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AB322069

Anti-Succinic cysteine 抗体 [EPR28566-70] - BSA and Azide free

Anti-Succinic cysteine antibody [EPR28566-70] - BSA and Azide free

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Rabbit Recombinant Monoclonal s-(2-Succinyl)cysteine antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples.
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Competitive ELISA - Anti-Succinic cysteine antibody [EPR28566-70] - BSA and Azide free (AB322069)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Succinic cysteine antibody [EPR28566-70] - BSA and Azide free (AB322069)

ab322069 (0.1 ug/mL) is specific to Succinic cysteine and the percent cross reactivity with other compounds is demonstrated in the table.

Competitive ELISA - Anti-Succinic cysteine antibody [EPR28566-70] - BSA and Azide free (AB322069)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Succinic cysteine antibody [EPR28566-70] - BSA and Azide free (AB322069)

4 ug/mL Succinic cysteine-crosslink-BSA was coated onto 96-wells. Serial dilution of free Succinic cysteine (0, 195.3125, 390.625, 781.25, 1562.5, 3125, 6250, 12500, 25000, 50000 ng/mL) was added (50 uL). This was followed by adding 0.1 ug/mL ab322069 (50 uL) into each well. The plate was incubated for 30 mins. After washing, 1 : 20000 diluted GAR Fc-HRP was added and incubated for 30 mins. After washing, 100 uL of TMB was added and incubated for 5-10 mins without shaking for color development. Then 100 uL of stop solution (4.9% Orthophosphoric acid) was dispensed and OD was read at 450nm on a microplate reader within 5-10 mins.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR28566-70

アイソタイプ

IgG

キャリアフリー

Yes

アプリケーション

cELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This antibody has 0.4345% cross reaction with Succinic acid, but does not cross-react with L-Cysteine.

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Reactivity data

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製品の詳細

ab322069 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for competitive ELISAs to quantify Succinic cysteine.

The reference range value is 195.31-50000 ng/mL.

Please note that the range provided for this antibody is only an estimation. Performance of the antibody depends on the specific characteristics of your assay. We guarantee the product works in competitive ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Succinic cysteine also known as S-cysteinylsuccinic acid is a modified amino acid and a derivative of succinic acid. It forms through the addition of a succinyl group to cysteine. The molecular mass of succinic cysteine is approximately 193.22 g/mol. It primarily accumulates in the mitochondria due to its involvement in metabolic processes. Succinic cysteine does not have expression in tissues or cellular compartments like conventional proteins but is more of a biochemical entity.
Biological function summary

The presence of succinic cysteine in cells indicates interactions with the sulfur-containing amino acid cysteine. It participates in various biochemical processes particularly those related to amino acid metabolism and antioxidant defense. It is not a component of any complex but interacts with the glutathione metabolic pathway. It can potentially act as an intermediate in reactions involving redox changes impacting cellular responses under oxidative stress.

Pathways

The presence and formation of succinic cysteine have roles in amino acid metabolism and the tricarboxylic acid (TCA) cycle. It connects to glutathione and can influence its synthesis and utilization within these pathways. This link extends to other proteins in the glutathione pathway like glutathione synthetase and gamma-glutamylcysteine synthetase which regulate redox balance in cells.

Alterations in succinic cysteine levels may play roles in oxidative stress-related conditions and mitochondrial disorders. Abnormal levels influence diseases like mitochondrial encephalomyopathies where improper metabolism of intermediates like succinic cysteine affects energy production in cells. It may also have associations with conditions that involve glutathione-related oxidative stress connecting indirectly to proteins like superoxide dismutase that manage oxidative stress responses in various biological contexts.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

See full target information s-(2-Succinyl)cysteine
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