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遺伝子ノックアウト細胞株 検証済リコンビナント

Anti-SUFU 抗体 [1783CT536.263.29] (ab302679)

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Western blot - Anti-SUFU antibody [1783CT536.263.29] (AB302679)
  • Western blot - Anti-SUFU antibody [1783CT536.263.29] (AB302679)
  • Anti-SUFU antibody [1783CT536.263.29] (AB302679)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [1783CT536.263.29] to SUFU
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-SUFU antibody [1783CT536.263.29]
    SUFU 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [1783CT536.263.29] to SUFU

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WBmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    The details of the immunogen for this antibody are not available.

  • ポジティブ･コントロール

    • WB: Wild type and SUFU knockout HAP1, whole cell lysates; HeLa and 293T whole cell lysates; human testis tissue lysate.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302679の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
特記事項
WB
1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Negative regulator in the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes (PubMed:15367681, PubMed:24311597, PubMed:24217340). Down-regulates GLI2-mediated transactivation of target genes (PubMed:24311597, PubMed:24217340). Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein (PubMed:10806483, PubMed:24217340). Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Required for normal embryonic development. Required for the proper formation of hair follicles and the control of epidermal differentiation.

  • 組織特異性

    Ubiquitous in adult tissues. Detected in osteoblasts of the perichondrium in the developing limb of 12-week old embryos. Isoform 1 is detected in fetal brain, lung, kidney and testis. Isoform 2 is detected in fetal testis, and at much lower levels in fetal brain, lung and kidney.

  • 関連疾患

    Medulloblastoma

  • 配列類似性

    Belongs to the SUFU family.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • OTTHUMP00000020374 antibody
      • OTTHUMP00000020377 antibody
      • OTTHUMP00000020379 antibody
      • PRO1280 antibody
      • SU FU antibody
      • SU(F)U antibody
      • Su(fu) antibody
      • SUFU antibody
      • SUFU negative regulator of hedgehog signaling antibody
      • SUFU_HUMAN antibody
      • SUFUH antibody
      • SUFUXL antibody
      • Suppressor of Fused antibody
      • Suppressor of fused homolog (Drosophila) antibody
      • Suppressor of fused homolog antibody
      see all

    画像

    • Western blot - Anti-SUFU antibody [1783CT536.263.29] (AB302679)
      Western blot - Anti-SUFU antibody [1783CT536.263.29] (AB302679)
      All lanes : Anti-SUFU antibody [1783CT536.263.29] (ab302679) at 1/1000 dilution

      Lane 1 : Wild type HAP1, whole cell lysate
      Lane 2 : SUFU knockout HAP1, whole cell lysate
      Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
      Lane 4 : 293T (human embryonic kidney epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (IRDye® 800CW) (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (ab216777) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 53 kDa
      Observed band size: 53 kDa



      Blocking / Diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.

      False colour image of Western blot: Anti-SUFU antibody [1783CT536.263.29] (ab302679) staining at 1/1000 dilution, shown in green; Rabbit anti-GAPDH antibody [16891] (ab181602) loading control staining at 1/20000 dilution, shown in red.

      In Western blot, ab302679 was shown to bind specifically to SUFU. A band was observed at 47 kDa in wild-type HAP1 cell lysates with no signal observed at this size in the SUFU knockout cell line. To generate this image, wild-type and SUFU knockout HAP1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a PVDF-FL membrane. Membranes were blocked in Odyssey diluted in equal volume of 0.1 % TBS before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Mouse IgG H&L (IRDye® 800CW) (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (ab216777) at 1/10000 dilution.

      The identity of the bands beneath the target band are unknown.

    • Western blot - Anti-SUFU antibody [1783CT536.263.29] (AB302679)
      Western blot - Anti-SUFU antibody [1783CT536.263.29] (AB302679)
      Anti-SUFU antibody [1783CT536.263.29] (ab302679) at 1/1000 dilution + Human testis tissue lysate at 20 µg

      Secondary
      Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Predicted band size: 53 kDa
      Observed band size: 53 kDa


      Exposure time: 3 minutes


      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

      The identity of the band beneath the target band is unknown.

    • Anti-SUFU antibody [1783CT536.263.29] (AB302679)
      Anti-SUFU antibody [1783CT536.263.29] (AB302679)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302679 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302679 は論文での使用が確認できていません。

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