13:3690 (2022). PubMed: 35760976