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AB179907

Biotin Anti-Sumo 1 抗体 [4D12]

Biotin Anti-Sumo 1 antibody [4D12]

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(2 Publications)

Rat Monoclonal Sumo 1 antibody - conjugated to Biotin. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SUMO1.

別名を表示する

SMT3C, SMT3H3, UBL1, OK/SW-cl.43, SUMO1, Small ubiquitin-related modifier 1, SUMO-1, GAP-modifying protein 1, SMT3 homolog 3, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1, GMP1, Smt3C

2 Images
Immunocytochemistry/ Immunofluorescence - Biotin Anti-Sumo 1 antibody [4D12] (AB179907)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Biotin Anti-Sumo 1 antibody [4D12] (AB179907)

Immunofluorescent analysis of C-33A Human cervix carcinoma cells labeling Sumo 1 with ab179907 at 10 ug/ml

Western blot - Biotin Anti-Sumo 1 antibody [4D12] (AB179907)
  • WB

Supplier Data

Western blot - Biotin Anti-Sumo 1 antibody [4D12] (AB179907)

An 80 kDa single and other multiple bands were observed in HeLa total cell extract at 1 ug/ml

All lanes:

Western blot - Biotin Anti-Sumo 1 antibody [4D12] (ab179907)

All lanes:

HeLa total cell extract

Predicted band size: 11 kDa

false

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

4D12

アイソタイプ

IgG2a

軽鎖のタイプ

kappa

標識

Biotin

励起波長/蛍光波長
キャリアフリー

No

交差種

Mouse, Human

アプリケーション

WB, ICC/IF

applications

免疫原

Recombinant Full Length Protein corresponding to Human SUMO1.

P63165

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Proprietary technique
精製に関する特記事項
ab179907 was produced in serum-free medium and purified under mild conditions by propriety chromatography processes and conjugated with biotin. ab179907 is filter-sterilised.
バッファー組成
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SUMO 1 also known as small ubiquitin-like modifier 1 is part of the protein family involved in post-translational modification. The protein mechanically adds a SUMO group to target proteins through sumoylation a process similar to ubiquitination. SUMO 1 typically has a molecular mass of around 11 kDa. It expresses in various tissues and cells including the nucleus and cytoplasm of eukaryotic cells. In addition it is important in processes like nuclear transport transcriptional regulation and protein stabilization.
Biological function summary

SUMO 1 contributes significantly to maintaining cellular homeostasis. It is an integral part of a SUMOylation complex that modifies other proteins to alter their function localization or interactions. Through its modification actions SUMO 1 affects processes such as DNA repair and the cell cycle. By interacting with components of the nuclear pore complex and transcription factors SUMO 1 modulates essential biological activities at multiple levels within the cell.

Pathways

This protein plays a significant role in the PI3K/Akt pathway and Wnt signaling. SUMO 1 associates with proteins like RanGAP1 and various transcription factors highlighting the complexity of its regulatory actions. These associations are important for the regulation of cell survival proliferation and differentiation. SUMOylation by SUMO 1 has been linked to an interference with phosphorylation showing its potential influence on cell signaling pathways.

The dysregulation of SUMO 1 is seen in cancer and neurodegenerative diseases. For instance altered SUMOylation patterns contribute to the progression of certain cancers. Furthermore SUMO 1 interacts with proteins like p53 and these interactions can affect tumor suppression and cell cycle regulation. In neurodegenerative disorders disturbed SUMOylation is associated with protein aggregation and neuronal damage implicating SUMO 1 in diseases like Alzheimer's.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1 (PubMed : 19223394). Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (PubMed : 24651376).
See full target information SUMO1
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 281:23180-90 PubMed16757475

2006

Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhiro Uchimura,Takaya Ichimura,Junsuke Uwada,Taro Tachibana,Satoko Sugahara,Mitsuyoshi Nakao,Hisato Saitoh

Experimental cell research 312:1418-30 PubMed16688858

2006

In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies.

Applications

Unspecified application

Species

Unspecified reactive species

Noriko Saitoh,Yasuhiro Uchimura,Taro Tachibana,Satoko Sugahara,Hisato Saitoh,Mitsuyoshi Nakao
View all publications
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代替製品

Primary Antibodies

AB5316

Anti-Sumo 1 antibody

Western blot - Anti-Sumo 1 antibody (AB5316)

  • 4
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Primary Antibodies

AB196533

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Primary Antibodies

AB133352

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20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (AB133352)

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  • 2
Primary Antibodies

AB32058

Anti-Sumo 1 antibody [Y299] - ChIP Grade

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (AB32058)

  • 4
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Primary Antibodies

AB219724

Anti-Sumo 1 antibody [Y299] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - BSA and Azide free (AB219724)

  • 0
  • 0
Primary Antibodies

AB196310

Alexa Fluor® 488 Anti-Sumo 1 antibody [Y299]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Sumo 1 antibody [Y299] (AB196310)

  • 0
  • 0

組合せ製品

Assay Kits

AB139470

SUMOylation Assay Kit

Functional Studies - SUMOylation Assay Kit (AB139470)

  • 0
  • 0
Primary Antibodies

AB81371

Anti-Sumo 2 + Sumo 3 antibody [8A2]

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] (AB81371)

  • 4
  • 7
Proteins & Peptides

AB140417

Recombinant Human Sumo 1 protein

Western blot - Recombinant Human Sumo 1 protein (AB140417)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB51254

Anti-Superoxide Dismutase 1 antibody [EP1727Y]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (AB51254)

  • 5
  • 4

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