Anti-Stromal interaction molecule 1 抗体 [5A2]

• ICC/IF

AbReview20217****

Immunocytochemistry/ Immunofluorescence - Anti-Stromal interaction molecule 1 antibody [5A2] (AB57834)

ab57834 staining the stromal interaction molecule in HeLa cells by immunocytochemistry/immunofluorescence (ICC/IF). Cells were fixed with paraformaldehyde and permeabilized with 0.5% TritonX. Samples were incubated with primary antibody (1/200) for 1 hour at 24°C. An Alexa Fluor ^® 488-conjugated chicken anti-mouse polyclonal (1/1000) was used as the secondary.

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This image is courtesy of an anonymous Abreview

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Stromal interaction molecule 1 antibody [5A2] (AB57834)

ab57834 at 10 ug/ml staining Stromal interaction molecule 1 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

This image was generated using the ascites version of the product.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [5A2] (AB57834)

Stromal interaction molecule 1 antibody (ab57834) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human malignant lymphoma, diffuse large B.

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• Flow Cyt

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Flow Cytometry - Anti-Stromal interaction molecule 1 antibody [5A2] (AB57834)

Overlay histogram showing HeLa cells stained with ab57834 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57834, 0.5μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

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• WB

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Western blot - Anti-Stromal interaction molecule 1 antibody [5A2] (AB57834)

Stromal interaction molecule 1 antibody (ab57834) at 1ug/lane + HeLa cell lysate at 25ug/lane.

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All lanes:

Western blot - Anti-Stromal interaction molecule 1 antibody [5A2] (ab57834)

Predicted band size: 77 kDa

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• WB

CiteAb

Western blot - Anti-Stromal interaction molecule 1 antibody [5A2] (AB57834)

Stromal interaction molecule 1 western blot using anti-Stromal interaction molecule 1 antibody [5A2] ab57834. Publication image and figure legend from Uysal-Onganer, P., MacLatchy, A., et al., 2020, Int J Mol Sci, PubMed 32098295.

ab57834 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab57834 please see the product overview.

PAD2 and PAD4 isozyme-specific inhibitor 1 h treatment affects prohibitin (PHB), stromal interaction molecule 1 (STIM-1) and moesin protein levels in LN18 and LN229 GBM cell lines. (A) PHB protein levels in LN18 and LN229 cells, respectively, following 1 h treatment with the PAD2 and PAD4 inhibitors, compared to control-treated cells. (B) STIM-1 protein levels in LN18 and LN229 cells respectively, following 1 h treatment with the PAD2 and PAD4 inhibitors, compared to control-treated cells. (C) Moesin protein levels in LN18 and LN229 cells, respectively, following 1 h treatment with the PAD2 and PAD4 inhibitors, compared to control-treated cells. Representative blots are shown; the density ratios of the various proteins analysed and actin are presented as normalised quantified data (mean ±S.D.) for treatment with the PAD2 and PAD4 specific inhibitors, compared to DMSO treated controls. The corresponding molecular weight size standard is indicated in kilodaltons (kDa) on each blot. Exact p-values are indicated, error bars show SD (* indicates significant differences with p < 0.05; n = 3 biological replicates for all).

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