JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB227705

Anti-STING 抗体 [SP339]

Anti-STING antibody [SP339]

  • BOND RX™ Validated
  • 20ul selling size
  • KO Validated
  • RabMAb
  • Recombinant
  • 詳細を見る

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal STING antibody. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples. Cited in 2 publications.

別名を表示する

ERIS, MITA, STING, TMEM173, STING1, Stimulator of interferon genes protein, hSTING, Endoplasmic reticulum interferon stimulator, Mediator of IRF3 activation, Transmembrane protein 173, hMITA

18 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling TMEM173 with ab227705 at 1/100 dilution (2.5 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227705 for 10 mins at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)

Formalin-fixed, paraffin-embedded human lung tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TMEM173 with ab227705 at 1/100 dilution (2.5 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227705 for 10 mins at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-STING antibody [SP339] (AB227705)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-STING antibody [SP339] (AB227705)

Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling TMEM173 with ab227705 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)

Formalin-fixed, paraffin-embedded human colon tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-STING antibody [SP339] (AB227705)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-STING antibody [SP339] (AB227705)

Flow cytometry analysis of THP-1 (human acute monocytic leukemia) labeling TMEM173 with purified ab227705 at 1/20 dilution (12.5 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)

Formalin-fixed, paraffin-embedded human thymus tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)

Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (AB227705)

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-STING antibody [SP339] (AB227705)
  • WB

Lab

Western blot - Anti-STING antibody [SP339] (AB227705)

ab227705 was shown to react with STING1 in wild-type THP-1 cells in Western blot with loss of signal observed in STING1 knockout cell line ab270493. Wild-type THP-1 and STING1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab227705 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-STING antibody [SP339] (ab227705) at 1/1000 dilution

Lane 1:

Wild-type THP-1 lysate at 40 µg

Lane 2:

STING1 knock-out THP-1 lysate at 40 µg

false

Western blot - Anti-STING antibody [SP339] (AB227705)
  • WB

Supplier Data

Western blot - Anti-STING antibody [SP339] (AB227705)

All lanes:

Western blot - Anti-STING antibody [SP339] (ab227705) at 1/400 dilution

All lanes:

THP-1 (human monocytic leukemia cell line) cell lysate

Predicted band size: 42 kDa

false

Western blot - Anti-STING antibody [SP339] (AB227705)
  • WB

Lab

Western blot - Anti-STING antibody [SP339] (AB227705)

Lanes 1 - 4 : Merged signal (red and green). Green - ab227705 observed at 37 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab227705 was shown to react with TMEM173 in wild-type THP-1 cells in Western blot with loss of signal observed in TMEM173 knockout cell line ab270493 (TMEM173 knockout cell lysate ab270516). Wild-type THP-1 and TMEM173 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab227705 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-STING antibody [SP339] (ab227705) at 1/400 dilution

Lane 1:

Wild-type THP-1 cell lysate at 20 µg

Lane 2:

TMEM173 knockout THP-1 cell lysate at 20 µg

Lane 2:

Western blot - Human TMEM173 knockout THP-1 cell line (<a href='/products/cell-lines/human-tmem173-knockout-thp-1-cell-line-ab270493'>ab270493</a>)

Lane 3:

Human Tonsil tissue lysate at 20 µg

Lane 4:

Human Thymus tissue lysate at 20 µg

Predicted band size: 42 kDa

Observed band size: 37 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-STING antibody [SP339] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

SP339

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20 - 1/400", "FlowCytIntra-species-notes": "<p>Primary incubation for 30 minutes at 4°C.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/400", "WB-species-notes": "<p>Primary incubation for 1 hour at room temperature.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.</p><p>Primary incubation for 10 minutes at room temperature.</p>" }, "Cow": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A/G
精製に関する特記事項
Purified from TCS by protein A/G.
バッファー組成
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The stimulator of interferon genes (STING) also known as TMEM173 or MPYS is a critical transmembrane protein with a molecular weight of approximately 42 kDa. It is primarily expressed in the endoplasmic reticulum of various cell types including immune cells where it plays a central role in sensing cytosolic DNA. STING binds to cyclic dinucleotides produced by the enzyme cGAS upon recognition of aberrant DNA in the cytosol. This binding initiates activation and translocation of STING to the Golgi apparatus facilitating further signaling events.
Biological function summary

STING serves as a pivotal regulator in the innate immune response to viral and bacterial infections. It operates by forming a signaling complex with kinases and other effector proteins which subsequently leads to the activation of transcription factors such as IRF3 and NF-kB. These transcription factors then induce the expression of type I interferons and other cytokines important for mounting an effective antiviral response. The STING pathway therefore enhances the immune system's ability to detect and respond to pathogens.

Pathways

The activity of STING is integral to the cGAS-STING pathway a significant cytosolic DNA-sensing pathway involved in innate immunity. Upon activation STING interacts with TBK1 a kinase that further phosphorylates IRF3 promoting its nuclear translocation and activation. Beyond this STING also intersects with pathways involving autophagy a cellular process necessary for clearing pathogens and damaged cellular components. Through these pathways STING critically contributes to upholding cellular homeostasis and immune defense.

The dysregulation of STING is linked to autoinflammatory diseases and certain cancers. Abnormal STING activation can lead to chronic inflammation a feature observed in diseases such as STING-associated vasculopathy with onset in infancy (SAVI). STING's role in cancer is also notable where its ability to activate immune cells can be harnessed in immunotherapy yet its chronic activation may promote tumorigenesis. In cancer STING often interacts with proteins like K-Ras influencing tumor growth and response to therapies.

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta) (PubMed : 18724357, PubMed : 18818105, PubMed : 19433799, PubMed : 19776740, PubMed : 23027953, PubMed : 23747010, PubMed : 23910378, PubMed : 27801882, PubMed : 29973723, PubMed : 30842659, PubMed : 35045565, PubMed : 35388221, PubMed : 36808561, PubMed : 37832545). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm (PubMed : 26300263). Acts by binding cyclic dinucleotides : recognizes and binds cyclic di-GMP (c-di-GMP), a second messenger produced by bacteria, cyclic UMP-AMP (2',3'-cUAMP), and cyclic GMP-AMP (cGAMP), a messenger produced by CGAS in response to DNA virus in the cytosol (PubMed : 21947006, PubMed : 23258412, PubMed : 23707065, PubMed : 23722158, PubMed : 23747010, PubMed : 23910378, PubMed : 26229117, PubMed : 30842659, PubMed : 35388221, PubMed : 37379839). Upon binding to c-di-GMP, cUAMP or cGAMP, STING1 oligomerizes, translocates from the endoplasmic reticulum and is phosphorylated by TBK1 on the pLxIS motif, leading to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent anti-viral state (PubMed : 22394562, PubMed : 25636800, PubMed : 29973723, PubMed : 30842653, PubMed : 35045565, PubMed : 35388221). Exhibits 2',3' phosphodiester linkage-specific ligand recognition : can bind both 2'-3' linked cGAMP (2'-3'-cGAMP) and 3'-3' linked cGAMP but is preferentially activated by 2'-3' linked cGAMP (PubMed : 23747010, PubMed : 23910378, PubMed : 26300263). The preference for 2'-3'-cGAMP, compared to other linkage isomers is probably due to the ligand itself, whichs adopts an organized free-ligand conformation that resembles the STING1-bound conformation and pays low energy costs in changing into the active conformation (PubMed : 26150511). In addition to promote the production of type I interferons, plays a direct role in autophagy (PubMed : 30568238, PubMed : 30842662). Following cGAMP-binding, STING1 buds from the endoplasmic reticulum into COPII vesicles, which then form the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) (PubMed : 30842662). The ERGIC serves as the membrane source for WIPI2 recruitment and LC3 lipidation, leading to formation of autophagosomes that target cytosolic DNA or DNA viruses for degradation by the lysosome (PubMed : 30842662). Promotes autophagy by acting as a proton channel that directs proton efflux from the Golgi to facilitate MAP1LC3B/LC3B lipidation (PubMed : 37535724). The autophagy- and interferon-inducing activities can be uncoupled and autophagy induction is independent of TBK1 phosphorylation (PubMed : 30568238, PubMed : 30842662). Autophagy is also triggered upon infection by bacteria : following c-di-GMP-binding, which is produced by live Gram-positive bacteria, promotes reticulophagy (By similarity). May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons (PubMed : 18724357). May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II) (By similarity).. (Microbial infection) Antiviral activity is antagonized by oncoproteins, such as papillomavirus (HPV) protein E7 and adenovirus early E1A protein (PubMed : 26405230). Such oncoproteins prevent the ability to sense cytosolic DNA (PubMed : 26405230).
See full target information STING1

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

F1000Research 13:1049 PubMed40535171

2025

A guide to selecting high-performing antibodies for STING1 (Uniprot ID: Q86WV6) for use in western blot, immunoprecipitation, and immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Vera Ruíz Moleón,Charles Alende,Maryam Fotouhi,Riham Ayoubi,Kathleen Southern,Carl Laflamme

International journal of immunopathology and pharmacology 39:3946320251324821 PubMed40070172

2025

Dissociation between the expression of cGAS/STING and a senescence-associated signature in colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sofian Al Shboul,Ola Abu Al Karsaneh,Moath Alrjoub,Mohammad Al-Qudah,Mohammed El-Sadoni,Ahmad Alhesa,Mohannad Ramadan,Marwa Barukba,Esraa Fares Al-Quran,Amr Masaadeh,Farah N Almasri,Uruk Shahin,Moureq R Alotaibi,Mohammad Al-Azab,Ashraf I Khasawneh,Tareq Saleh
View all publications

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com