Name: Anti-Sumo 2 + Sumo 3 antibody [8A2]
SKU: ab81371
Rating: 4 (7 reviews)

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Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [8A2] to Sumo 2 + Sumo 3
Suitable for: WB, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

製品名

Anti-Sumo 2 + Sumo 3 antibody [8A2]
Sumo 2 + Sumo 3 一次抗体製品一覧
製品の詳細

Mouse monoclonal [8A2] to Sumo 2 + Sumo 3
由来種

Mouse
特異性

This antibody recognizes Sumo 2 and 3 but does not recognize Sumo 1.
アプリケーション

適用あり: WB, ICC/IF, IHC-Pmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Recombinant full length protein corresponding to Human Sumo 2 + Sumo 3.
Database link: P61956, P55854
ポジティブ･コントロール
- WB: HeLa, SH-SY5Y, K562, SW480, Jurkat, HEK-293T, PC-12 and NIH/3T3 whole cell lysate. Human brain, spleen and kidney tissue lysate. Rat spleen tissue lysate. Mouse brain tissue lysate. IHC-P: Mouse and rat kidney tissue. Human breast tissue. ICC: HeLa, SW480 and NIH/3T3 cells.
特記事項
This product has switched from ascites to recombinant production method on 25^th August 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
バッファー

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

8A2
アイソタイプ

IgG2b
研究分野

Alternative Versions
- Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)
ChIP Related Products
- ChIP Kit (ab500)
Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
Related Products

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab81371の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(3)	1/1000. Predicted molecular weight: 12 kDa.
ICC/IF	(2)	1/2000.
IHC-P		1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

特記事項
WB 1/1000. Predicted molecular weight: 12 kDa.
ICC/IF 1/2000.
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

関連性

SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
細胞内局在

Cytoplasmic (SUMO3) and Nuclear (SUMO2)
参照データベース
- Entrez Gene: 6612 Human
- Entrez Gene: 6613 Human
- Entrez Gene: 170930 Mouse
- Entrez Gene: 20610 Mouse
- Entrez Gene: 499417 Rat
- Entrez Gene: 690244 Rat
- Omim: 602231 Human
- Omim: 603042 Human
- SwissProt: P55854 Human
- SwissProt: P61956 Human
- SwissProt: P61957 Mouse
- SwissProt: Q9Z172 Mouse
- SwissProt: P61959 Rat
- SwissProt: Q5XIF4 Rat
see all
別名
- HSMT3 antibody
- MGC117191 antibody
- OTTHUMP00000115275 antibody
- OTTHUMP00000115276 antibody
- OTTHUMP00000115277 antibody
- Sentrin 2 antibody
- Small ubiquitin like modifier 2 antibody
- Small ubiquitin like modifier 3 antibody
- Small ubiquitin related modifier 2 antibody
- Small ubiquitin related modifier 3 antibody
- small ubiquitin-like modifier 3 antibody
- small ubiquitin-related modifier 3 antibody
- SMT3 homolog 1 antibody
- SMT3 homolog 2 antibody
- SMT3 suppressor of mif two 3 homolog 1 antibody
- SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody
- SMT3 suppressor of mif two 3 homolog 2 antibody
- SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) antibody
- SMT3 suppressor of mif two 3 homolog 3 antibody
- SMT3A antibody
- SMT3B antibody
- SMT3H1 antibody
- SMT3H2 antibody
- Sumo2 antibody
- Sumo3 antibody
- Ubiquitin like protein SMT3A antibody
- Ubiquitin like protein SMT3B antibody
- Ubiquitin-like protein SMT3B antibody
see all

Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

All lanes : Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate B276
Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 4 : SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 6 : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 7 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 8 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L)

Predicted band size: 12 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?

Buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).
Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

All lanes : Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371) at 1/1000 dilution

Lane 1 : Human brain tissue lysate
Lane 2 : Human kidney tissue lysate
Lane 3 : Human spleen tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Rat spleen tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 12 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?

Dilution and concentration: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

Immunocytochemistry/immunofluorescence analysis of NIH/3T3 cells labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit. secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

Immunocytochemistry/immunofluorescence analysis of HeLa cells labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit. secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).
Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

Immunocytochemistry/immunofluorescence analysis of SW480 cell line labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit. secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).
Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371)

ChIP protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab81371 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab81371 は 69 報の論文で使用されています。

G GK et al. Understanding the Combined Effects of High Glucose Induced Hyper-Osmotic Stress and Oxygen Tension in the Progression of Tumourigenesis: From Mechanism to Anti-Cancer Therapeutics. Cells 12:N/A (2023). PubMed: 36980166
van den Berg SJW et al. p97/VCP drives turnover of SUMOylated centromeric CCAN proteins and CENP-A. Mol Biol Cell 34:br6 (2023). PubMed: 36989032
Suk TR et al. Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain. iScience 26:106350 (2023). PubMed: 37009224
Hofmann S et al. SUMO Modification of Hepatitis B Virus Core Mediates Nuclear Entry, Promyelocytic Leukemia Nuclear Body Association, and Efficient Formation of Covalently Closed Circular DNA. Microbiol Spectr 11:e0044623 (2023). PubMed: 37199632
Qian L et al. Cellular gp96 upregulates AFP expression by blocking NR5A2 SUMOylation and ubiquitination in hepatocellular carcinoma. J Mol Cell Biol 15:N/A (2023). PubMed: 37204028

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-10 of 12 Abreviews or Q&A

Application

Immunoprecipitation

Sample

Human Cell lysate - whole cell (A549)

Total protein in input

4e+006 cells

Immuno-precipitation step

Protein G

Specification

A549

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Marie Lork

Verified customer

投稿 Jun 23 2022

Application

Western blot

Sample

Human Cell lysate - whole cell (breast cancer cell lines)

Gel Running Conditions

Reduced Denaturing

Loading amount

10 µg

Specification

breast cancer cell lines

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 01 2016

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

Serum as blocking agent for 10 minute(s) · Concentration: 0.1% · Temperature: 25°C

Sample

Rat Cell (Glial cells)

Specification

Glial cells

Permeabilization

Yes - 0.01% Saponin

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 10 2014

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (12%)

Sample

Rat Cell lysate - whole cell (Mixed glial cells)

Specification

Mixed glial cells

Blocking step

BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 30 2014

Application

Western blot

Sample

Xenopus laevis Cell lysate - whole cell (Xenopus egg extract)

Loading amount

40 µg

Specification

Xenopus egg extract

Gel Running Conditions

Reduced Denaturing (15%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. XXXXXX XXXXXX

Verified customer

投稿 Apr 11 2013

Application

Immunocytochemistry/ Immunofluorescence

Sample

Xenopus laevis Cell (Nuclei generated in oocyte extract)

Specification

Nuclei generated in oocyte extract

Fixative

Formaldehyde

Permeabilization

Yes - PBS-0.1% NP40

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. XXXXXX XXXXXX

Verified customer

投稿 Apr 01 2013

Application

ChIP

Sample

Human Cell lysate - whole cell (liver)

Specification

liver

Type

Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37%FORMALDEHYDE

Detection step

Semiquantitative PCR

Positive control

pol 11

Negative control

IgG

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Natarajan Balasubramaniyan

Verified customer

投稿 May 19 2012

Question

I had ever ordered and used your antibody product which was the ab81371 mouse Anti-Sumo 2+3 antibody [8A2] - ChIP Grade (Lot: GR57044-5), but it seemed that there was a cross reaction with my protein of interest, human eIF4G, from western blotting. However, I would still like to buy another one from your company. Is it possible that you will give me some aliqout of anti-sumo 2+3 antibodies you have in stock right now in order to trial the cross reactio with the protein?
Thank you in advance,

Abcam community

Verified customer

Asked on Jun 27 2013

Answer

Thank you for contacting us. Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 12 months of purchase.

So my suggestion would be to try the antibody and if not completely satisfied we will either replace the antibody or give you a different product to try.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Padamjeet Singh

Abcam Scientific Support

Answered on Jun 27 2013

Question

can you BLAST the immunogen with the mouse sequence?

Abcam community

Verified customer

Asked on Mar 19 2013

Answer

Thank you for contacting us.
Both ab3742 and ab81371 have immunogen with 100% homology with the mouse sequence.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

Answered on Mar 19 2013

Question

Inquiry: We have purchased SUMO2+3 antibody (8A2) (cat.No: ab81371, Lot No: 100028-1). Could you please tell us the concentration of this antibody? I need this information in order to carry out IP. Another question is what is the difference between ChIP Grade antibody or not? Thank you very much in advance.

Abcam community

Verified customer

Asked on Nov 02 2012

Answer

Thank you for your enquiry and your interest in our products.
This particular antibody is not affinity purified but ascitic fluid so the concentration is not determined. As a general guidance, the concentration range is between: 5 - 10 mg/ml and the recommended dilution factor for IP would be 1/100. You may wish to take a look at this Table for further details:
https://www.abcam.com/index.html?pageconfig=technicalfaqs#8
If an antibody is ChIP grade, it means that it has been specifically tested in ChIP application in the Lab or we have specific published data available for the product so is suitable for this application.
If you need any further assistance in the future, please do not hesitate to contact me.

Abcam Scientific Support

Answered on Nov 02 2012

1-10 of 12 Abreviews or Q&A

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

ChIP Related Products

Compatible Secondaries

Isotype control

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

関連性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (69)

レビューと Q&A

Immunoprecipitation abreview for Anti-Sumo 2 + Sumo 3 antibody [8A2]

Western blot abreview for Anti-Sumo 2+3 antibody [8A2] - ChIP Grade

Immunocytochemistry/ Immunofluorescence abreview for Anti-Sumo 2+3 antibody [8A2] - ChIP Grade

Western blot abreview for Anti-Sumo 2+3 antibody [8A2] - ChIP Grade

Western blot abreview for Anti-Sumo 2+3 antibody [8A2] - ChIP Grade

Immunocytochemistry/ Immunofluorescence abreview for Anti-Sumo 2+3 antibody [8A2] - ChIP Grade

ChIP abreview for Anti-Sumo 2+3 antibody [8A2] - ChIP Grade