Anti-STELLAR 抗体 (ab19878)
Key features and details
- Rabbit polyclonal to STELLAR
- Suitable for: IHC-Fr, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-STELLAR antibody
STELLAR 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to STELLAR -
由来種
Rabbit -
特異性
From November 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: IHC-Fr, ICC/IFmore details
適用なし: WB -
種交差性
交差種: Mouse
交差が予測される動物種: Rat -
免疫原
Synthetic peptide corresponding to Mouse STELLAR aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab23324) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19878の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr | (2) |
Use a concentration of 4 µg/ml.
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ICC/IF | (2) |
Use a concentration of 5 µg/ml.
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特記事項 |
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IHC-Fr
Use a concentration of 4 µg/ml. |
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
May play a role in maintaining cell pluripotentiality. -
組織特異性
Low expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors. -
発生段階
Expressed in fetal ovary. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 73708 Mouse
- Entrez Gene: 297576 Rat
- SwissProt: Q8QZY3 Mouse
- SwissProt: Q6IMK0 Rat
- Unigene: 27982 Mouse
- Unigene: 79056 Rat
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製品の状態
Stella is a gene specifically expressed in primordial germ cells, oocytes, preimplantation embryos and pluripotent cells. It encodes a protein with a SAP-like domain and a splicing factor motif-like structure, suggesting possible roles in chromosomal organization or RNA processing. Embryos without Stella are compromised in preimplantation development and rarely reach the blastocyst stage. -
別名
- Compaction associated protein 1 antibody
- Developmental pluripotency associated 3 antibody
- Developmental pluripotency associated protein 3 antibody
see all
画像
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ab19878 staining STELLAR in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab19878 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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The image shows ab19878 staining of a crysosection of mouse embryonic genital ridges (E14.5). The samples were fixed overnight in 4% paraformaldehyde, permeabilised with 0.1% Triton and stained overnight at 4 degrees. 4µg/ml of antibody was used. Staining was in the nucleus and cytoplasm of Oct4-positive cells. The blue fluorescence is DAPI staining of DNA.
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ICC/IF image of ab19878 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19878, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (37)
ab19878 は 37 報の論文で使用されています。
- Chen Y et al. The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting. Protein Cell 14:477-496 (2023). PubMed: 36921016
- Yu L et al. Derivation and Primordial Germ Cell Induction of Intermediate Pluripotent Stem Cells. Methods Mol Biol 2677:269-280 (2023). PubMed: 37464248
- Lee J et al. Coordination of canonical and noncanonical Hedgehog signalling pathways mediated by WDR11 during primordial germ cell development. Sci Rep 13:12309 (2023). PubMed: 37516749
- Wei X et al. PGC7 Regulates Genome-Wide DNA Methylation by Regulating ERK-Mediated Subcellular Localization of DNMT1. Int J Mol Sci 24:N/A (2023). PubMed: 36834503
- Pei L et al. Rcor2 Is Required for Somatic Differentiation and Represses Germline Cell Fate. Stem Cells Int 2022:5283615 (2022). PubMed: 35345626