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AB316119

Anti-STX17 抗体 [EPR28622-84]

Anti-STX17 antibody [EPR28622-84]

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(1 Publication)

Knockout Tested Rabbit Recombinant Monoclonal STX17 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

別名を表示する

Syntaxin-17, STX17

3 Images
Western blot - Anti-STX17 antibody [EPR28622-84] (AB316119)
  • WB

Supplier Data

Western blot - Anti-STX17 antibody [EPR28622-84] (AB316119)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the band between 75 kDa and 100 kDa in lane 1-5 is unknown.

All lanes:

Western blot - Anti-STX17 antibody [EPR28622-84] (ab316119) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Lane 5:

Mouse adrenal gland tissue lysate at 20 µg

Lane 6:

Rat testis tissue lysate at 20 µg

Lane 7:

Rat adrenal gland tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 33 kDa

false

Exposure time: 180s

Western blot - Anti-STX17 antibody [EPR28622-84] (AB316119)
  • WB

Supplier Data

Western blot - Anti-STX17 antibody [EPR28622-84] (AB316119)

In Western blot, ab316119 was shown to bind specifically to STX17.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Target of interest was observed at 33 KD in wild-type Hela cell lysates (lane 1, wild-type cell line ab255928) with no signal observed at this size in STX17 knockout cell line (lane 2, knockout cell line ab265909/knockout cell lysate ab259167).

The identity of the band around 100 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-STX17 antibody [EPR28622-84] (ab316119) at 1/1000 dilution

Lane 1:

Wild-type Hela (human cervical adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 2:

STX17 knockout Hela whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 33 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-STX17 antibody [EPR28622-84] (AB316119)
  • WB

Supplier Data

Western blot - Anti-STX17 antibody [EPR28622-84] (AB316119)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands between 50 kDa and 100 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-STX17 antibody [EPR28622-84] (ab316119) at 1/1000 dilution

Lane 1:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 2:

Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 33 kDa,36 kDa

false

Exposure time: 180s

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-STX17 antibody [EPR28622-84] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR28622-84

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Mouse, Rat

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

STX17 also known as Syntaxin 17 is a SNARE protein involved in membrane fusion processes. Its molecular weight is approximately 34 kDa. STX17 is predominantly expressed in the endoplasmic reticulum and associated membranes. The protein plays a mechanical role in mediating the fusion of vesicles with target membranes facilitating the transport of molecules within cells. STX17 localizes mostly in cell types associated with high metabolic activity where it supports cellular trafficking demands.
Biological function summary

Syntaxin 17 contributes to the formation of a multiprotein complex involved in the fusion of autophagosomes with lysosomes. This process is important for macroautophagy where damaged organelles and proteins are degraded and recycled. The protein forms a functional unit with other SNARE proteins including VAMP8 and SNAP29 to carry out its role. It ensures cellular homeostasis by facilitating the clearance of unnecessary or damaged cellular components under stress conditions.

Pathways

Syntaxin 17 is critical in the autophagy and endocytosis pathways. These pathways are involved in the degradation and recycling of cellular components maintaining cellular integrity and energy balance. In the autophagy pathway STX17 functions alongside proteins such as LC3 and Beclin-1 coordinating the recognition and processing of autophagosomes. In the endocytosis pathway its interactions help balance the intake and processing of extracellular materials.

STX17 shows significant connections to neurodegenerative disorders and cancer. Its dysfunction in the autophagy process may lead to accumulation of toxic protein aggregates contributing to disorders like Alzheimer's disease. Defects or mutations in the functioning of STX17 have been linked to impaired cellular waste disposal in this context. In cancer alterations in STX17 expression or function can disrupt autophagic processes and affect cancer cell survival. Understanding its link with neurodegenerative proteins such as tau could offer insights for therapeutic strategies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion (PubMed : 23217709, PubMed : 25686604, PubMed : 28306502). STX17 is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane (PubMed : 23217709, PubMed : 25686604, PubMed : 28306502, PubMed : 28504273). May also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi (PubMed : 21545355).
See full target information STX17
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 13:e70002 PubMed39497621

2024

A switch from lysosomal degradation to secretory autophagy initiates osteogenic bone metastasis in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Wei,Mengmeng Liang,Min Deng,Ji Zheng,Fei Luo,Qinyu Ma
View all publications
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