Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Stromal interaction molecule 1 抗体 [EPR3414] (ab108994)

Reviews (1) Submit a question References (22)
Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
  • Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
  • Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3414] to Stromal interaction molecule 1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

製品の概要

  • 製品名

    Anti-Stromal interaction molecule 1 antibody [EPR3414]
    Stromal interaction molecule 1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3414] to Stromal interaction molecule 1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt or ICC/IF

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • SH SY5Y, K562, HepG2, HeLa and LnCaP cell lysates; Human breast tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108994の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval with citrate buffer (pH 6): Heat up to 98°C, below boiling, and then let cool for 10-20 min.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval with citrate buffer (pH 6): Heat up to 98°C, below boiling, and then let cool for 10-20 min.
追加情報
Is unsuitable for Flow Cyt or ICC/IF.

ターゲット情報

  • 機能

    Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.

  • 組織特異性

    Ubiquitously expressed in various human primary cells and tumor cell lines.

  • 関連疾患

    Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition.

  • 配列類似性

    Contains 1 EF-hand domain.
    Contains 1 SAM (sterile alpha motif) domain.

  • ドメイン

    The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.

  • 翻訳後修飾

    Glycosylation is required for cell surface expression.
    Phosphorylated predominantly on Ser residues.

  • 細胞内局在

    Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • D11S4896E antibody
    • GOK antibody
    • OTTHUMP00000164512 antibody
    • OTTHUMP00000229140 antibody
    • OTTHUMP00000230742 antibody
    • SIM antibody
    • STIM 1 antibody
    • STIM1 antibody
    • Stim1 stromal interaction molecule 1 antibody
    • STIM1_HUMAN antibody
    • STIM1L antibody
    • Stromal interaction molecule 1 antibody
    see all

画像

  • Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
    Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
    All lanes : Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : STIM1 (Stromal interaction molecule 1) knockout HAP1 whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 77 kDa
    Observed band size: 77 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab108994 observed at 77 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab108994 was shown to specifically react with Stromal interaction molecule 1 in wild-type HAP1 cells as signal was lost in STIM1 (Stromal interaction molecule 1) knockout cells. Wild-type and STIM1 (Stromal interaction molecule 1) knockout samples were subjected to SDS-PAGE. Ab108994 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)

    ab108994 at 1/50 dilution, staining Stromal interaction molecule 1 in Human breast by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
    Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
    All lanes : Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994) at 1/1000 dilution

    Lane 1 : SH SY5Y cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : LnCaP cell lysate

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 77 kDa

  • Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
    Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)

参考文献 (22)

ab108994 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108994 は 22 報の論文で使用されています。

  • Zhou W  et al. IGF2BP2‑dependent STIM1 inhibition protects against LPS‑induced pneumonia in vitro by alleviating endoplasmic reticulum stress and the inflammatory response. Exp Ther Med 26:575 (2023). PubMed: 38023363
  • Tanwar J  et al. MITF is a novel transcriptional regulator of the calcium sensor STIM1: Significance in physiological melanogenesis. J Biol Chem 298:102681 (2022). PubMed: 36356899
  • Manno C  et al. Quantification of the calcium signaling deficit in muscles devoid of triadin. PLoS One 17:e0264146 (2022). PubMed: 35213584
  • Li M  et al. Intracellular Ca2+ Signaling and Calcium Release-Activated Calcium Modulator ORAI1 Are Associated With CD4+ T Lymphocytes in Dairy Cows. Front Immunol 13:835936 (2022). PubMed: 35663942
  • Zheng CB  et al. Ang II Promotes Cardiac Autophagy and Hypertrophy via Orai1/STIM1. Front Pharmacol 12:622774 (2021). PubMed: 34079454
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

Immunocytochemistry/ Immunofluorescence abreview for Anti-Stromal interaction molecule 1 antibody [EPR3414]

 Excellent
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

投稿 Oct 07 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com