AB72579

SureLight® APC Anti-6X His tag® 抗体 [AD1.1.10]

Name: SureLight® APC Anti-6X His tag® 抗体 [AD1.1.10] (ab72579) | Abcam
Brand: Abcam Limited
SKU: AB72579
Price: 46000 JPY
Availability: InStock

SureLight® APC Anti-6X His tag® antibody [AD1.1.10]

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(12 Publications)

Anti-6X His tag® antibody [AD1.1.10] - SureLight® APC conjugated (ab72579) is a mouse monoclonal antibody detecting 6X His tag® in Flow Cytometry.

- Trusted since 2008

別名を表示する

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

1 Images

Flow Cytometry - SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (AB72579)

Flow Cyt

Supplier Data

Flow Cytometry - SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (AB72579)

Flow cytometric analysis of transfected HEK293 cells labelling 6X His tag® with ab72579 at 1 µg/mL.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

AD1.1.10

アイソタイプ

IgG1

標識

SureLight® APC

励起波長/蛍光波長

Ex: 652nm, Em: 657nm

キャリアフリー

アプリケーション

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/apc-mouse-fab2-igg1-kappa-monoclonal-15h6-isotype-control-ab37391'>ab37391</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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製品の詳細

What is this antibody validated in?
SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt). Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Related products
Antibody clone AD1.1.10 is also available pre-conjugated to a variety of labels for your convenience – Anti-6X His tag® SureLight® APC [AD1.1.10] (ab72579).

Other related products
We have a range of other formats of antibody clone [AD1.1.10] also available for your convenience: ab15149, PE - ab72467, SureLight® APC - ab72579, Alkaline Phosphatase - ab81652, PerCP - ab117496, Carrier free - ab117504, DyLight® 550 - ab117507, DyLight® 488 - ab117512, HRP - ab178563, Carrier free - ab307236

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Size-exclusion chromatography

精製に関する特記事項

Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.

バッファー組成

pH: 7.4 Preservative: 0.013% Sodium azide Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information 6X His tag®

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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Protein science : a publication of the Protein Society 34:e70072 PubMed39981925

2025

Taming interleukin-12: Engineering of bispecific antibody-based IL-12 mimetics with biased agonism capacities.

Applications

Unspecified application

Species

Unspecified reactive species

Britta Lipinski,Laura Unmuth,Paul Arras,Ron Endruszeit,Stefan Becker,Jonathan Mathias Rödel,Jürgen Scheller,Silke Pudewell,Doreen M Floss,Simon Krah,Julia Harwardt,Achim Doerner,Laura Helming,Chunxiao Xu,Andreas Menrad,Andreas Evers,Harald Kolmar,Desislava Elter,Lukas Pekar,Stefan Zielonka

mAbs 16:2406548 PubMed39304998

2024

Discovery of potent allosteric antibodies inhibiting EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Léxane Fournier,Lukas Pekar,Birgitta Leuthner,Harald Kolmar,Lars Toleikis,Stefan Becker

Nature communications 15:812 PubMed38280880

2024

The cryo-EM structure of homotetrameric attachment glycoprotein from langya henipavirus.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Guo,Songyue Wu,Wenting Li,Haonan Yang,Tianhao Shi,Bin Ju,Zheng Zhang,Renhong Yan

Journal of medical virology 95:e28642 PubMed36890630

2023

Pre-existing immunity to SARS-CoV-2 associates with strong T cell responses induced by inactivated COVID-19 vaccines.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Yi,Yuhao Wang,Quanrun Li,Xiaoyi Li,Panli Zhang,Xin Fu,Shuqin Gu,Daqian Zhang,Xiaoyi Liu,Haonan Lou,Yuemei Wu,Libo Tang,Jinlin Hou,Yongyin Li

iScience 25:104431 PubMed35607524

2022

Structural and functional analysis of an inter-Spike bivalent neutralizing antibody against SARS-CoV-2 variants.

Applications

Unspecified application

Species

Unspecified reactive species

Yaning Li,Qing Fan,Bing Zhou,Yaping Shen,Yuanyuan Zhang,Lin Cheng,Furong Qi,Shuo Song,Yingying Guo,Renhong Yan,Bin Ju,Zheng Zhang

Glycoconjugate journal 39:315-325 PubMed34699015

2021

Structural characteristics of Heparan sulfate required for the binding with the virus processing Enzyme Furin.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin Zeng,Yuan Meng,Shi-Yi Chen,Gaofeng Zhao,Lianchun Wang,En-Xin Zhang,Hong Qiu

Cell reports. Medicine 2:100420 PubMed34604818

2021

Intradermal-delivered DNA vaccine induces durable immunity mediating a reduction in viral load in a rhesus macaque SARS-CoV-2 challenge model.

Applications

Unspecified application

Species

Unspecified reactive species

Ami Patel,Jewell N Walters,Emma L Reuschel,Katherine Schultheis,Elizabeth Parzych,Ebony N Gary,Igor Maricic,Mansi Purwar,Zeena Eblimit,Susanne N Walker,Diana Guimet,Pratik Bhojnagarwala,Opeyemi S Adeniji,Arthur Doan,Ziyang Xu,Dustin Elwood,Sophia M Reeder,Laurent Pessaint,Kevin Y Kim,Anthony Cook,Neethu Chokkalingam,Brad Finneyfrock,Edgar Tello-Ruiz,Alan Dodson,Jihae Choi,Alison Generotti,John Harrison,Nicholas J Tursi,Viviane M Andrade,Yaya Dia,Faraz I Zaidi,Hanne Andersen,Mohamed Abdel-Mohsen,Mark G Lewis,Kar Muthumani,J Joseph Kim,Daniel W Kulp,Laurent M Humeau,Stephanie J Ramos,Trevor R F Smith,David B Weiner,Kate E Broderick

Cell reports 36:109525 PubMed34380042

2021

Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Peter Szodoray,Tor Kristian Andersen,Julia Heinzelbecker,John F Imbery,Peter C Huszthy,Stephanie M Stanford,Bjarne Bogen,Ole B Landsverk,Nunzio Bottini,Anders Tveita,Ludvig A Munthe,Britt Nakken

Nature 584:115-119 PubMed32454513

2020

Human neutralizing antibodies elicited by SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Ju,Qi Zhang,Jiwan Ge,Ruoke Wang,Jing Sun,Xiangyang Ge,Jiazhen Yu,Sisi Shan,Bing Zhou,Shuo Song,Xian Tang,Jinfang Yu,Jun Lan,Jing Yuan,Haiyan Wang,Juanjuan Zhao,Shuye Zhang,Youchun Wang,Xuanling Shi,Lei Liu,Jincun Zhao,Xinquan Wang,Zheng Zhang,Linqi Zhang

eLife 9: PubMed32314731

2020

Landscape mapping of shared antigenic epitopes and their cognate TCRs of tumor-infiltrating T lymphocytes in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kenji Murata,Munehide Nakatsugawa,Muhammed A Rahman,Linh T Nguyen,Douglas G Millar,David T Mulder,Kenji Sugata,Hiroshi Saijo,Yukiko Matsunaga,Yuki Kagoya,Tingxi Guo,Mark Anczurowski,Chung-Hsi Wang,Brian D Burt,Dalam Ly,Kayoko Saso,Alexandra Easson,David P Goldstein,Michael Reedijk,Danny Ghazarian,Trevor J Pugh,Marcus O Butler,Tak W Mak,Pamela S Ohashi,Naoto Hirano

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

SureLight® APC Anti-6X His tag® 抗体 [AD1.1.10]

SureLight® APC Anti-6X His tag® antibody [AD1.1.10]

Flow Cytometry - SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (AB72579)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

アプリケーション

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (12)

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