Anti-Stromal interaction molecule 1 抗体 (ab62031)
Key features and details
- Rabbit polyclonal to Stromal interaction molecule 1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-Stromal interaction molecule 1 antibody
Stromal interaction molecule 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Stromal interaction molecule 1 -
由来種
Rabbit -
特異性
ab62031 detects the larger isoform of Stromal interaction molecule 1 only. It is predicted to have no cross-reactivity with Stromal interaction molecule 2. -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse -
免疫原
Raised against a 24 amino acid synthetic peptide from near the carboxy terminus of Human Stromal interaction molecule 1 (GenBank accession no. NP_003147).
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ポジティブ･コントロール
- Mouse thymus tissue lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62031の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 77 kDa).
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| IHC-P |
Use a concentration of 2.5 µg/ml.
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| 特記事項 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 77 kDa). |
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IHC-P
Use a concentration of 2.5 µg/ml. |
ターゲット情報
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機能
Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. -
組織特異性
Ubiquitously expressed in various human primary cells and tumor cell lines. -
関連疾患
Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition. -
配列類似性
Contains 1 EF-hand domain.
Contains 1 SAM (sterile alpha motif) domain. -
ドメイン
The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends. -
翻訳後修飾
Glycosylation is required for cell surface expression.
Phosphorylated predominantly on Ser residues. -
細胞内局在
Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules. - Information by UniProt
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参照データベース
- Entrez Gene: 20866 Mouse
- SwissProt: P70302 Mouse
- Unigene: 645 Mouse
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別名
- D11S4896E antibody
- GOK antibody
- OTTHUMP00000164512 antibody
see all
画像
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Western blot - Anti-Stromal interaction molecule 1 antibody (ab62031)Lane 1 : Anti-Stromal interaction molecule 1 antibody (ab62031) at 1 µg/ml
Lane 2 : Anti-Stromal interaction molecule 1 antibody (ab62031) at 2 µg/ml
All lanes : Mouse thymus tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 77 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 116 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody (ab62031)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse spleen tissue labeling Stromal interaction molecule 1 with Anti-Stromal interaction molecule 1 antibody (ab62031) at 5μg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-Stromal interaction molecule 1 antibody (ab62031)Immunofluorescence of Stromal interaction molecule 1 in mouse spleen cells with ab62031 at 20 μg/mL.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab62031 は 6 報の論文で使用されています。
- Luo Y et al. The miRNA-185-5p/STIM1 Axis Regulates the Invasiveness of Nasopharyngeal Carcinoma Cell Lines by Modulating EGFR Activation-Stimulated Switch from E- to N-Cadherin. Molecules 28:N/A (2023). PubMed: 36677874
- Wicher SA et al. Aging increases senescence, calcium signaling, and extracellular matrix deposition in human airway smooth muscle. PLoS One 16:e0254710 (2021). PubMed: 34324543
- Wei J et al. EB virus promotes metastatic potential by boosting STIM1-dependent Ca2+ signaling in nasopharyngeal carcinoma cells. Cancer Lett 478:122-132 (2020). PubMed: 32165272
- Sathish V et al. Cigarette Smoke and Estrogen Signaling in Human Airway Smooth Muscle. Cell Physiol Biochem 36:1101-15 (2015). PubMed: 26113109
- Vohra PK et al. TRPC3 regulates release of brain-derived neurotrophic factor from human airway smooth muscle. Biochim Biophys Acta 1833:2953-60 (2013). PubMed: 23899746
- Abcejo AJ et al. Brain-derived neurotrophic factor enhances calcium regulatory mechanisms in human airway smooth muscle. PLoS One 7:e44343 (2012). WB . PubMed: 22952960