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リコンビナントRabMAb

Anti-Survivin 抗体 [EPR2675] - BSA and Azide free (ab167450)

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Western blot - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
  • Western blot - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
  • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
  • Flow Cytometry - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
  • Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2675] to Survivin - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC HRP PE Unconjugated

製品の概要

  • 製品名

    Anti-Survivin antibody [EPR2675] - BSA and Azide free
    Survivin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2675] to Survivin - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Flow Cyt, ICC/IF, IHC-Pmore details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: NIH/3T3 and HeLa cells; IHC-P: Human tonsil, Mouse and Rat spleen tissue; Flow Cyt: MCF7 cells. WB: HeLa, NIH/3T3, C6, Ramos and F9 whole cell lysates.

  • 特記事項

    ab167450 is the carrier-free version of ab134170.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab167450の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

  • 組織特異性

    Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.

  • 配列類似性

    Belongs to the IAP family.
    Contains 1 BIR repeat.

  • 発生段階

    Expression is cell cycle-dependent and peaks at mitosis.

  • ドメイン

    The BIR repeat is necessary and sufficient for HBXIP binding.

  • 翻訳後修飾

    Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.

  • 細胞内局在

    Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • API4 antibody
    • Apoptosis inhibitor 4 antibody
    • Apoptosis inhibitor survivin antibody
    • Apoptosis inhibitor4 antibody
    • Baculoviral IAP repeat containing 5 antibody
    • Baculoviral IAP repeat containing protein 5 antibody
    • Baculoviral IAP repeat-containing protein 5 antibody
    • BIRC 5 antibody
    • BIRC5 antibody
    • BIRC5_HUMAN antibody
    • EPR 1 antibody
    • IAP4 antibody
    • Survivin variant 3 alpha antibody
    • SVV antibody
    • TIAP antibody
    see all

画像

  • Western blot - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    Western blot - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    All lanes : Anti-Survivin antibody [EPR2675] (ab134170) at 1/1000 dilution (Purified)

    Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100ng/ml nocodazole for 24 hours whole cell lysate
    Lane 3 : Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100ng/ml nocodazole for 24 hours whole cell lysate
    Lane 5 : Untreated C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 6 : C6 (Rat glial tumor glial cell) treated with 100ng/ml nocodazole for 24 hours whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 16 kDa

  • Western blot - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    Western blot - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    All lanes : Anti-Survivin antibody [EPR2675] (ab134170) at 1/1000 dilution (Purified)

    Lane 1 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 2 : F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 16 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

    This data was developed using ab134170, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling Survivin with purified ab134170 at 1/800 dilution (2.67 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

    This data was developed using ab134170, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Bad with purified ab32445 at 1/50 dilution (2.9 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    Flow Cytometry - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    This data was developed using ab134170, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Survivin with purified ab134170 at 1/200 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

    This data was developed using ab134170, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling Survivin with purified ab134170 at 1/800 dilution (2.67 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

    This data was developed using ab134170, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling Survivin with purified ab134170 at 1/800 dilution (2.67 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab167450 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab167450 は論文での使用が確認できていません。

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