Anti-Stathmin 1 (phospho S16) 抗体 (ab47328)

Thank you for your reply. I am sorry that the antibodies did not work for you as stated on the datasheet. I have processed your request to receive refunds for all 3 of these antibodies. Your credit note number is ********. If there is anything else I can help you with, please let me know.

Thank you for contacting us. We have received your three western blot complaint questionnaires for ab47328, ab47398, and ab47399. These questionnaires refer to attachments with additional data, but unfortunately, we did not receive any attachments. It would be very helpful if you could please provide images of the blots for these antibodies, or tell me the molecular weights of the observed bands. Also, how long were the samples treated and what concentration of phosphatase was used when probing with ab47399? Milk contains endogenous phosphatases which may be causing the lack of specific signal observed with ab47328 and ab47398. If BSA has been giving poor results, you could try adding phosphatase inhibitors to your milk. These antibodies were validated using a reduced and denatured western blot protocol. Were the samples treated with SDS and a reducing agent such as BME or DTT prior to loading? It is possible that without being fully reduced and denatured (including boiling the samples for 5 minutes at 95C), the epitopes recognized by these antibodies may not exposed, which would prevent the antibody from binding effectively. I hope this helps, if not, please let me know and I will be happy to assist you further.

Thank you for contacting us. The single amino acid difference is due to a nomenclature difference, in which the first methionine of a protein may or may not be included. Therefore, our antibodies detect the correct phosphorylation sites. I hope this helpful to the customer.