Anti-Sts1 抗体 (ab34781)
Key features and details
- Rabbit polyclonal to Sts1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Sts1 antibody
Sts1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Sts1 -
由来種
Rabbit -
特異性
A BLAST analysis was used to suggest reactivity with this protein from mouse, human, rat and dog based on 100% homology for the immunogen sequence. Cross reactivity with Sts-1 homologues from other sources has not been determined.
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アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Dog
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免疫原
Synthetic peptide corresponding to the C-terminus of mouse Sts-1.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This affinity purified antibody is directed against mouse Sts-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34781の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
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IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination. -
配列類似性
Contains 1 SH3 domain.
Contains 1 UBA domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 84959 Human
- Entrez Gene: 72828 Mouse
- Entrez Gene: 315579 Rat
- Omim: 609201 Human
- SwissProt: Q8TF42 Human
- SwissProt: Q8BGG7 Mouse
- Unigene: 444075 Human
- Unigene: 133615 Mouse
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別名
- Cbl interacting protein p70 antibody
- Cbl interacting protein Sts 1 antibody
- Cbl interacting protein Sts1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sts1 antibody (ab34781)
Immunohistochemical analysis of Formalin fixed/paraffin embedded human tonsil sections labeling Sts-1 with ab34781 at 4 µg/mL. A: Negative control, B: staining of human tonsil at 20X and C: staining of human tonsil at 40X. Sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3'3-diamino-benzidine substrate (DAB).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab34781 は 7 報の論文で使用されています。
- Ciuffa R et al. Novel biochemical, structural, and systems insights into inflammatory signaling revealed by contextual interaction proteomics. Proc Natl Acad Sci U S A 119:e2117175119 (2022). PubMed: 36179048
- Ke M et al. Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics. Nat Commun 12:71 (2021). PubMed: 33397984
- Nguyen TTT et al. Cbl-b deficiency prevents functional but not phenotypic T cell anergy. J Exp Med 218:N/A (2021). PubMed: 33974042
- Tang BX et al. Ziyin Bushen Decoction Alleviates Perimenopausal Syndrome in Rats by Enhancing Estradiol Production. Evid Based Complement Alternat Med 2020:8895809 (2020). PubMed: 33204296
- Feng Y et al. Hepatic steroid sulfatase critically determines estrogenic activities of conjugated equine estrogens in human cells in vitro and in mice. J Biol Chem 294:12112-12121 (2019). PubMed: 31217279
- Hu H et al. Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination. J Exp Med 213:399-414 (2016). WB ; Mouse . PubMed: 26903241
- Yang M et al. K33-linked polyubiquitination of Zap70 by Nrdp1 controls CD8(+) T cell activation. Nat Immunol 16:1253-62 (2015). WB . PubMed: 26390156