Anti-SUZ12 抗体 (ab12073)
Key features and details
- Rabbit polyclonal to SUZ12
- Suitable for: IP, ChIP, ChIP/Chip, IHC-P, WB, RIP
- Knockout validated
- Reacts with: Mouse, Human, Common marmoset
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SUZ12 antibody
SUZ12 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SUZ12 -
由来種
Rabbit -
アプリケーション
適用あり: IP, ChIP, ChIP/Chip, IHC-P, WB, RIPmore details -
種交差性
交差種: Mouse, Human, Common marmoset -
免疫原
Synthetic peptide corresponding to Human SUZ12 aa 700 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab12389) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12073の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | (3) |
Use at an assay dependent concentration.
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| ChIP | (9) |
Use at an assay dependent concentration.
|
| ChIP/Chip | (1) |
Use at an assay dependent concentration.
|
| IHC-P | (2) |
Use at an assay dependent concentration.
|
| WB | (9) |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 83 kDa).
|
| RIP | (2) |
Use at an assay dependent concentration. PubMed: 19571010
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| 特記事項 |
|---|
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IP
Use at an assay dependent concentration. |
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ChIP
Use at an assay dependent concentration. |
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ChIP/Chip
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 83 kDa). |
|
RIP
Use at an assay dependent concentration. PubMed: 19571010 |
ターゲット情報
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機能
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. -
組織特異性
Overexpressed in breast and colon cancer. -
関連疾患
Note=A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer. -
配列類似性
Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.
Contains 1 C2H2-type zinc finger. -
発生段階
Expressed at low levels in quiescent cells. Expression rises at the G1/S phase transition. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 23512 Human
- Entrez Gene: 52615 Mouse
- Omim: 606245 Human
- SwissProt: Q15022 Human
- SwissProt: Q80U70 Mouse
- Unigene: 462732 Human
- Unigene: 283410 Mouse
- Unigene: 473315 Mouse
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別名
- ChET 9 protein antibody
- CHET9 antibody
- Chromatin precipitated E2F target 9 protein antibody
see all
画像
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Western blot - Anti-SUZ12 antibody (ab12073)All lanes : Anti-SUZ12 antibody (ab12073) at 1 µg/ml
Lane 1 : Wild-type Hela cell lysate
Lane 2 : SUZ12 knockout HeLa cell lysate
Lane 3 : Wild-type HAP1 cell lysate
Lane 4 : SUZ12 knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab12073 was shown to react with SUZ12 in western blot. The band observed in the SUZ12 knockout cell line ab264983 (SUZ12 knockout cell lysate ab257721) lane below 95 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab12073 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Western blot - Anti-SUZ12 antibody (ab12073)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SUZ12 knockout HAP1 cell lysate (20 µg)
Lane 3: Caco2 cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - loading control, ab8245, observed at 37 kDa.ab12073 was shown to specifically recognize SUZ12 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when SUZ12 knockout samples were used. Wild-type and SUZ12 knockout samples were subjected to SDS-PAGE. ab12073 and ab8245 (loading control to GAPDH) were diluted to 1µg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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Western blot - Anti-SUZ12 antibody (ab12073)All lanes : Anti-SUZ12 antibody (ab12073)
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SUZ12 CRISPR/Cas9 edited HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Lanes 1- 2: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab12073 was shown to react with SUZ12 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab264983 (CRISPR/Cas9 edited cell lysate ab257721) lane below 95kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and SUZ12 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab12073 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 µg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-SUZ12 antibody - ChIP Grade (ab12073)All lanes : Anti-SUZ12 antibody (ab12073) at 1 µg/ml
Lane 1 : untransfected 293T cell lysate
Lane 2 : 293T cells transfected with 5ug HA-Suz12
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG H&L (HRP) (Dako) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 76 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-SUZ12 antibody - ChIP Grade (ab12073)All lanes : Anti-SUZ12 antibody (ab12073) at 1 µg/ml
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa, 53 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (147)
ab12073 は 147 報の論文で使用されています。
- Yu X et al. Dissecting and targeting noncanonical functions of EZH2 in multiple myeloma via an EZH2 degrader. Oncogene 42:994-1009 (2023). PubMed: 36747009
- Schaefer EJ et al. BCOR and BCORL1 Mutations Drive Epigenetic Reprogramming and Oncogenic Signaling by Unlinking PRC1.1 from Target Genes. Blood Cancer Discov 3:116-135 (2022). PubMed: 35015684
- Alvarez-Dominguez JR et al. An adipose lncRAP2-Igf2bp2 complex enhances adipogenesis and energy expenditure by stabilizing target mRNAs. iScience 25:103680 (2022). PubMed: 35036870
- Bowness JS et al. Xist-mediated silencing requires additive functions of SPEN and Polycomb together with differentiation-dependent recruitment of SmcHD1. Cell Rep 39:110830 (2022). PubMed: 35584662
- Huang X et al. A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency. Cell Rep 39:110928 (2022). PubMed: 35675764