Anti-SUCLG2 抗体 (ab187996)
Key features and details
- Rabbit polyclonal to SUCLG2
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SUCLG2 antibody
SUCLG2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SUCLG2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICCmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Pig
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免疫原
Recombinant fragment corresponding to Human SUCLG2 aa 350 to the C-terminus.
Database link: Q96I99 -
ポジティブ･コントロール
- IHC-P: Human testis, pancreas, liver, and kidney tissues; ICC: HepG2 cells; WB: U-87 MG and SCLC-21H cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187996の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 47 kDa.
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| IHC-P |
1/5000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC |
Use a concentration of 0.25 - 2 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 47 kDa. |
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IHC-P
1/5000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC
Use a concentration of 0.25 - 2 µg/ml. |
ターゲット情報
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機能
Catalyzes the GTP-dependent ligation of succinate and CoA to form succinyl-CoA. -
組織特異性
Mainly expressed in liver, kidney, heart, spleen and skeletal muscle. Also found in intestine and colon, and in low amounts in lung, brain, prostate, testis and ovary. -
パスウェイ
Carbohydrate metabolism; tricarboxylic acid cycle. -
配列類似性
Belongs to the succinate/malate CoA ligase beta subunit family.
Contains 1 ATP-grasp domain. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 8801 Human
- Entrez Gene: 20917 Mouse
- Entrez Gene: 397026 Pig
- Omim: 603922 Human
- SwissProt: Q96I99 Human
- SwissProt: Q9Z2I8 Mouse
- SwissProt: P53590 Pig
- Unigene: 644919 Human
see all -
別名
- EC 6.2.1.4 antibody
- G BETA antibody
- GBETA antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG2 antibody (ab187996)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SUCLG2 with ab187996 at 1/5000 dilution.
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Immunocytochemistry - Anti-SUCLG2 antibody (ab187996)Immunofluorescent analysis of PFA/Triton X-100-treated HepG2 cells labeling SUCLG2 with ab187996 at 4 µg/ml.
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Western blot - Anti-SUCLG2 antibody (ab187996)All lanes : Anti-SUCLG2 antibody (ab187996) at 0.4 µg/ml
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 2 : SCLC-21H (human small cell lung carcinoma cell line) cell lysate
Predicted band size: 47 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG2 antibody (ab187996)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling SUCLG2 with ab187996 at 1/5000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG2 antibody (ab187996)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SUCLG2 with ab187996 at 1/5000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG2 antibody (ab187996)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SUCLG2 with ab187996 at 1/5000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab187996 は 2 報の論文で使用されています。
- Li B et al. Overexpression of PTEN may increase the effect of pemetrexed on A549 cells via inhibition of the PI3K/AKT/mTOR pathway and carbohydrate metabolism. Mol Med Rep 20:3793-3801 (2019). PubMed: 31485626
- Wongkittichote P et al. Tricarboxylic acid cycle enzyme activities in a mouse model of methylmalonic aciduria. Mol Genet Metab 128:444-451 (2019). PubMed: 31648943