Anti-Superoxide Dismutase 1 抗体 [2F5] (ab115750)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [2F5] (ab115750)](https://www.abcam.co.jp/ps/products/115/ab115750/Images/ab115750-127875-anti-superoxide-dismutase-1-antibody-2f5-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [2F5] (ab115750)")
Key features and details
- Mouse monoclonal [2F5] to Superoxide Dismutase 1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Superoxide Dismutase 1 antibody [2F5]
Superoxide Dismutase 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2F5] to Superoxide Dismutase 1 -
由来種
Mouse -
特異性
ab115750 does not cross react with immunoglobulins but does react weakly with Mn SOD -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow
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免疫原
Tissue, cells or virus corresponding to Cow Superoxide Dismutase 1. Superoxide dismutase enzyme prepared from bovine erythrocytes
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ポジティブ･コントロール
- Human Kidney and Human Liver tissues
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
2F5 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115750の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Destroys radicals which are normally produced within the cells and which are toxic to biological systems. -
関連疾患
Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. -
配列類似性
Belongs to the Cu-Zn superoxide dismutase family. -
翻訳後修飾
Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. -
細胞内局在
Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria. - Information by UniProt
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参照データベース
- Entrez Gene: 281495 Cow
- Entrez Gene: 6647 Human
- Omim: 147450 Human
- SwissProt: P00442 Cow
- SwissProt: P00441 Human
- Unigene: 443914 Human
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別名
- ALS antibody
- ALS1 antibody
- Amyotrophic lateral sclerosis 1 adult antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [2F5] (ab115750)Human Kidney (formalin-fixed, paraffin-embedded) stained with ab115750 at 15 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [2F5] (ab115750)](https://www.abcam.co.jp/ps/products/115/ab115750/Images/ab115750-127874-anti-superoxide-dismutase-1-antibody-2f5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [2F5] (ab115750)Human Liver (formalin-fixed, paraffin-embedded) stained with ab115750 at 15 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab115750 は論文での使用が確認できていません。