Anti-Strep-tag II 抗体 [GT661] (ab184224)
![Western blot - Anti-Strep-tag II antibody [GT661] (ab184224)](https://www.abcam.co.jp/ps/products/184/ab184224/Images/ab184224-215425-anti-strep-tag-antibody-gt661-western-blot.jpg "Western blot - Anti-Strep-tag II antibody [GT661] (ab184224)")
Key features and details
- Mouse monoclonal [GT661] to Strep-tag II
- Suitable for: WB, IP, ICC/IF
- Reacts with: Species independent
- Isotype: IgG2a
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Strep-tag II antibody [GT661]
Strep-tag II 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [GT661] to Strep-tag II -
由来種
Mouse -
アプリケーション
適用あり: WB, IP, ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to Strep-tag II.
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ポジティブ･コントロール
- Strep-tagged protein expressing 293T cells.
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特記事項
This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
GT661 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184224の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000.
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| IP |
1/3000.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. |
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IP
1/3000. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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関連性
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II. -
別名
- anti-Strep-Tag II antibody
- Strep-Tag II antibody
- Strep-Tag II antibody antibody
- Strep-Tag IIantiboy antibody
画像
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Western blot - Anti-Strep-tag II antibody [GT661] (ab184224)All lanes : Anti-Strep-tag II antibody [GT661] (ab184224) at 1/5000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : whole cell lysate of strep-tagged II protein expressing 293T cells
Lysates/proteins at 30 µg per lane.12 % SDS-PAGE
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![Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [GT661] (ab184224)](https://www.abcam.co.jp/ps/products/184/ab184224/Images/ab184224-215421-anti-strep-tag-antibody-gt661-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [GT661] (ab184224)Immunofluorescent analysis of Strep II/GFP-tag transfected HeLa cells (fixed in 4% paraformaldehyde at RT for 15 min) showing labeling of Strep-recombinant protein by ab184224 used at 1/500 dilution. Red: Strep-recombinant protein stained by Strep-tag antibody (ab184224) diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.
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![Immunoprecipitation - Anti-Strep-tag II antibody [GT661] (ab184224)](https://www.abcam.co.jp/ps/products/184/ab184224/Images/ab184224-215420-anti-strep-tag-antibody-gt661-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Strep-tag II antibody [GT661] (ab184224)ab184224 immunoprecipitates strep tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract.
Lane 1 : 20 μg whole cell lysate/extract of strep II tagged protein expressing 293T cells
Lane 2 : Control with 2.5 μg of pre-immune mouse IgG
Lane 3 : Immunoprecipitation of strep tagged protein by 2.5 μg of strep tag antibody (ab184224).
10% SDS-PAGE -The immunoprecipitated strep tagged protein was detected by strep tag antibody (ab184224) diluted at 1/3000. Anti-mouse IgG (HRP) antibody was used as a secondary reagent.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab184224 は 12 報の論文で使用されています。
- Tan X et al. Coronavirus subverts ER-phagy by hijacking FAM134B and ATL3 into p62 condensates to facilitate viral replication. Cell Rep 42:112286 (2023). PubMed: 36952345
- Yue M et al. Coronaviral ORF6 protein mediates inter-organelle contacts and modulates host cell lipid flux for virus production. EMBO J 42:e112542 (2023). PubMed: 37218505
- Gil S et al. Homologs of Ancestral CNNM Proteins Affect Magnesium Homeostasis and Circadian Rhythmicity in a Model Eukaryotic Cell. Int J Mol Sci 24:N/A (2023). PubMed: 36768595
- De Caluwé L et al. The CD147 Protein Complex Is Involved in Entry of Chikungunya Virus and Related Alphaviruses in Human Cells. Front Microbiol 12:615165 (2021). PubMed: 33717005
- Sansico F et al. COVID-19 Specific Immune Markers Revealed by Single Cell Phenotypic Profiling. Biomedicines 9:N/A (2021). PubMed: 34944610