Anti-Stra8 抗体 (ab49602)
Key features and details
- Rabbit polyclonal to Stra8
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-Stra8 antibody
Stra8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Stra8 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Hamster
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免疫原
Synthetic peptide corresponding to Mouse Stra8 aa 1-100.
(Peptide available asab49601) -
ポジティブ･コントロール
- IHC-P: Mouse testis tissue. WB: CHO Overexpressing Stra8 (Mouse) Whole Cell Lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab49602の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (3) |
Use a concentration of 0.1 µg/ml. Recommended use at a low concentration otherwise high background staining may be observed
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| WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa). Ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa.
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| 特記事項 |
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IHC-P
Use a concentration of 0.1 µg/ml. Recommended use at a low concentration otherwise high background staining may be observed |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa). Ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa. |
ターゲット情報
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機能
Required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase. -
組織特異性
Expressed specifically in testis. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 20899 Mouse
- Entrez Gene: 500079 Rat
- SwissProt: P70278 Mouse
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別名
- Stimulated by retinoic acid 8 antibody
- stimulated by retinoic acid 8 homolog antibody
- Stimulated by retinoic acid Gene 8 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stra8 antibody (ab49602)IHC image of Stra8 staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49602, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Stra8 antibody (ab49602)All lanes : Anti-Stra8 antibody (ab49602) at 1 µg/ml
Lane 1 : CHO Overexpressing Stra8 (Mouse) Whole Cell Lysate
Lane 2 : CHO Negative Control Whole Cell Lysate
Lysates/proteins at 1 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa. -
Western blot - Anti-Stra8 antibody (ab49602)This image is courtesy of an anonymous abreview.All lanes : Anti-Stra8 antibody (ab49602) at 1/500 dilution
Lane 1 : WT D3 mouse ES cells cultured with LIF.
Lane 2 : WT D3 mouse ES cells cultured without LIF for 24 hours.
Lane 3 : WT D3 mouse ES cells cultured without LIF and with 1uM Retinoic acid for 24 hours.
Lysates/proteins at 200000 cells per lane.
Secondary
All lanes : Non-Abcam antibody was used: goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (83)
ab49602 は 83 報の論文で使用されています。
- Zhang FL et al. Single cell epigenomic and transcriptomic analysis uncovers potential transcription factors regulating mitotic/meiotic switch. Cell Death Dis 14:134 (2023). PubMed: 36797258
- Gantchev J et al. Ectopically Expressed Meiosis-Specific Cancer Testis Antigen HORMAD1 Promotes Genomic Instability in Squamous Cell Carcinomas. Cells 12:N/A (2023). PubMed: 37371097
- Wu Y et al. Fat mass and obesity-associated factor (FTO)-mediated N6-methyladenosine regulates spermatogenesis in an age-dependent manner. J Biol Chem 299:104783 (2023). PubMed: 37146971
- Peng YJ et al. Sertoli cells are the source of stem cell factor for spermatogenesis. Development 150:N/A (2023). PubMed: 36861441
- Yue M et al. Protopine Alleviates Dextran Sodium Sulfate-Induced Ulcerative Colitis by Improving Intestinal Barrier Function and Regulating Intestinal Microbiota. Molecules 28:N/A (2023). PubMed: 37446938