Anti-Surfactant Protein A/PSAP 抗体 (ab115791)
Key features and details
- Rabbit polyclonal to Surfactant Protein A/PSAP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Surfactant Protein A/PSAP antibody -
製品の詳細
Rabbit polyclonal to Surfactant Protein A/PSAP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Synthetic peptide within Mouse Surfactant Protein A/PSAP aa 200 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. The region of Mouse Surfactant Protein A/PSAP used for the immunogen is identical to the related Rat sequence.
Database link: P35242 -
ポジティブ･コントロール
- WB: Mouse and rat lung tissue lysate; IHC-P: Mouse and rat lung tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115791の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 26 kDa.
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| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 26 kDa. |
|
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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細胞内局在
Secreted protein; extracellular space. -
参照データベース
- Entrez Gene: 20387 Mouse
- Entrez Gene: 24773 Rat
- SwissProt: P35242 Mouse
- SwissProt: P08427 Rat
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別名
- 35 kDa pulmonary surfactant-associated protein antibody
- Alveolar proteinosis protein antibody
- COLEC4 antibody
see all
画像
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Western blot - Anti-Surfactant Protein A/PSAP antibody (ab115791)All lanes : Anti-Surfactant Protein A/PSAP antibody (ab115791) at 0.5 µg/ml
Lane 1 : Rat lung tissue lysates with 5% non-fat milk/TBS for 1.5 hour at RT. with TBS-0.1% Tween 3 times with 5 minutes each
Lane 2 : Mouse lung tissue lysates with 5% non-fat milk/TBS for 1.5 hour at RT. with TBS-0.1% Tween 3 times with 5 minutes each
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26,35 kDa why is the actual band size different from the predicted?
Exposure time: 3 hoursThe signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody (ab115791)Surfactant Protein A/PSAP was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab115791 overnight at 4°C. Biotinylated goat antirabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody (ab115791)Surfactant Protein A/PSAP was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab115791 overnight at 4°C. Biotinylated goat antirabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab115791 は 14 報の論文で使用されています。
- Li G et al. Surfactant Protein A can Affect the Surface Tension of the Eustachian Tube and Macrophage Migration. Laryngoscope 133:1726-1733 (2023). PubMed: 36102297
- Takahashi T et al. Betamethasone phosphate reduces the efficacy of antenatal steroid therapy and is associated with lower birthweights when administered to pregnant sheep in combination with betamethasone acetate. Am J Obstet Gynecol 226:564.e1-564.e14 (2022). PubMed: 34626553
- Lv MY et al. Complex Evaluation of Surfactant Protein A and D as Biomarkers for the Severity of COPD. Int J Chron Obstruct Pulmon Dis 17:1537-1552 (2022). PubMed: 35811742
- Avci S et al. Relationship of Notch Signal, Surfactant Protein A, and Indomethacin in Cervix During Preterm Birth: Mast Cell and Jagged-2 May Be Key in Understanding Infection-mediated Preterm Birth. J Histochem Cytochem 70:121-138 (2022). PubMed: 34927491
- Takahashi T et al. Single nucleotide polymorphisms in surfactant protein A1 are not associated with a lack of responsiveness to antenatal steroid therapy in a pregnant sheep model. Physiol Rep 10:e15477 (2022). PubMed: 36200269